LOCUS       sequence-231669-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-MTMR10-201
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..525
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(61..84)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     458..477
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      566..575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             572..2224
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4910..5031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4921..4940)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4975..4994
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5276..5633
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5276..5467
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5299..5319)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5484..5619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5678..5687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5684..6616
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6668..7459
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6719..6738)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7329..7348
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7523..7571
                     /note="synthetic polyadenylation signal"
     promoter        7727..7831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7832..7900,7901..8692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8050..8069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8850..9438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8930..8949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9554..9837
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ccggcgtaga ggatcgagat ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
       61 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
      121 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
      181 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
      241 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
      301 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      361 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
      421 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
      481 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
      541 ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg
      601 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
      661 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
      721 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
      781 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
      841 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
      901 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
      961 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     1021 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     1081 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     1141 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     1201 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     1261 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     1321 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     1381 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     1441 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     1501 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     1561 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     1621 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     1681 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     1741 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
     1801 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     1861 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     1921 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     1981 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     2041 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     2101 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     2161 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     2221 gtaattctag ttgtttaaac gagctcgaat agggtgtttt ctgaacattt tgagggaagc
     2281 tgtcaacttt tttcctctga attaacattg ctaacctagg cgtttgaatc tctaataact
     2341 ttatatgtaa gaataatagt tggaatttgc actaatattt aaaaacatgt tcaatcatgc
     2401 ttctttcaca cttattttaa gagagatgta aattttgttc ctgtcctctt tctgtcatta
     2461 caggtctggc tcttgtaacc gtgatcaaac tgttcatgtt gtctgctaca tttttgtctc
     2521 catccatttt tcctaccacc tcctgaaggc tatctgatag tcagtcacat tagcagcccc
     2581 aggcagcaga caacaggaaa gttaggaaat ttgtgtttcg tgtcattttt aggagcatct
     2641 gataaaacct ccagcaggtt ttaggaagta ttcatgtatt tttctggtta ctttctgtca
     2701 tctctaattg aactcacctg atgaaggttc agtgttctgg ggccagaatt tatgatttta
     2761 gatcaccttc tttggaacct tagatcactg tgttttgaaa tcatgagttt gcttttaact
     2821 tcatagggtc aactttaaaa tgatatgcac tgttaatttt aaagcatttg ctgcagataa
     2881 ttaaacttag aagtgccttt gactttagga tacaaatatt acagaagaaa atataatttc
     2941 actttttaaa attggggtgg gaaaatccca ttgcatattt gaaataggct tttcatacta
     3001 agcttcatag ccaggagtcc ccagagtctt gttcctctga aagccactgg ggagtggcct
     3061 ctggggtgct gattccacag aggtgtatgc tgtagacagg agagtgccat ctatgccaaa
     3121 actcgccctc aaaaacaaac aaggcttgct gggaggcgtg ctgggcttgg ccatcagtat
     3181 ttccagtgtg gtaaactatt gctggcactt ccccctggaa ataactaatg aggttacgag
     3241 ttgggcacct gcacagatgt ccttctctca tagttcctaa tgcttaggaa tagaggagaa
     3301 ataaaaaaat ggattctctc aaaacactgc catttgaata gcgacagaag tgctccccca
     3361 gcccccaact ttggacagca aagttgagga gaatgagcag acacagttgt ttgcttgatc
     3421 tgaatctctc taaagtaaag tatttccaaa ctgtgtgaca agagcctacc taccactgta
     3481 gcggtcaaag ctgaagcttc ttacagcagt gaaacggggc accacctccc ccacactcct
     3541 cattccccgc ttaaaacatg gatactttca aatttgactg tttcttaaac tgccatccta
     3601 agatatggaa aatttttata gtaaagtgtc tagttagctt atttcctttt ctaaaacaag
     3661 tgttttcaag ataactgtat tttaccttta tatgtactga atagctgttt ctttttgaat
     3721 tatttgcctt ttaaaatttg ataatgtctc tggatataac aggacaggag ttcttaaaaa
     3781 atatcttaag aaattcactt tatgggtaaa cccaaggttt ttgccaactt gttgcctaga
     3841 aaataagggc tagtttcagt ttatacaaat agaattatta aacattttac agtccttgat
     3901 tagaaaccag acccaatctc cttataacac cacagcgtat cctgccattg acagtgtaat
     3961 cacaattctc cctttttcat ttagctgctt ttttattatt actaaatgtt ttggattgag
