LOCUS       sequence-231555-        8307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene constitutive expression from a Pc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis.
  JOURNAL   Appl Environ Microbiol. 2013 Apr;79(8):2836-40. doi: 
            10.1128/AEM.03746-12. Epub 2013 Feb 15.
  PUBMED    23416993
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03746-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-04"; volume: "79"; issue: "8"; pages: "2836-40"
COMMENT     Sequence Label: pMMPc
FEATURES             Location/Qualifiers
     source          1..8307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(537..554)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      707..793
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      885..912
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        931..1022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1023..1091,1092..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1241..1260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2320..2714
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3056..3143
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4382..5353
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5864..6703
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6690..7541
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
ORIGIN
        1 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
       61 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
      121 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
      181 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
      241 cgttgccgat acaagaacaa cagcgcgttg agcgcctgcc ggtgggtggc cggcgccact
      301 tgcttctcgg tggcgagcat ggtcagaaaa ccctcgactt cagcttgccc catttcgcgc
      361 ggatgtcgaa acccaccatg gctgcgggcc gtccacaaca caaatgcctt ggcccagtag
      421 acataagcct tctcggtctg taggctgtaa tgcaggtagc gaacccgtga attcccgggg
      481 atccgtcgac ctgcagccaa gcttggctgt tttggcggat gagagaagat tttcagcctg
      541 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
      601 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
      661 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      781 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
      841 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
      901 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
      961 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1021 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1081 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1141 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1201 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1261 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1321 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1381 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1441 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1501 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1561 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1621 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1681 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1741 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1801 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1861 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1921 atttctgaaa gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc gtagaaagcc
     1981 atgctccagc cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat agcccggcaa
     2041 ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg
     2101 atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct
     2161 ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac
     2221 agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac
     2281 attgccagcc agtgcccgtc actcccgtac taactgtcac gaacccctgc aataactgtc
     2341 acgcccccct gcaataactg tcacgaaccc ctgcaataac tgtcacgccc ccaaacctgc
     2401 aaacccagca ggggcggggg ctggcggggt gttggaaaaa tccatccatg attatctaag
     2461 aataatccac taggcgcggt tatcagcgcc cttgtggggc gctgctgccc ttgcccaata
     2521 tgcccggcca gaggccggat agctggtcta ttcgctgcgc taggctacac accgccccac
     2581 cgctgcgcgg cagggggaaa ggcgggcaaa gcccgctaaa ccccacacca aaccccgcag
     2641 aaatacgctg gagcgctttt agccgcttta gcggcctttc cccctacccg aagggtgggg
     2701 gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat tcagcccggc tcatccttct
     2761 ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac gcatccattg
     2821 ccgccatgag ccgatcctcc ggccactcgc tgctgttcac cttggccaaa atcatggccc
     2881 ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc cttgcccgca
     2941 cccgctgaat ttcggcattg attcgcgctc gttgttcttc gagcttggcc agccgatccg
     3001 ccgccttgtt gctcccctta accatcttga caccccattg ttaatgtgct gtctcgtagg
     3061 ctatcatgga ggcacagcgg cggcaatccc gaccctactt tgtaggggag ggcgcactta
     3121 ccggtttctc ttcgagaaac tggcctaacg gccacccttc gggcggtgcg ctctccgagg
     3181 gccattgcat ggagccgaaa agcaaaagca acagcgaggc agcatggcga tttatcacct
     3241 tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc agggccaagg ccgactacat
     3301 ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg aatccgggca
     3361 catgccggag ttcgtcgagc ggcccgccga ctactgggat gctgccgacc tgtatgaacg
     3421 cgccaatggg cggctgttca aggaggtcga atttgccctg ccggtcgagc tgaccctcga
     3481 ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg accggtgccg agcgcctgcc
     3541 gtatacgctg gccatccatg ccggtggcgg cgagaacccg cactgccacc tgatgatctc
     3601 cgagcggatc aatgacggca tcgagcggcc cgccgctcag tggttcaagc ggtacaacgg
     3661 caagaccccg gagaagggcg gggcacagaa gaccgaagcg ctcaagccca aggcatggct
     3721 tgagcagacc cgcgaggcat gggccgacca tgccaaccgg gcattagagc gggctggcca
