LOCUS       sequence-231557-       10205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector 
            (for BRET and LuC normalization).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti PA-NL
FEATURES             Location/Qualifiers
     source          1..10205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..103
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    401..518
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        583..1794
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1631..1650
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1832..2005
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2006..2179
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             2207..2236
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2237..2749
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     misc_feature    2858..3446
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2911..2931)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3329..3340)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3449..3465)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3450..3466)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3971..4151
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4177..4194)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4183..4407
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4453..4881
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4540..4559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4750..4771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4890..4910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4895..5224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5075..5210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5137..5156
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5272..5319
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5338..5712
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5842..5963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5879..5898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5933..5952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6012..6028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6012..6028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6025..6047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6036..6052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6060..6066,6067..6084,6085..6090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6105..6126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6243..6260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6414..7002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6494..6513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7173..7964,7965..8033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7796..7815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8034..8138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8213..8232)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8404..8783
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8785..8983
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8938..8958
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9001..9181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9228..9353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9846..10079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
       61 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      121 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      181 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      241 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      301 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
      361 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      421 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      481 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      541 gagatccagt ttggttaatt aacccgtgtc ggctccagat ctggcctccg cgccgggttt
      601 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
      661 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
      721 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
      781 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
      841 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
      901 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
      961 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     1021 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     1081 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     1141 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     1201 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     1261 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     1321 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     1381 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     1441 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     1501 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     1561 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     1621 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     1681 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     1741 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct
     1801 tgggcccggg aattaattcg ctagcaccat ggtggacaac aaattcaaca aagaacaaca
     1861 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     1921 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     1981 gctaaatgat gctcaggcgc cgaaagtaga caacaaattc aacaaagaac aacaaaacgc
     2041 gttctatgag atcttacatt tacctaactt aaacgaagaa caacgaaacg ccttcatcca
     2101 aagtttaaaa gatgacccaa gccaaagcgc taacctttta gcagaagcta aaaagctaaa
     2161 tggtgctcag gcgccgaaag tagacgcgaa ctcggaattc accatggaac agaaactgat
     2221 ctctgaagaa gacctggtct tcacactcga agatttcgtt ggggactggc gacagacagc
     2281 cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct
     2341 cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat
     2401 cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga
     2461 aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta
     2521 tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta
     2581 tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg
     2641 caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac
     2701 catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtgaa agcttggtac
     2761 cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg
     2821 ccgctcgagt ctagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2881 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2941 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3001 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3061 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3121 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3181 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3241 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3301 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3361 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3421 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     3481 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     3541 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     3601 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     3661 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     3721 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     3781 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     3841 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     3901 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     3961 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4021 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4081 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4141 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4201 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4261 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4321 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4381 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4441 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4861 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5161 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5221 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     5281 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     5341 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     5401 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     5461 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     5521 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     5581 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     5641 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     5701 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5761 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5821 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5941 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6001 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6061 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8161 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     8221 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8281 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8341 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     8401 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     8461 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     8521 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     8581 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     8641 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     8701 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     8761 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     8821 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     8881 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     8941 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     9001 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9061 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     9241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     9301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     9481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     9541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     9601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     9721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    10201 gaaat
//
