LOCUS       sequence-231556-       13854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human KIT L576P mutant and zebrafish mitfa specifically in
            zebrafish melanocytes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 13854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR mitfa:KIT L576P
FEATURES             Location/Qualifiers
     source          1..13854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3017..3033
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3043..3062
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3043..3061
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4830..4838
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(6076..6092)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6129..6147)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(6176..6200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6202..6336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6399..6419)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6427..6443)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6459..6593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(11384..11571,11572..11583)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11617..11633)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11670..11688)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11709..11725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11722..11744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11733..11749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11757..11763,11764..11781,11782..11787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11802..11823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11940..11957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12111..12699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12191..12210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12870..13661,13662..13730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13493..13512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13731..13835)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct
     1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt
     1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt
     1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt
     1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca
     1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg
     1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt
     1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa
     1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat
     1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt
     1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat
     1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta
     1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat
     1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat
     1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac
     1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa
     2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat
     2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat
     2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac
     2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga
     2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt
     2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac
     2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt
     2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat
     2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca
     2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt
     2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac
     2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc
     2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt
     2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg
     2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc
     2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt
     3001 acaaaaaagc aggcttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     3061 ggcgaattgg gtaccgggcc ccccctcgag gtcgacgcca ccatgagagg cgctcgcggc
     3121 gcctgggatt ttctctgcgt tctgctccta ctgcttcgcg tccagacagg ctcttctcaa
     3181 ccatctgtga gtccagggga accgtctcca ccatccatcc atccaggaaa atcagactta
