LOCUS       sequence-231682-       10103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-TMEM63C-201
FEATURES             Location/Qualifiers
     source          1..10103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     325..344
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      433..442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             439..2091
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5012..5133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5023..5042)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5077..5096
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5378..5735
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5378..5569
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5401..5421)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5586..5721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5780..5789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5786..6718
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6770..7561
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6821..6840)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7431..7450
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7625..7673
                     /note="synthetic polyadenylation signal"
     promoter        7829..7933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7934..8002,8003..8794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8152..8171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8952..9540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9032..9051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9656..9939
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(9996..10103,1..392)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10031..10054)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
       61 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
      121 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
      181 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
      241 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
      301 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
      361 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagcttg gcaatccggt
      421 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      481 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      541 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
      601 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
      661 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
      721 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
      781 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
      841 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
      901 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
      961 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1021 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     1081 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     1141 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     1201 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     1261 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     1321 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     1381 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     1441 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     1501 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     1561 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     1621 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     1681 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     1741 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     1801 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     1861 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     1921 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     1981 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     2041 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta attctagttg
     2101 tttaaacgag ctcgccggga cctgaggcct ccactggcga cttgttgagg ggtcagggga
     2161 gggcctggca aggggaggca ggagggtggc ctggacctcc ccactacctc ctgcagactt
     2221 tgagaagccc acagtggaga catccaccac cccagccatg ggccatacgg gggtcctgac
     2281 ctgctgcccg gctggaactg ggggtgctcg gcagtgctga aggagcctgg gaagggatgg
     2341 gaggatacag gcaagcacat gtcttgagag aggtggctgg agccccggca cagagactga
     2401 acgctggggt cccttcctgg gaccaagatg gagaaggtgt tcctaaggga ggagacagaa
     2461 ggaggctgcc gaaggctctg tggggtcatc accactctgc atcagctgcc cttccaagga
     2521 gcttctgctg ctgctcctcc tcccagcccc ggcccattcc tcccctgcag tctgaggagg
     2581 caaaggtatg tgcacggggc acattgacag gacacggagg accacctcat cacagggttc
     2641 cctgcatggg gatctgtaaa gagaaagttt ctgcacccac cagagcaaga gccaactgaa
     2701 agcgtagacc tgagaagagg taactcagcc ccttcctgct cctctgccct catcagatgt
     2761 ccccaggagc agcagggcag aggcccttct ttctattctt acaagggtag ctagagcgtg
     2821 atcactcagg gctcatcaaa tgagactcgt gtgcattttt cagaaggaaa ccttggttag
     2881 tccttgctgg gtaacacaaa gtggggtgag acgacagaag ccgaattcat ggaagggggg
     2941 tcttctcccc aaaactctgt gtggtgggaa accagctata cctccccaag ccccagggcc
     3001 taaagagaag acccccgaag ccaaagatgt ggccacttaa aagcgtctcc tgcctcctac
     3061 ccaactgagt gcctgggccc ccagcttggc caagatgggc agtacgttag ggtaagaacc
     3121 ccatgcttca aacttaagga ctgaccatca cctgcgtccc aagtaggacc cttcctccct
     3181 tctcggggct gcccctgcac cctgccttga agaccaccca agcggcctcc agtgtgggcc
     3241 tggtccagac attgcagatg cttcaaccgt gatgtcgccc caggcctgcc aggggtgtgg
     3301 tggaggggaa ggccacgtgc tccagggaga agccctttct ggagaagcaa ggctgtcctc
     3361 ccagggctgc cactaccaga gacctggggg agctgaattc cgaacagtga tggtgacact
     3421 cagcaccttt gccacagccg gggggaaccg gcttctgcct ctgggatggg ctctcatcag
     3481 gaccaccgtg cagcccagcc agggaggaca tgagaagggc cagtgggggc ctcaatgaac
     3541 cagaacaagc caagctgaat ggggtctgtg tgctccaggg ccctcttcag ccccctcccc
     3601 caaaggtctg ggtccctgcc accaacctac tgaaggccgg cccccggctc acctcacctg
     3661 agcacctgca ccaggcccca ggcacatggc tgccctgaac tcagatcacc tagaccttgt
     3721 ccctgcccca cctttgcccc atcctagccc cagaagctcc aagcttcacc gcaggtgaga
     3781 aattgtgctc aatgggcaga aactgctata cccccagggc atggcccaca ttttggcatg
     3841 agggtgtctt tccagagagc ttgggttggc tggagagagg ctgtctttcc cattccttgt
