LOCUS       sequence-231672-       12659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 12659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-CRTAP-201
FEATURES             Location/Qualifiers
     source          1..12659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     276..295
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             390..2042
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    7519..7640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7530..7549)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7584..7603
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7885..8242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7885..8076
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7908..7928)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8093..8228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      8287..8296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8293..9225
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             9277..10068
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9328..9347)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9938..9957
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10132..10180
                     /note="synthetic polyadenylation signal"
     promoter        10336..10440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10441..10509,10510..11301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10659..10678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(11459..12047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11539..11558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    12163..12446
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(12503..12659,1..343)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12538..12561)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
       61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      361 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
      421 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      481 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      541 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      601 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      661 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      721 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
      781 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
      841 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
      901 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
      961 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1021 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1081 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1141 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1201 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1261 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1321 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1381 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1441 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1501 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1561 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1621 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1681 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1741 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1801 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     1861 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     1921 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     1981 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2041 aattctagtt gtttaaacga gctcgcccac agcaaccaaa gagacttcct cttggcgttc
     2101 aggaaacaca gattctttgt ccttttccca acagcccagg ctgttgatac ctcagagcct
     2161 tctctttact ctccaaagtg aaagggaagc ccccgtctct ctaactgcat gtcatcaggg
     2221 gtgagcctgc ctttcctatc ttcacacctg ccacctcatg ttcacaccta tctttctcac
     2281 cttttttttg agatggagtc tcgctctctt gcccaggctg gagtgcaatg gcacgttctc
     2341 agctcactgc aacctccgcc tcttgggttc aagcaattct gctgcatcag cctcccgagt
     2401 acctgggatt acaggcatgt gccaccacgc ccggctaatt ttgtattttt agtagagacg
     2461 gggttttgcc atgttggcca ggctggtctc gaactcttga cttcagatga tccatctgcc
     2521 ttggcctccc acagtgctgg gattacaggc gtgagccacc atgcccggcc tctttctcac
     2581 ctttacacct gtcttcttat cctcacatct gttttcacac cttcatccct gtcttcctca
     2641 tgttcacact tgtcttcccc atgttcatag ctgcctttct taccattttg gtttgaaggg
     2701 cagtcttctc tggcttgttt ttttgttttt cccagaaaat cagtattatt ttttaaataa
     2761 gaaaaacatt cctagaagat gataattgtg aaaacctcct ttggcttatt tgcttttcca
     2821 gattttagtc tcctttctcc ccatccggga aagatggtgg aagacatagg ctaaatttct
     2881 ccagcctcac aatggtcttc acttggtctg acttgtacca attctagcac ccactgaaaa
     2941 acaagttgag tagagagtgt agagtgcaga aatgtggctt ttgccccact ttgcatctcc
     3001 aaaattacaa cggttggccg atcccatttg aggacaatgc ttagttataa gtctccgagt
     3061 tggaaaagga agaaagccag agctgtctag tttcattcat tctttcagta aatatttatt
     3121 gagtacctac tgtgtgctag gcattgacct gggaactaga gatacttcac agaataacag
     3181 ggaaagttcc ctgtgctcat ggagcttaca ttctacaggg agaaagagat agccaataca
     3241 taggaataaa tatatacaag gtatcatgta gtgataattg ctgtggagaa aaataaagca