     3241 atagtccgcg tgggcgacga gattaggctg ttatgcactg atccgggctt tgtcaaatgg
     3301 acttttgaga tcctggatga aacgaatgag aataagcaga atgaatggat cacggaaaag
     3361 gcagaagcca ccaacaccgg caaatacacg tgcaccaaca aacacggctt aagcaattcc
     3421 atttatgtgt ttgttagaga tcctgccaag cttttccttg ttgaccgctc cttgtatggg
     3481 aaagaagaca acgacacgct ggtccgctgt cctctcacag acccagaagt gaccaattat
     3541 tccctcaagg ggtgccaggg gaagcctctt cccaaggact tgaggtttat tcctgacccc
     3601 aaggcgggca tcatgatcaa aagtgtgaaa cgcgcctacc atcggctctg tctgcattgt
     3661 tctgtggacc aggagggcaa gtcagtgctg tcggaaaaat tcatcctgaa agtgaggcca
     3721 gccttcaaag ctgtgcctgt tgtgtctgtg tccaaagcaa gctatcttct tagggaaggg
     3781 gaagaattca cagtgacgtg cacaataaaa gatgtgtcta gttctgtgta ctcaacgtgg
     3841 aaaagagaaa acagtcagca gactaaacta caggagaaat ataatagctg gcatcacggt
     3901 gacttcaatt atgaacgtca ggcaacgttg actatcagtt cagcgagagt taatgattct
     3961 ggagtgttca tgtgttatgc caataatact tttggatcag caaatgtcac aacaaccttg
     4021 gaagtagtag ataaaggatt cattaatatc ttccccatga taaacactac agtatttgta
     4081 aacgatggag aaaatgtaga tttgattgtt gaatatgaag cattccccaa acctgaacac
     4141 cagcagtgga tctatatgaa cagaaccttc actgataaat gggaagatta tcccaagtct
     4201 gagaatgaaa gtaatatcag atacgtaagt gaacttcatc taacgagatt aaaaggcacc
     4261 gaaggaggca cttacacatt cctagtgtcc aattctgacg tcaatgctgc catagcattt
     4321 aatgtttatg tgaatacaaa accagaaatc ctgacttacg acaggctcgt gaatggcatg
     4381 ctccaatgtg tggcagcagg attcccagag cccacaatag attggtattt ttgtccagga
     4441 actgagcaga gatgctctgc ttctgtactg ccagtggatg tgcagacact aaactcatct
     4501 gggccaccgt ttggaaagct agtggttcag agttctatag attctagtgc attcaagcac
     4561 aatggcacgg ttgaatgtaa ggcttacaac gatgtgggca agacttctgc ctattttaac
     4621 tttgcattta aaggtaacaa caaagagcaa atccatcccc acaccctgtt cactcctttg
     4681 ctgattggtt tcgtaatcgt agctggcatg atgtgcatta ttgtgatgat tctgacctac
     4741 aaatatttac agaaacccat gtatgaagta cagtggaagg ttgttgagga gataaatgga
     4801 aacaattatg tttacataga cccaacacaa cctccttatg atcacaaatg ggagtttccc
     4861 agaaacaggc tgagttttgg gaaaaccctg ggtgctggag ctttcgggaa ggttgttgag
     4921 gcaactgctt atggcttaat taagtcagat gcggccatga ctgtcgctgt aaagatgctc
     4981 aagccgagtg cccatttgac agaacgggaa gccctcatgt ctgaactcaa agtcctgagt
     5041 taccttggta atcacatgaa tattgtgaat ctacttggag cctgcaccat tggagggccc
     5101 accctggtca ttacagaata ttgttgctat ggtgatcttt tgaatttttt gagaagaaaa
     5161 cgtgattcat ttatttgttc aaagcaggaa gatcatgcag aagctgcact ttataagaat
     5221 cttctgcatt caaaggagtc ttcctgcagc gatagtacta atgagtacat ggacatgaaa
     5281 cctggagttt cttatgttgt cccaaccaag gccgacaaaa ggagatctgt gagaataggc
     5341 tcatacatag aaagagatgt gactcccgcc atcatggagg atgacgagtt ggccctagac
     5401 ttagaagact tgctgagctt ttcttaccag gtggcaaagg gcatggcttt cctcgcctcc
     5461 aagaattgta ttcacagaga cttggcagcc agaaatatcc tccttactca tggtcggatc
     5521 acaaagattt gtgattttgg tctagccaga gacatcaaga atgattctaa ttatgtggtt
     5581 aaaggaaacg ctcgactacc tgtgaagtgg atggcacctg aaagcatttt caactgtgta
     5641 tacacgtttg aaagtgacgt ctggtcctat gggatttttc tttgggagct gttctcttta
     5701 ggaagcagcc cctatcctgg aatgccggtc gattctaagt tctacaagat gatcaaggaa
     5761 ggcttccgga tgctcagccc tgaacacgca cctgctgaaa tgtatgacat aatgaagact
     5821 tgctgggatg cagatcccct aaaaagacca acattcaagc aaattgttca gctaattgag
     5881 aagcagattt cagagagcac caatcatatt tactccaact tagcaaactg cagccccaac
     5941 cgacagaagc ccgtggtaga ccattctgtg cggatcaatt ctgtcggcag caccgcttcc
     6001 tcctcccagc ctctgcttgt gcacgacgat gtcttgtaat cgataagctt gatatcgaat
     6061 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc
     6121 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagcaccca
     6181 gctttcttgt acaaagtggg ggatccagac atgataagat acattgatga gtttggacaa
     6241 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     6301 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     6361 atgtttcagg ttcaggggga ggtgtgggag gttttttcca actttattat acatagttga
     6421 taattcactg gccgtcgttt tacggtacca tcgatgatga tccagacatg ataagataca
     6481 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6541 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     6601 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     6661 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag atctcagatt