     4021 catttttccc tctgtaattt tcttccttca cgtttatttt attttaactc ttgtagtatt
     4081 ttattgttgt taatttacaa gtttaaaaat attaggtact attaataatg gttaaaaata
     4141 gaaaaatgca tatttttgta tgataatcaa atgtaaaata cttttatttt tgctggacag
     4201 ttgttatatc atgattattg tgctacagtt tattgtgcat aatatgaaaa acaactatga
     4261 cagccttcag tcgggccagg gtgaagctgc ttataccacc tctgccgtca gagggacatg
     4321 tggtgacagc agtggtgtgg ctgcacaggg cgcactagag agagctcagc acccctgctg
     4381 cccgccagca gagcccgtgc tgagggaatg ccgcacagat gctgatgcac tgggtgaaat
     4441 ttctagtatt gaacgtaaag gtgtacagtg tcttgctgtt attttatgat ggaaactgat
     4501 tttgaaacca aaaatagcta actaacttta tttaaggaaa ggatattaat ttgtactaac
     4561 agagggtgaa agctgttcac atttgtcaac aaaatctgct tgctgcagta gtaacctcaa
     4621 gtggttaaaa cttgatttcc cgagaaaact aaaacctttg tgcctaaaac tgatgacttg
     4681 agttcaagtg ggatgagcaa gaagatgtgt tatcttgttg ttcaacagta ttgagtgtga
     4741 aggaaatttt gatggcttaa taaaattcca cagcgactgt tagtcgacct gcaggcatgc
     4801 aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     4861 agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat aagatacatt
     4921 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     4981 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     5041 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag
     5101 taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc ttacaatttc
     5161 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct
     5221 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
     5281 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5341 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5401 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5461 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5521 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5581 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     5641 attcttctga cacaacagtc tcgaaccaaa ggctggagcc accatggctt ccaaggtgta
     5701 cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca
     5761 aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc
     5821 cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca
     5881 catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa
     5941 gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga
     6001 gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc
     6061 ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt
     6121 cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat
     6181 caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct
     6241 cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt
     6301 caaggagaag ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt
     6361 taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc
     6421 cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat
     6481 tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt
     6541 cagccaggag gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt
     6601 gctgaagaac gagcagaccg gtggtgggag cggaggtggc ggatcaggtg gcggaggctc
     6661 cggagggatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6721 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6781 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6841 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6901 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     6961 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7021 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7081 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7141 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     7201 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7261 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7321 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7381 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7441 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     7501 aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     7561 ttttttgtgt gaatcgatag cgataaggat cctctttgcg cttgcgtttt cccttgtcca
     7621 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     7681 cgtaatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7741 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7801 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7861 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7921 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7981 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8041 caaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8101 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8161 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8221 cactgcggcc aacttacttc tgacaactat cggaggaccg aaggagctaa ccgctttttt
     8281 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8341 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8401 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8461 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8521 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8581 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8641 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aattcgaaat
     8701 gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc
     8761 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8821 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8881 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8941 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9001 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9061 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9121 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9181 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9241 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9301 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9361 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9421 agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9481 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9541 atccttttat agtccggaaa tacaggaacg cacgctggat ggcccttcgc tgggatggtg
     9601 aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc tgtaccctct
     9661 ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gattgcggca gtttttcggg
     9721 tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt
     9781 gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag ccatcgagat
     9841 aaaccgcagt actccggcca cgatgcgt
//
