LOCUS       sequence-231667-        9113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-3-VEGFR3
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4887..4904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4893..5117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5163..5591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5250..5269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5460..5481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5600..5620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5605..5934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5785..5920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5847..5866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5982..6029
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6048..6422
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6552..6673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6589..6608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6643..6662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6722..6738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6722..6738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6735..6757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6746..6762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6770..6776,6777..6794,6795..6800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6815..6836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6953..6970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7124..7712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7204..7223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7883..8674,8675..8743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8506..8525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8744..8848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8923..8942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgcatgcca ccatgcagcg gggcgccgcg ctgtgcctgc
      721 gactgtggct ctgcctggga ctcctggacg gcctggtgag tggctactcc atgacccccc
      781 cgaccttgaa catcacggag gagtcacacg tcatcgacac cggtgacagc ctgtccatct
      841 cctgcagggg acagcacccc ctcgagtggg cttggccagg agctcaggag gcgccagcca
      901 ccggagacaa ggacagcgag gacacggggg tggtgcgaga ctgcgagggc acagacgcca
      961 ggccctactg caaggtgttg ctgctgcacg aggtacatgc caacgacaca ggcagctacg
     1021 tctgctacta caagtacatc aaggcacgca tcgagggcac cacggccgcc agctcctacg
     1081 tgttcgtgag agactttgag cagccattca tcaacaagcc tgacacgctc ttggtcaaca
     1141 ggaaggacgc catgtgggtg ccctgtctgg tgtccatccc cggcctcaat gtcacgctgc
     1201 gctcgcaaag ctcggtgctg tggccagacg ggcaggaggt ggtgtgggat gaccggcggg
     1261 gcatgctcgt gtccacgcca ctgctgcacg atgccctgta cctgcagtgc gagaccacct
     1321 ggggagacca ggacttcctt tccaacccct tcctggtgca catcacaggc aacgagctct
     1381 atgacatcca gctgttgccc aggaagtcgc tggagctgct ggtaggggag aagctggtcc
     1441 tgaactgcac cgtgtgggct gagtttaact caggtgtcac ctttgactgg gactacccag
     1501 ggaagcaggc agagcggggt aagtgggtgc ccgagcgacg ctcccagcag acccacacag
     1561 aactctccag catcctgacc atccacaacg tcagccagca cgacctgggc tcgtatgtgt
     1621 gcaaggccaa caacggcatc cagcgatttc gggagagcac cgaggtcatt gtgcatgaaa
     1681 atcccttcat cagcgtcgag tggctcaaag gacccatcct ggaggccacg gcaggagacg
     1741 agctggtgaa gctgcccgtg aagctggcag cgtacccccc gcccgagttc cagtggtaca
     1801 aggatggaaa ggcactgtcc gggcgccaca gtccacatgc cctggtgctc aaggaggtga
     1861 cagaggccag cacaggcacc tacaccctcg ccctgtggaa ctccgctgct ggcctgaggc
     1921 gcaacatcag cctggagctg gtggtgaatg tgccccccca gatacatgag aaggaggcct
     1981 cctcccccag catctactcg cgtcacagcc gccaggccct cacctgcacg gcctacgggg
     2041 tgcccctgcc tctcagcatc cagtggcact ggcggccctg gacaccctgc aagatgtttg
     2101 cccagcgtag tctccggcgg cggcagcagc aagacctcat gccacagtgc cgtgactgga
     2161 gggcggtgac cacgcaggat gccgtgaacc ccatcgagag cctggacacc tggaccgagt
     2221 ttgtggaggg aaagaataag actgtgagca agctggtgat ccagaatgcc aacgtgtctg
     2281 ccatgtacaa gtgtgtggtc tccaacaagg tgggccagga tgagcggctc atctacttct
     2341 atgtgaccac catccccgac ggcttcacca tcgaatccaa gccatccgag gagctactag
     2401 agggccagcc ggtgctcctg agctgccaag ccgacagcta caagtacgag catctgcgct
     2461 ggtaccgcct caacctgtcc acgctgcacg atgcgcacgg gaacccgctt ctgctcgact
     2521 gcaagaacgt gcatctgttc gccacccctc tggccgccag cctggaggag gtggcacctg
     2581 gggcgcgcca cgccacgctc agcctgagta tcccccgcgt cgcgcccgag cacgagggcc