     3301 ggggagggag taagaaatcc tggagatgag gctgcagttt taaatggggc ctcactggga
     3361 atgtgacgtt gagcagagac gttagggaag tggatcctgg acaaggcatt ccaggcagag
     3421 gaacaggatg tgcactgccc caaagtgaga acttgctcta cgtggtcagg aaagagcagg
     3481 gagaccaagc agagtcgtgg gcaggggtag aatggaagga gaggcggctg gggaggacag
     3541 gtggtggagg gccttggctt ctgctaagtg agatgggaac cactggaggg tttgaacaga
     3601 ggagtgcctt gattgattta tattttgcaa gggtcattct agctgcaata ttgtgaaaaa
     3661 ctttagtgga caagggcaga aggaagaggg aagacctgtt aggaagctac tgcaaggttc
     3721 caggcttggg cctgggccac agcaacagca gtggtcaaat atctagattt attttgaaaa
     3781 gagccaatag gatttgctga gagtttgaat gtggagtgta agagaaggaa gagttaatga
     3841 tgacattaag gtttttggcc tgaatagcag gaaagatgga gttaccagtt actgaaatag
     3901 ggaaggatgg gctgggtaag taaggaattt ggtgcaaagc aggctgtctg tggttggaat
     3961 gggaggttct ggctgcaaat caaagtggag agttctctca ggtcaggtct gcagcagagc
     4021 tcgagacagg gatctgaatg cacttggttt attgttgggg gtgctctcag aaggaacctg
     4081 tgaaagcctt tatcagtcat ttattggctg tgagaagttc tctgggagtg tgggtacatt
     4141 tgaaggcaag tgacttcagt tgagggcaag tctctggaaa agaggctgta ggcatctggc
     4201 agctaccatg cgtggtagtg tgttgggggt gggggtcctg ggcactggct gtgtgaaggg
     4261 atctggcagg gcaccacagc gccccctact gaaccatcag catgtcagtg gcatttaaag
     4321 ccatgcagct ggaggggcca ctgagattgt ctctgagtat tactgagaag caacagaaaa
     4381 gagccatgga tggagccctt gggctctctg ggaaatggga aatcagccaa aggactgaga
     4441 aggagttacc ttaaggtcag agaaaaccaa gagagtgtgg tgttctggaa gctgagcttt
     4501 ctttattcaa cctcattccc ttctccaaat aagccacttg tgtagttggg cccctccagg
     4561 gttgaaggca agaggagaaa ggcacagcgt ttgggaaaca agacttttcc tgcaatagcc
     4621 tgggaaggaa taaaaggata gagtgtttgg gtttttgtgt aatggtggtt aattggggtg
     4681 gaacactcac acgttgtgct ttttctgggc ttcccttatc ccccagaaca ctctaccaac
     4741 ctcggggaac tcgggcacat ccttctgttt ctccttcagc tctatcctgc tttcctcatc
     4801 ccttctgaca ccacgtcctc actcacctgc acaagaatcc ctgcatcagg ttctcctttg
     4861 agggtaccca cccaggacag tcccctacca cttctgtctt gggctgaagt tgcccacgtc
     4921 cacaaaatct gtactcccag cgggggtgtt tggcccgagg agtcagtgtt attactggtg
     4981 gatgcaccgt gtccacagca gcccccaatc ccagcgatgc gtcagatctt acgtggcttc
     5041 ctgctggggg agatggcctt cacccacggg atgccgggtt ctcctttctt tcctcacccc
     5101 aacctttact ccaccagaga aacttccttt tgaactcagt ggggaagagg gtgatgagac
     5161 aggactagaa agtagtgggg gacccagcga gtggacgccc tgctccagga ttcctgagtc
     5221 tgtaaatagt gtgcccagca gctgtgaact ccccctatag cctcaggctg cagtgtcctt
     5281 cccagctgtg tgagaaaatg aaagccgacg tccacaggga cccaggcagg gttgggtgtt
     5341 gtgactcact ccacctctgt gccctgcaga ggtactgttg ggtccttgtc ttgtgagcct
     5401 ggggtgagct ctctgtacat gttgttgttc cacgtatggg ttgacttggc atgctggggg
     5461 gtcctcgttc actctctgaa gttggcctcc tttcactggg gattgaaaag cacctccacc
     5521 cctaccctag tgatgtcccc tgaggacccg ggtgatagta cagtcaatat tgtcagtact
     5581 ttgctttgat tgaaggctgt agagctgagt taccaaaatt tctatttcaa aggaaaccaa
     5641 accttaaaaa aaaaaaacaa aaactgggct gggtcttcca aacctaccat gaaaccctgg
     5701 tgtgcaggct gcactcaatg acctcaaccc aacacctccc tgagtgtgct tcttggaaga
     5761 gcctagaaga ttcctggatg gagaccccat tggttcagcc tcaagtctgg cccgtcttcg
     5821 aaaaaacaaa cacatttgta agctttgtgg gagcttccag gcctgctcta agatgccttg
     5881 cttgtccttt gacccatcag catggagctc agtggttgct gtttggttct gcaggctggt
     5941 ggggaggccg cccatcgtgg tggggcatct gtccagcccc attgccactc agggcatcca
     6001 aacaggaggc acccgctggg aagggtctaa agatactcct tgtggccact gctactgttc
     6061 acacttgact tgtggagaag cgaagggctg aggggaggtt tgtgtacacc catgtattta
     6121 aaagtgactg actgactgaa atgagcacat accgacatat gcaacatact aataccttcc
     6181 tgattttcga gactttctaa ttactacaac taacctgttg tgctcacctc tggaattcag
     6241 aaagagagcc actgcgagca ctgaccacaa gggctgcctt aggaaggaaa tgtgtgcttt
     6301 caggagttct tattagggga attttaagag cacaaaaatt ttattgaacc caccttagtg
     6361 acaaacagaa aatgatttgt tagattgtca gttggaaggg gtttattatg actgctggtg
     6421 aaataaacag tgaccagttt ctgccaacat ttatggaaat aacgtttcta ggttttaaat
     6481 gtgagccgta aactgaagct acttcagtta aaaaaaaaaa aatcaatact taaactgtag
     6541 ggaaaaggtg gattggtgcc agagaaaaca ttatttaata gtgtcagaac atggaactgc
     6601 aacacagttt gtagcaaggc actgagaaaa acccacaact aatccttcat cgcagctgtc
     6661 tgaactcttg atcatctgcc atcccccaaa cagtgacttc tttttttctg gtgactccag
     6721 gcctgaatga cctagtgtgg agagtttaaa ctatgtacaa gggaggaaag aaaaaaagga
     6781 aaggaacctt aagtaagcct cagccaaagg ttttatgcat tggacttcct gtgcctgccc
     6841 ccaggggcaa gactgatccc catgcctgtg cccatgacat ctccctgaaa gaggacacca
     6901 tgacagcccg gctttgcctt gactgaccca ctgctacccc agaaataaga atcaagagca
     6961 gctattgtta tccttagagt gttttccgtc tagggccggc tcgtgaacag ccacatatcc
     7021 ttgcacctga cactgtcccc acacaaatag ctggctttcg ttgcttgttg aatgaatgag
     7081 tgagttggct ctatatcccc ttggagctgg ccggtaagat attagtgcct cattttacaa
     7141 gaagagaata ggaaggaatt aagcaatttg gccaacagat acaagataga ttccagagtt
     7201 ttatctccca ctttagggtg gcagccagta ggccaaactc caaagaccgt tgctgatgtc
     7261 tttttctgcc tcccctcttt gggttagtgt ggtatgtaca agctcactct tgttgaaaat
     7321 tagaaaatag ttgaaaacaa aaggtttttg tttttctttt taaatcacat taaatgtttt
     7381 acattgctta agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa
     7441 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc
     7501 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7561 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7621 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     7681 gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat
     7741 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     7801 gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga
     7861 aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca
     7921 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7981 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8041 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8101 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8161 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8221 tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaaccaaag
     8281 gctggagcca ccatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact
     8341 gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac
     8401 tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc
     8461 agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct
     8521 gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat
     8581 cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt
     8641 gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag
     8701 atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg
     8761 cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt
     8821 gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct
     8881 gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc
     8941 ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt
     9001 gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag
     9061 tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc
     9121 gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt
     9181 aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagaccgg tggtgggagc
     9241 ggaggtggcg gatcaggtgg cggaggctcc ggagggattg aacaagatgg attgcacgca
     9301 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     9361 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     9421 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     9481 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     9541 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     9601 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     9661 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     9721 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     9781 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     9841 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     9901 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     9961 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
    10021 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
    10081 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
    10141 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
    10201 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
    10261 cagcaccgtt tctgcggact ggctttctac gtaatggttt cttagacgtc aggtggcact
    10321 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    10381 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    10441 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    10501 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    10561 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    10621 gaagaacgtt ttccaatgat gagcactttc aaagttctgc tatgtggcgc ggtattatcc
    10681 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    10741 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    10801 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaactatc
    10861 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    10921 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    10981 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    11041 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    11101 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    11161 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    11221 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    11281 tcactgatta agcattggta attcgaaatg accgaccaag cgacgcccaa ccggtatcag
    11341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11881 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    12001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atttcaagaa gatcctttga
    12061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    12121 tgagattatc aaaaaggatc ttcacctaga tccttttata gtccggaaat acaggaacgc
    12181 acgctggatg gcccttcgct gggatggtga aaccatgaaa aatggcagct tcagtggatt
    12241 aagtgggggt aatgtggcct gtaccctctg gttgcatagg tattcatacg gttaaaattt
    12301 atcaggcgcg attgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc
    12361 cgtgatcgcg ctgaacgcgt tttagcggtg cgtacaatta agggattatg gtaaatccac
    12421 ttactgtctg ccctcgtagc catcgagata aaccgcagta ctccggccac gatgcgtccg
    12481 gcgtagagga tcgagatcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    12541 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    12601 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcc
//