     6721 gtatccaaaa tatcattttt tgtgttttaa agatgaacaa aggtctcagg gaattggaac
     6781 gacatgagga tgattaatta ataaaaaaaa attcattttt gggtgaacta tccctaaaat
     6841 caaagcaatt tgggcatttt ttttctaatt attatggaga acttgcatca cagctcttcc
     6901 actagaaaat aaaacaaatt tcagaaatac tgcagaaata cttttggact taatgttaaa
     6961 taacaatgta aaaagttttt ccagggcatc gtggtggtgc agtgggtagc attgtcgcct
     7021 tacagcaaga aggtcgctgg ttcgagactt ggttgggtca tttctgtgtg gagtttgcat
     7081 gttctcccca tgttcgtgtg ggtttcctcc gggtgctcca gtttcaccta cagcccaaag
     7141 acatgcgcta taggtgaatt gggtaagcta aattgtctaa agtgaatggt actgtggtgg
     7201 gtgtttccca gtgatgggtt gcagctgcaa gggcatccgc tggatttgtt tgttggcata
     7261 agttggcagt tcattcaact gtggcaaccc ctgattaata aaggggctaa gccaaaaaga
     7321 aaatgaatga ctgaatgaaa gtgaatccag ttgtacatgc ctagacatgt cctccaccct
     7381 tgcaagatgt acaccccaag aaatggagct gcatttgagg acaacaataa gccaagcctg
     7441 caaataaaag acacctcaac agtttttatt caagctactc tttatatcat ccgcgagaat
     7501 ccttcaaaaa tcaactaatt gtttacacga tttcattctt gactagactc tgacatgata
     7561 aatgaaaaaa tatatacaca agcacactaa atattctgaa atacaagatt aatactttac
     7621 gaaattgcgc tcttcatgat ctggatgtaa cactggtact tacaagacac acacacacac
     7681 aaatctaatt gtctaacttt ggttcatgaa atttagttgg cattgctaac agccattgtc
     7741 atgttcgtcc atactgctgc tgccgctact gttggaaggt actggggatc cggaggacaa
     7801 cagcgggtcg ctgctgccca ccggagacag ggtgttgtcc ataagcatgt cctccagctt
     7861 ggtggaggcc ttgtttgggc tgtcgtagac ccccggctcg ccgtcctccg tgggactgtc
     7921 attgtagctg atggtgccgt tgttgaggtc cagagtggta ggacgggaca tgtcaggact
     7981 gggaaggtgt gggtacaaat tggatgtgca gtcccccatc ccaggctctt gtttgattgc
     8041 tcgagccact aactcagcgg aataaaggct ggaagaagct acaacggtga gtccatgagc
     8101 acgggcttgc atttcaagtt cctgaatacg gagcaggaga tgtctgtttg cgtgttctag
     8161 tctcttctgt ctgttttcca gctcttttgc tttctgctgc tctttctgca atttcctaat
     8221 gtaatccact gatgctttca ggatggtgcc tttattccac ctcatgtctg gatcatttga
     8281 cttgggaatt aaagtcccca gctccttaat tctgtcattg atattaaacc gccttcttcg
     8341 ttcaatgagg ttgtggttgt ccttcttttg tctctctttt gccagggctc tgacttctgc
     8401 ttctactgga aagccatcag gtctttgata agagtcaaac ttgccacatg gtccagcttt
     8461 gtccattatg tgcatcatgc ccggagatgg agtaacggat aattcccttt tgacggccgg
     8521 caggcttgag gggcaggagt tactgatgga aactccagct ggaggaagag catgattgct
     8581 gtacatgtcc agcaggtttg ctgaaactgg aatcgtgttt gtcatttgaa gtcctgcatc
     8641 catgaaccca agaatgtcat cactgtagct tgattccaaa cttataatgt cttcaattac
     8701 atcgtccatc tctttctcac agttgagggt gagaagggcc atagggctgt tgggagcgct
     8761 ggctccgggt cccggggtca tgccgtgctc ggcgggctgg ctggggccgg gccctgtgct
     8821 ggcctgtggg ctcagcttgg ctcccagtgc actggacagg tagtgcttca cctgctgcct
     8881 ctggctctgc tggatgtggt acttcgaggg ggtttccagg tgggtctgaa cctggtagtg
     8941 actgtactcg agcatctcca acatgttcaa ctatgtgtta gcttcaaaaa agtattccct
     9001 ttgtgaattt aggtgatgtg caagaactga ccagtcgtct tggccagctg aagatgtcaa
     9061 tgccaaagaa aagacacaaa atgtatttaa gggggtggga ggggattgct cttctcatat
     9121 tgtttttttt acccaaacga tgaatttatg cctttataaa gttcatttga cgtcaccggt
     9181 ccgattcgac aaaagcctct ttgagacgat gggttactag tgtctaaact gaaactggct
     9241 tacggctaac taacgtttca tctttagttc cactcatcct gcataaaaca ttgtttgttc
     9301 tcagcatccg gcatacaatc actagcactg ttggtctaaa aacaagacag tttaagatgg
     9361 agttatctct gaaatgttga agagcaaaag ataggaaaga gaactttgtt gtctgatggg
     9421 cctttggtaa ttgcattttt ttcttaagag ttgaaatatt gtgcacacgt atgtaagtgc
     9481 gcacaaacaa aatcacagac gtacagttca tattcagatt catattcatt tttttttcta
     9541 caatgatgcc tcatgtacat catcttatta tcttacgtgt catttacagt caaaaaaaaa
     9601 ctagctggtt gaataaaagt aactttaagt cagaatttgc cagacgtatg aacactttgg
     9661 ggcttgattg taagtctttt tttttttttt gttgttgttg gatgtactgg cacaattatc
     9721 atcagagtaa atcagtaact gttgtgcatt tgcacagaaa gcatagaaat gaactgtagt
     9781 taaaaaagct aacaatcata aacaaagtag catgtgcata tactgtactg aatttttttt
     9841 aaataataat tgacataatc actggttgta atgtgtaaaa aatgagtctg ccttaaccca
     9901 acttccactc taaattgtgt gttgatgctc atttcccaaa agggctgaat tacgatagaa
     9961 aagataagtc aacagtttgt tgtctctgca ttgtttgagt tgactacatc ttgagcaaat
    10021 gtagctattg taaaatgggg atgagtatgt ggtacagaga accatctcgt caatggagtg
