LOCUS       sequence-231642-        9326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. pyogenes Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Collias D, Marshall R, Collins SP, Beisel CL, Noireaux V
  TITLE     An educational module to explore CRISPR technologies with a 
            cell-free transcription-translation system.
  JOURNAL   Synth Biol (Oxf). 2019 Jan 21;4(1):ysz005. doi: 
            10.1093/synbio/ysz005. eCollection 2019.
  PUBMED    32995532
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2019-01-21"; volume: "4(1):ysz005. doi"; pages: " 
            10.1093/synbio/ysz005. eCollection 2019"
COMMENT     Sequence Label: p15A_SpyCas9_CmR
FEATURES             Location/Qualifiers
     source          1..9326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(67..72,73..89,90..95)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(413..491)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             787..4893
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             787..4890
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4914..5045
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   5046..5081
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   5112..5147
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(5576..5595)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(7448..8107)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8022..8041
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8108..8210)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8602..8619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8736..9281)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8816..8835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
       61 tcatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac agttaaattg
      121 ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac
      181 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
      241 ggattacgaa atcatcctgt ggagcttagt aggtttagca agatggcagc gcctaaatgt
      301 agaatgataa aaggattaag agattaattt ccctaaaaat gataaaacaa gcgttttgaa
      361 agcgcttgtt tttttggttt gcagtcagag tagaatagaa gtatcaaaaa aagcaccgac
      421 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatgctgttt
      481 tgaatggttc caacaagatt attttataac ttttataaca aataatcaag gagaaattca
      541 aagaaattta tcagccataa aacaatactt aatactatag aatgataaca aaataaacta
      601 ctttttaaaa gaattttgtg ttataatcta tttattatta agtattgggt aatatttttt
      661 gaagagatat tttgaaaaag aaaaattaaa gcatattaaa ctaatttcgg aggtcattaa
      721 aactattatt gaaatcatca aactcattat ggatttaatt taaacttttt attttaggag
      781 gcaaaaatgg ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg
      841 gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca
      901 gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca
      961 gcggaagcga ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt
     1021 atttgttatc tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt
     1081 catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt
     1141 tttggaaata tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg
     1201 cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta
     1261 gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat
     1321 agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa
     1381 aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa
     1441 tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt
     1501 gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg
     1561 gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta
     1621 ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat
     1681 gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca
     1741 gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta
     1801 gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga
     1861 tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca
     1921 attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg
     1981 ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag
     2041 ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag
     2101 aagattgaaa aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc
     2161 aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt
     2221 gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt
     2281 gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt
     2341 acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca
     2401 tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa
     2461 gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt
     2521 gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta
     2581 aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat
     2641 attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca
     2701 tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt
     2761 tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca
     2821 atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat
     2881 gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat
     2941 agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta
     3001 cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat
     3061 atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga
     3121 gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag
     3181 catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat
     3241 ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc
     3301 gatcacattg ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg
     3361 cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag
     3421 atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat
     3481 aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa
     3541 cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc
     3601 atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta
     3661 aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt
     3721 aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt
     3781 aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt
     3841 cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt
     3901 tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc
     3961 aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga
     4021 gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca
     4081 gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag
     4141 cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg
     4201 gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa
     4261 tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg
     4321 attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta
     4381 cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga
     4441 gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta
     4501 gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt
     4561 gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga attttctaag
     4621 cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga
     4681 gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt
     4741 ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct
     4801 acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca
     4861 cgcattgatt tgagtcagct aggaggtgac tgaagtatat tttagatgaa gattatttct
     4921 taataactaa aaatatggta taatactctt aataaatgca gtaatacagg ggcttttcaa
     4981 gactgaagtc tagctgagac aaatagtgcg attacgaaat tttttagaca aaaatagtct
     5041 acgaggtttt agagctatgc tgttttgaat ggtcccaaaa ctgagaccag tctcggaagc
     5101 tcaaaggtct cgttttagag ctatgctgtt ttgaatggtc ccaaaacttc agcacactga
     5161 gacttgttga gttccatgtt ttagagctat gctgttttga atggactcca ttcaacattg
     5221 ccgatgataa cttgagaaag agggttaata ccagcagtcg gataccttcc tattctttct
     5281 gttaaagcgt tttcatgtta taataggcaa aagaagagta gtgtgatcgt ccattccgac
     5341 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
     5401 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
     5461 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc
     5521 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     5581 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     5641 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     5701 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     5761 ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca
     5821 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     5881 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     5941 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     6001 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     6061 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     6121 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     6181 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     6241 ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac ggcgatttat
     6301 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     6361 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
     6421 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
     6481 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
     6541 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
     6601 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
     6661 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
     6721 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
     6781 cccctacgtg ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga
     6841 tactatgact gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt
     6901 ccgcaccgct gccggtagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     6961 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc ccaacagtcc
     7021 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgccctgca ccattatgtt
     7081 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
     7141 gaagcgctaa ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt
     7201 attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc
     7261 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     7321 cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc
     7381 gcttattatc acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt
     7441 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     7501 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     7561 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     7621 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     7681 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     7741 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     7801 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     7861 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     7921 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     7981 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     8041 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     8101 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     8161 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     8221 ttcgccaaaa gttggcccgg ggcttcccgg tatcaacagg gacaccagga tttatttatt
     8281 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     8341 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     8401 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     8461 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     8521 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     8581 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     8641 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     8701 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     8761 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     8821 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     8881 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     8941 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     9001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     9061 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     9121 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     9181 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     9241 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     9301 agataaaata tttctagatt tcagtg
//