     3781 cgacgcccgc attgaccaca gaacacttga ggcgcagggc atcgagcgcc tgcccggtgt
     3841 tcacctgggg ccgaacgtgg tggagatgga aggccggggc atccgcaccg accgggcaga
     3901 cgtggccctg aacatcgaca ccgccaacgc ccagatcatc gacttacagg aataccggga
     3961 ggcaatagac catgaacgca atcgacagag tgaagaaatc cagaggcatc aacgagttag
     4021 cggagcagat cgaaccgctg gcccagagca tggcgacact ggccgacgaa gcccggcagg
     4081 tcatgagcca gacccagcag gccagcgagg cgcaggcggc ggagtggctg aaagcccagc
     4141 gccagacagg ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta gccgccgagg
     4201 tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg aagctatggc
     4261 taaccgtgat gctggcttcc atgatgccta cggtggtgct gctgatcgca tcgttgctct
     4321 tgctcgacct gacgccactg acaaccgagg acggctcgat ctggctgcgc ttggtggccc
     4381 gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca tgggctgtga
     4441 gcgcttcgat atcggcgtca gggacgccac caccggccag atgatgaacc gggaatggtc
     4501 agccgccgaa gtgctccaga acacgccatg gctcaagcgg atgaatgccc agggcaatga
     4561 cgtgtatatc aggcccgccg agcaggagcg gcatggtctg gtgctggtgg acgacctcag
     4621 cgagtttgac ctggatgaca tgaaagccga gggccgggag cctgccctgg tagtggaaac
     4681 cagcccgaag aactatcagg catgggtcaa ggtggccgac gccgcaggcg gtgaacttcg
     4741 ggggcagatt gcccggacgc tggccagcga gtacgacgcc gacccggcca gcgccgacag
     4801 ccgccactat ggccgcttgg cgggcttcac caaccgcaag gacaagcaca ccacccgcgc
     4861 cggttatcag ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca ccgctggccc
     4921 ggcgctggtg cagcaggctg gccagcagat cgagcaggcc cagcggcagc aggagaaggc
     4981 ccgcaggctg gccagcctcg aactgcccga gcggcagctt agccgccacc ggcgcacggc
     5041 gctggacgag taccgcagcg agatggccgg gctggtcaag cgcttcggtg atgacctcag
     5101 caagtgcgac tttatcgccg cgcagaagct ggccagccgg ggccgcagtg ccgaggaaat
     5161 cggcaaggcc atggccgagg ccagcccagc gctggcagag cgcaagcccg gccacgaagc
     5221 ggattacatc gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc agcttgcgcg
     5281 ggccgagctg gcacgggcac cggcaccccg ccagcgaggc atggacaggg gcgggccaga
     5341 tttcagcatg tagtgcttgc gttggtactc acgcctgtta tactatgagt actcacgcac
     5401 agaagggggt tttatggaat acgaaaaaag cgcttcaggg tcggtctacc tgatcaaaag
     5461 tgacaagggc tattggttgc ccggtggctt tggttatacg tcaaacaagg ccgaggctgg
     5521 ccgcttttca gtcgctgata tggccagcct taaccttgac ggctgcacct tgtccttgtt
     5581 ccgcgaagac aagcctttcg gccccggcaa gtttctcggt gactgatatg aaagaccaaa
     5641 aggacaagca gaccggcgac ctgctggcca gccctgacgc tgtacgccaa gcgcgatatg
     5701 ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc gacgacgaat
     5761 acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg ggtagtgacc
     5821 ccgccagcgc ctaaccacca actgcctgca aaggaggcaa tcaatggcta cccataagcc
     5881 tatcaatatt ctggaggcgt tcgcagcagc gccgccaccg ctggactacg ttttgcccaa
     5941 catggtggcc ggtacggtcg gggcgctggt gtcgcccggt ggtgccggta aatccatgct
     6001 ggccctgcaa ctggccgcac agattgcagg cgggccggat ctgctggagg tgggcgaact
     6061 gcccaccggc ccggtgatct acctgcccgc cgaagacccg cccaccgcca ttcatcaccg
     6121 cctgcacgcc cttggggcgc acctcagcgc cgaggaacgg caagccgtgg ctgacggcct
     6181 gctgatccag ccgctgatcg gcagcctgcc caacatcatg gccccggagt ggttcgacgg
     6241 cctcaagcgc gccgccgagg gccgccgcct gatggtgctg gacacgctgc gccggttcca
     6301 catcgaggaa gaaaacgcca gcggccccat ggcccaggtc atcggtcgca tggaggccat
     6361 cgccgccgat accgggtgct ctatcgtgtt cctgcaccat gccagcaagg gcgcggccat
     6421 gatgggcgca ggcgaccagc agcaggccag ccggggcagc tcggtactgg tcgataacat
     6481 ccgctggcag tcctacctgt cgagcatgac cagcgccgag gccgaggaat ggggtgtgga
     6541 cgacgaccag cgccggttct tcgtccgctt cggtgtgagc aaggccaact atggcgcacc
     6601 gttcgctgat cggtggttca ggcggcatga cggcggggtg ctcaagcccg ccgtgctgga
     6661 gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc taagaacaag cacagcctca
     6721 gccacgtccg gcacgacccg gcgcactgtc tggcccccgg cctgttccgt gccctcaagc
     6781 ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac ggcaagcgga
     6841 tcgagttcag cggcccggag ccgctgggcg ctgatgatct gcgcatcctg caagggctgg
     6901 tggccatggc tgggcctaat ggcctagtgc ttggcccgga acccaagacc gaaggcggac
     6961 ggcagctccg gctgttcctg gaacccaagt gggaggccgt caccgctgat gccatggtgg
     7021 tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat agtggtgggg
     7081 cgctcaagca catacaggac tgcatcgagc gcctttggaa ggtatccatc atcgcccaga
     7141 atggccgcaa gcggcagggg tttcggctgc tgtcggagta cgccagcgac gaggcggacg
     7201 ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt ggcggccagc
     7261 atgtgcgcat cagcatggac gaggtgcggg cgctggacag cgaaaccgcc cgcctgctgc
     7321 accagcggct gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc atagatacct
     7381 tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag cgccgccagc
     7441 gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg gacggtaacc gagttcgcgg
     7501 cgggcaagta cgacatcacc cggcccaagg cggcaggctg acccccccca ctctattgta
     7561 aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt ggtaatacca
     7621 tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc tactgagcgc
     7681 tgccgcacag ctccataggc cgctttcctg gctttgcttc cagatgtatg ctcttctgct
     7741 cccgaacgcc agcaagacgt agcccagcgc gtcggccagc ttgcaattcg cgctaactta
     7801 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
     7921 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     7981 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     8041 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     8101 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
     8161 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
     8221 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
     8281 cgagtgagat atttatgcca gccagcc
//