     3901 ccagctagga ataaagggga aatggtccta gcctggcccc tacacaccca ggtcccacag
     3961 gccccctccc cactggaatt tcaccaacca acaaggggaa agtacgctgt tacagcatag
     4021 cggtcaggcc cagcaggagc ttggcacatg atggggaggt ggccagctcc aggccctgcc
     4081 cgaccccatc atgtgtattt ggtgtatggg gtgtgggggg tcacaccaga agctggcctg
     4141 gggtctcttc tttgctggac acagctccct ggcccctgcc cccagcccct gcagcccctg
     4201 ccggactgtg gaagccacat atgggaaaag tcctggcaga caatgtggcg ggatgactgg
     4261 gggcttctcc ctctgaacct gggtccagtg tagcctggct ctgagagaag gtggtgagca
     4321 tgtggagaag gttccatagt ccactcttag gggaaccagc aaagcctcat ggcagttggc
     4381 tccatctgga cctcccatgg tcactacagg atggtggagc agggggcatc ttttagcctc
     4441 cccccgccac cacatccagg ccctctcagg cacctcctgc ctcagcccac acctgcctca
     4501 cccattgccc ccctcccccc acctactgcc atcccactcc tctgccagcc acttcccagc
     4561 cgccccaccc cactccatcc accaaatcac ctcctgactt aatcctttct ggaaggagct
     4621 gccgcccagg aaccggtatt gcctagagcc tccaggaggg gccctcctca ggcctccagt
     4681 ggccccatgc ccacctgcct gaccctccac tgcccctgga agcaaagtgc ctatcagcag
     4741 cgttgcgtcc tctggggccc ccggtcgggg gggagggggt gtgggctaac cttggccacc
     4801 accacaaaag gaatgtgcca gaatgctgaa ccttcttgtt aatgctatga ccgtgccttg
     4861 aataaacaag tcctcccaac ctcagtcgac ctgcaggcat gcaagctgat ccggctgcta
     4921 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     4981 cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     5041 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5101 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5161 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt
     5221 ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     5281 cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg
     5341 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     5401 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5461 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5521 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5581 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5641 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5701 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag
     5761 tctcgaacca aaggctggag ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa
     5821 acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg tgctggactc
     5881 cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt ttctgcatgg
     5941 taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag
     6001 atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga atggctcata
     6061 tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga accttccaaa
     6121 gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact actcctacga
     6181 gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc
     6241 ctgggacgag tggcctgaca tcgaggagga tatcgccctg atcaagagcg aagagggcga
     6301 gaaaatggtg cttgagaata acttcttcgt cgagaccatg ctcccaagca agatcatgcg
     6361 gaaactggag cctgaggagt tcgctgccta cctggagcca ttcaaggaga agggcgaggt
     6421 tagacggcct accctctcct ggcctcgcga gatccctctc gttaagggag gcaagcccga
     6481 cgtcgtccag attgtccgca actacaacgc ctaccttcgg gccagcgacg atctgcctaa
     6541 gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg gagctaagaa
     6601 gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg aggacgctcc
     6661 agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga acgagcagac
     6721 cggtggtggg agcggaggtg gcggatcagg tggcggaggc tccggaggga ttgaacaaga
     6781 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6841 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6901 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6961 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7021 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7081 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7141 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7201 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7261 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7321 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7381 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7441 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7501 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7561 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
     7621 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     7681 agcgataagg atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     7741 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtaatgg tttcttagac
     7801 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7861 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7921 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7981 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8041 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8101 gagttttcgc cccgaagaac gttttccaat gatgagcact ttcaaagttc tgctatgtgg
     8161 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8221 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8281 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8341 tctgacaact atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8401 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8461 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8521 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8581 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8641 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8701 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8761 tgagataggt gcctcactga ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc
     8821 caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9361 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa
     9541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga
     9661 aatacaggaa cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca
     9721 gcttcagtgg attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat
     9781 acggttaaaa tttatcaggc gcgattgcgg cagtttttcg ggtggtttgt tgccattttt
     9841 acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt
     9901 atggtaaatc cacttactgt ctgccctcgt agccatcgag ataaaccgca gtactccggc
     9961 cacgatgcgt ccggcgtaga ggatcgagat ctaccgggta ggggaggcgc ttttcccaag
    10021 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
    10081 ctggcctcgc acacattcca cat
//