    10081 gaaactgcat taacatagag agatagagag aacagctgtg tctaggcatg gggtataaga
    10141 ttctgatggt atgataacct ttgataaaaa tatcacagtt tgatggtatt gtgattgcca
    10201 ctctaaaata tattattttt tttaatgtct tagtaaaaaa caaaaacttt aaacataata
    10261 tattttattt tgagaaacat tgaaaaaata ttttggaaca gaaaacatgt caagcttaat
    10321 aatttcaatg aatgatcgac ttctgctgtc ttcattagtt tcaaaaacag atttctttac
    10381 aatttaaaaa gcatttttgt atctcttttc tgctgaaaat gctgttgtcc taaaaaacaa
    10441 aaaaataaaa aataaataaa aaatcttaaa tataccttag gaacggtata gcagaaaata
    10501 ttggtggttt taaacccttg actttttcaa accggggtat accttgaaaa cggttatcgt
    10561 cctatgccta gctttgtcaa caaccaaaaa gcaactttat taacatctta aggcaagttt
    10621 aaaggcaaca tctaaaacag aatgaacggt tggagtttag tgaagtttat ttataaacta
    10681 atttcgagag aatcacgtgc ttatgactga ctacagctgg tcccacatta gcctatgtat
    10741 gattcaccaa tcagacaatt cctaactcat cataggccgc cagagttcct ttcatttcct
    10801 cgaaagagcg gcacagcagc ccagttgtta gcactgttgc ctcaaagcaa gaacgtcact
    10861 ggtccaagtc tctaacaggc cagtccatgg ttctgtgtgg agtttacatg ttgtcctcgt
    10921 gctcatgtgg atatcccctg ggttctccgg ttttctccta cagtccaaaa acttgcgaca
    10981 taggtgaatt gaacaaaacc aaattagcat agacaagctc ctaatcagca gaatatctct
    11041 ccgtagcaat ctcttattcg agatctgcga agatacggcc acgggtgctc ttgatcctgt
    11101 ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc ctccattttc
    11161 ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg tgcattgtgg
    11221 taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga ccaatttcat
    11281 ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa ctgggcatca
    11341 gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat ctccaaaaaa
    11401 taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt ttttctaaaa
    11461 aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt aaagtaaaaa
    11521 tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta ctttacacct
    11581 ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct agagcggccg
    11641 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
    11701 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    11761 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    11821 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    11881 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    11941 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    12001 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    12061 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    12121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    12181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    12241 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    12301 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    12361 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    12421 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    12481 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    12541 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    12601 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    12661 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    12721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag gcattttggt catgagatta
    12781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    12841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    12901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    12961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    13021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    13081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    13141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    13201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    13261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    13321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    13381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    13441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    13501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    13561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    13621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    13681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    13741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    13801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtg
//
