LOCUS       sequence-231681-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-TNFRSF10B
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..539
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(75..98)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     472..491
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             586..2238
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4928..5049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4939..4958)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4993..5012
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5294..5651
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5294..5485
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5317..5337)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5502..5637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5696..5705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5702..6634
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6686..7477
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6737..6756)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7347..7366
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7541..7589
                     /note="synthetic polyadenylation signal"
     promoter        7745..7849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7850..7918,7919..8710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8068..8087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8868..9456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8948..8967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9572..9855
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctaccg ggtaggggag gcgcttttcc
       61 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
      121 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
      181 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
      241 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
      301 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
      361 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
      421 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
      481 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
      541 cctgcagccc aagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa
      601 aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag
      661 ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca
      721 catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa
      781 gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc
      841 ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct
      901 aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc
      961 gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc
     1021 atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac
     1081 accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc
     1141 ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc
     1201 aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc
     1261 atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac
     1321 ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg
     1381 taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc
     1441 ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac
     1501 ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag
     1561 gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca
     1621 accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg
     1681 gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac
     1741 cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc
     1801 gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac
     1861 tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag
     1921 ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc
     1981 gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc
     2041 gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag
     2101 gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga
     2161 ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc
     2221 ggcaagatcg ccgtgtaatt ctagttgttt aaacgagctc ggtgtgattc tcttcaggaa
     2281 gtcagacctt ccctggttta ccttttttct ggaaaaagcc caactggact ccagtcagta
     2341 ggaaagtgcc acaattgtca catgaccggt actggaagaa actctcccat ccaacatcac
     2401 ccagtggatg gaacatcctg taacttttca ctgcacttgg cattattttt ataagctgaa
     2461 tgtgataata aggacactat ggaaatgtct ggatcattcc gtttgtgcgt actttgagat
     2521 ttggtttggg atgtcattgt tttcacagca cttttttatc ctaatgtaaa tgctttattt
     2581 atttatttgg gctacattgt aagatccatc tacacagtcg ttgtccgact tcacttgata
     2641 ctatatgata tgaacctttt ttgggtgggg ggtgcggggc agttcactct gtctcccagg
     2701 ctggagtgca atggtgcaat cttggctcac tatagccttg acctctcagg ctcaagcgat
     2761 tctcccacct cagccatcca aatagctggg accacaggtg tgcaccacca cgcccggcta
     2821 attttttgta ttttgtctag atataggggc tctctatgtt gctcagggtg gtctcgaatt
     2881 cctggactca agcagtctgc ccacctcaga ctcccaaagc ggtggaatta gaggcgtgag
     2941 cccccatgct tggccttacc tttctacttt tataattctg tatgttatta ttttatgaac
     3001 atgaagaaac tttagtaaat gtacttgttt acatagttat gtgaatagat tagataaaca
     3061 taaaaggagg agacatacaa tgggggaaga agaagaagtc ccctgtaaga tgtcactgtc
     3121 tgggttccag ccctccctca gatgtacttt ggcttcaatg attggcaact tctacagggg
     3181 ccagtctttt gaactggaca accttacaag tatatgagta ttatttatag gtagttgttt
     3241 acatatgagt cgggaccaaa gagaactgga tccacgtgaa gtcctgtgtg tggctggtcc
     3301 ctacctgggc agtctcattt gcacccatag cccccatcta tggacaggct gggacagagg
     3361 cagatgggtt agatcacaca taacaatagg gtctatgtca tatcccaagt gaacttgagc
     3421 cctgtttggg ctcaggagat agaagacaaa atctgtctcc cacgtctgcc atggcatcaa
     3481 gggggaagag tagatggtgc ttgagaatgg tgtgaaatgg ttgccatctc aggagtagat
     3541 ggcccggctc acttctggtt atctgtcacc ctgagcccat gagctgcctt ttagggtaca
