LOCUS       sequence-231648-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1084 GFP-TDP43 deltaCTD
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        187..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        491..694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     645..665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     691..715
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      805..814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2354..2378)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2414..3002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2467..2487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2885..2896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3016..3515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3051..3074)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3448..3467
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3536..4135
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3536..3555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4032..4052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4273..4506
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4578..4699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4615..4634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4669..4688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4739..4874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4894..4913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4895..4913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4923..4940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4923..4939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4932..4954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5081..5536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5168..5187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5378..5399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5562..5666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5667..5735,5736..6527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5885..5904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7187..7206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7440..7457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7574..7595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7610..7615,7616..7633,7634..7640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7648..7664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7653..7675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7672..7688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7672..7688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7707..7727
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7709..7727
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7755..7981
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7982..8162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8209..8334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8827..9060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9245..9289
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
       61 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      121 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat aagcttggga
      181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      601 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      661 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      721 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctagt ccagtgtggt
      781 ggaattctgc agattaccac cggtgccacc atgggaagta aaggagagga actatttaca
      841 ggtactgtgc ctatcaaagt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      901 cgtggcgagg gcgagggcga tgccaccgag ggcaagctga ccctgaagtt catctgcacc
      961 accggcaagc tgccggtgcc gtggccgacc ctcgtgacca ccctgaccta cggcgtgcag
     1021 tgcttcagcc gctacccgga ccacatgaag cgtcacgact tcttcaagtc cgccatgccg
     1081 gaaggctacg tccaggagcg caccatcgag ttcaaggacg acggcaccta caagacccgc
     1141 gcggaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcaacgac
     1201 ttcaaggagg acggcaacat cctgggtcac aagctggagt acaaccacaa cagccacaac
     1261 gtccgtatcg aagccgacaa gcagaagaac ggcatcaagg ctaacttcaa gatccgccac
     1321 aacgttgagg acggcagcca gcaggaagcc gaccacaaac agcagaacac cccgatcggc
     1381 gacggcccgg tgcgcctgcc ggacaaccac tatctgagca cccagaccac gctgagcaaa
     1441 gacccgaacg agaagcgcga tcacatggtc ctgaaggagt tcgtgacggc agccggtatc
     1501 actaaaggtg aagatgaaag tggatccatg tctgaatata ttcgggtaac cgaagatgag
     1561 aacgatgagc ccattgaaat accatcggaa gacgatggga cggtgctgct ctccacggtt
     1621 acagcccagt ttccaggggc gtgtgggctt cgctacagga atccagtgtc tcagtgtatg
     1681 agaggtgtcc ggctggtaga aggaattctg catgccccag atgctggctg gggaaatctg
     1741 gtgtatgttg tcaactatcc aaaagataac aaaagaaaaa tggatgagac agatgcttca
     1801 tcagcagtga aagtgaaaag agcagtccag aaaacatccg atttaatagt gttgggtctc
     1861 ccatggaaaa caaccgaaca ggacctgaaa gagtatttta gtacctttgg agaagttctt
     1921 atggtgcagg tcaagaaaga tcttaagact ggtcattcaa aggggtttgg ctttgttcgt
     1981 tttacggaat atgaaacaca agtgaaagta atgtcacagc gacatatgat agatggacga
     2041 tggtgtgact gcaaacttcc taattctaag caaagccaag atgagccttt gagaagcaga
     2101 aaagtgtttg tggggcgctg tacagaggac atgactgagg atgagctgcg ggagttcttc
     2161 tctcagtacg gggatgtgat ggatgtcttc atccccaagc cattcagggc ctttgccttt
     2221 gttacatttg cagatgatca gattgcgcag tctctttgtg gagaggactt gatcattaaa
     2281 ggaatcagcg ttcatatatc caatgccgaa ccttagacta gacccagctt tccaatgccg
     2341 aaccttagac tagacccagc tttcttgtac aaagtggttg atcctctatc cagcacagtg
     2401 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2461 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2521 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2581 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2641 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2701 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2761 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     2821 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2881 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2941 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3001 gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     3061 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     3121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3241 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     3361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     3421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     3481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
     3541 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3601 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3661 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3721 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3781 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3841 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3901 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3961 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4021 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4081 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     4141 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc
     4201 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4261 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     4321 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4381 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4441 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4501 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4561 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4621 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4681 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4801 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4861 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4921 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4981 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5041 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5101 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5161 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5221 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5281 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5341 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5401 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5461 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5521 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7501 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7561 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7621 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7681 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7741 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7801 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7861 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7921 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7981 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8161 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8221 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8281 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8341 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8401 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8461 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8521 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8581 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8641 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8701 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8761 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8821 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8881 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8941 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9001 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9061 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9121 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9181 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9241 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9301 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9361 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9421 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     9481 agaagaagaa ggtggagaga gagacaga
//