     2641 actatgtgtg cgaagtgcaa gaccggcgca gccatgacaa gcactgccac aagaagtacc
     2701 tgtcggtgca ggccctggaa gcccctcggc tcacgcagaa cttgaccgac ctcctggtga
     2761 acgtgagcga ctcgctggag atgcagtgct tggtggccgg agcgcacgcg cccagcatcg
     2821 tgtggtacaa agacgagagg ctgctggagg aaaagtctgg agtcgacttg gcggactcca
     2881 accagaagct gagcatccag cgcgtgcgcg aggaggatgc gggacgctat ctgtgcagcg
     2941 tgtgcaacgc caagggctgc gtcaactcct ccgccagcgt ggccgtggaa ggctccgagg
     3001 ataagggcag catggagatc gtgatccttg tcggtaccgg cgtcatcgct gtcttcttct
     3061 gggtcctcct cctcctcatc ttctgtaaca tgaggaggcc ggcccacgca gacatcaaga
     3121 cgggctacct gtccatcatc atggaccccg gggaggtgcc tctggaggag caatgcgaat
     3181 acctgtccta cgatgccagc cagtgggaat tcccccgaga gcggctgcac ctggggagag
     3241 tgctcggcta cggcgccttc gggaaggtgg tggaagcctc cgctttcggc atccacaagg
     3301 gcagcagctg tgacaccgtg gccgtgaaaa tgctgaaaga gggcgccacg gccagcgagc
     3361 accgcgcgct gatgtcggag ctcaagatcc tcattcacat cggcaaccac ctcaacgtgg
     3421 tcaacctcct cggggcgtgc accaagccgc agggccccct catggtgatc gtggagttct
     3481 gcaagtacgg caacctctcc aacttcctgc gcgccaagcg ggacgccttc agcccctgcg
     3541 cggagaagtc tcccgagcag cgcggacgct tccgcgccat ggtggagctc gccaggctgg
     3601 atcggaggcg gccggggagc agcgacaggg tcctcttcgc gcggttctcg aagaccgagg
     3661 gcggagcgag gcgggcttct ccagaccaag aagctgagga cctgtggctg agcccgctga
     3721 ccatggaaga tcttgtctgc tacagcttcc aggtggccag agggatggag ttcctggctt
     3781 cccgaaagtg catccacaga gacctggctg ctcggaacat tctgctgtcg gaaagcgacg
     3841 tggtgaagat ctgtgacttt ggccttgccc gggacatcta caaagaccct gactacgtcc
     3901 gcaagggcag tgcccggctg cccctgaagt ggatggcccc tgaaagcatc ttcgacaagg
     3961 tgtacaccac gcagagtgac gtgtggtcct ttggggtgct tctctgggag atcttctctc
     4021 tgggggcctc cccgtaccct ggggtgcaga tcaatgagga gttctgccag cggctgagag
     4081 acggcacaag gatgagggcc ccggagctgg ccactcccgc catacgccgc atcatgctga
     4141 actgctggtc cggagacccc aaggcgagac ctgcattctc ggagctggtg gagatcctgg
     4201 gggacctgct ccagggcagg ggcctgcaag aggaagagga ggtctgcatg gccccgcgca
     4261 gctctcagag ctcagaagag ggcagcttct cgcaggtgtc caccatggcc ctacacatcg
     4321 cccaggctga cgctgaggac agcccgccaa gcctgcagcg ccacagcctg gccgccaggt
     4381 attacaactg ggtgtccttt cccgggtgcc tggccagagg ggctgagacc cgtggttcct
     4441 ccaggatgaa gacatttgag gaattcccca tgaccccaac gacctacaaa ggctctgtgg
     4501 acaaccagac agacagtggg atggtgctgg cctcggagga gtttgagcag atagagagca
     4561 ggcatagaca agaaagcggc ttcagctgta aaggacctgg ccagaatgtg gctgtgacca
     4621 gggcacaccc tgactcccaa gggaggcggc ggcggcctga gcggggggcc cgaggaggcc
     4681 aggtgtttta caacagcgag tatggggagc tgtcggagcc aagcgaggag gaccactgct
     4741 ccccgtctgc ccgcgtgact ttcttcacag acaacagcta ctaagcagca tcgggatcca
     4801 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag
     4861 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4921 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4981 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5041 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5101 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5161 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5581 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5701 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5761 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5941 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     6001 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     6061 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     6121 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     6181 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     6241 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     6301 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     6361 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     6421 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6481 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6541 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6601 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6661 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8881 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     8941 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9001 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     9061 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