     3601 gattgcctac ttgaggacct tggccgctct gtaagcatct gactcatctc agaaatgtca
     3661 attcttaaac actgtggcaa caggacctag aatggctgac gcattaaggt tttcttcttg
     3721 tgtcctgttc tattattgtt ttaagacctc agtaaccatt tcagcctctt tccagcaaac
     3781 ccttctccat agtatttcag tcatggaagg atcatttatg caggtagtca ttccaggagt
     3841 ttttggtctt ttctgtctca aggcattgtg tgttttgttc cgggactggt ttgggtggga
     3901 caaagttaga attgcctgaa gatcacacat tcagactgtt gtgtctgtgg agttttagga
     3961 gtggggggtg acctttctgg tctttgcact tccatcctct cccacttcca tctggcatcc
     4021 cacgcgttgt cccctgcact tctggaaggc acagggtgct gctgcctcct ggtctttgcc
     4081 tttgctgggc cttctgtgca ggacgctcag cctcagggct cagaaggtgc cagtccggtc
     4141 ccaggtccct tgtcccttcc acagaggcct tcctagaaga tgcatctaga gtgtcagcct
     4201 tatcagtgtt taagattttt cttttatttt taattttttt gagacagaat ctcactctct
     4261 cgcccaggct ggagtgcaac ggtacgatct tggctcagtg caacctccgc ctcctgggtt
     4321 caagcgattc tcgtgcctca gcctccggag tagctgggat tgcaggcacc cgccaccacg
     4381 cctggctaat ttttgtattt ttagtagaga cggggtttca ccatgttggt caggctggtc
     4441 tcgaactcct gacctcaggt gatccacctt ggcctccgaa agtgctggga ttacaggcgt
     4501 gagccaccag ccaggccaag ctattctttt aaagtaagct tcctgacgac atgaaataat
     4561 tgggggtttt gttgtttagt tacattaggc tttgctatat ccccaggcca aatagcatgt
     4621 gacacaggac agccatagta tagtgtgtca ctcgtggttg gtgtcctttc atgcttctgc
     4681 cctgtcaaag gtccctattt gaaatgtgtt ataatacaaa caaggaagca cattgtgtac
     4741 aaaatactta tgtatttatg aatccatgac caaattaaat atgaaacctt atataaaaaa
     4801 gtcgacctgc aggcatgcaa gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
     4861 tggctgctgc caccgctgag caataactag cataacccct tggggcggcc gcttcgagca
     4921 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4981 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     5041 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     5101 gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcgaatt ttaacaaaat
     5161 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5221 caccgcatac gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     5281 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5401 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5461 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5581 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5641 gcttttgcaa aaagcttgat tcttctgaca caacagtctc gaaccaaagg ctggagccac
     5701 catggcttcc aaggtgtacg accccgagca acgcaaacgc atgatcactg ggcctcagtg
     5761 gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact atgattccga
     5821 gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctgcctcca gctacctgtg
     5881 gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg atctgatcgg
     5941 aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc actacaagta
     6001 cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg tgggccacga
     6061 ctggggggct tgtctggcct ttcactactc ctacgagcac caagacaaga tcaaggccat
     6121 cgtccatgct gagagtgtcg tggacgtgat cgagtcctgg gacgagtggc ctgacatcga
     6181 ggaggatatc gccctgatca agagcgaaga gggcgagaaa atggtgcttg agaataactt
     6241 cttcgtcgag accatgctcc caagcaagat catgcggaaa ctggagcctg aggagttcgc
     6301 tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc tctcctggcc
     6361 tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg tccgcaacta
     6421 caacgcctac cttcgggcca gcgacgatct gcctaagatg ttcatcgagt ccgaccctgg
     6481 gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg agttcgtgaa
     6541 ggtgaagggc ctccacttca gccaggagga cgctccagat gaaatgggta agtacatcaa
     6601 gagcttcgtg gagcgcgtgc tgaagaacga gcagaccggt ggtgggagcg gaggtggcgg
     6661 atcaggtggc ggaggctccg gagggattga acaagatgga ttgcacgcag gttctccggc
     6721 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6781 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6841 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6901 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6961 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7021 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7081 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     7141 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7201 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7261 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     7321 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     7381 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     7441 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     7501 accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc
     7561 attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc tctttgcgct
     7621 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     7681 ctgcggactg gctttctacg taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8041 tccaatgatg agcactttca aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaactatcg gaggaccgaa
     8281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8401 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8461 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8701 gcattggtaa ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag
     8761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9301 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9421 aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg
     9481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9541 aaaaggatct tcacctagat ccttttatag tccggaaata caggaacgca cgctggatgg
     9601 cccttcgctg ggatggtgaa accatgaaaa atggcagctt cagtggatta agtgggggta
     9661 atgtggcctg taccctctgg ttgcataggt attcatacgg ttaaaattta tcaggcgcga
     9721 ttgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     9781 tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg taaatccact tactgtctgc
     9841 cctcgtagcc atcgagataa accgcagtac tc
//
