LOCUS       sequence-231683-        8982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 8982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-SH3GLB2-203
FEATURES             Location/Qualifiers
     source          1..8982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             71..1723
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3523..3644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3534..3553)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3588..3607
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3889..4246
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3889..4080
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3912..3932)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4097..4232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      4291..4300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4297..5229
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             5281..6072
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5332..5351)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5942..5961
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6136..6184
                     /note="synthetic polyadenylation signal"
     promoter        6340..6444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6445..6513,6514..7305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6663..6682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7463..8051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7543..7562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8167..8450
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(8507..8982,1..24)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8542..8565)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8939..8958
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tcttcctcat ctccgggcct ttcgacctgc agcccaagct tggcaatccg gtactgttgg
       61 taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
      121 cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc
      181 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
      241 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
      301 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
      361 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
      421 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
      481 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
      541 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
      601 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
      661 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
      721 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
      781 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
      841 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
      901 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa
      961 gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     1021 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     1081 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     1141 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     1201 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     1261 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     1321 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     1381 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     1441 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     1501 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     1561 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     1621 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     1681 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taattctagt tgtttaaacg
     1741 agctcggcag gtgcccccat cccccccgca ttctggccta ggcaggagag gatgggcgca
     1801 gccctgccac ttaacttgtt tgttggtgac acagttgttc agagtgggga gaattcaccc
     1861 cattctgtcc ctgcccctag tcacctagct gtgagggtgc ctgaggctga atggctccac
     1921 ccctccccca gccctgcttc tgacctgtgg ctctggagcc cctgcccctg cctgcatccc
     1981 cgagcacccc accctccagg ctccactaag gagggagggg ctgtctgcag cagctgcact
     2041 cagcacctag gccagggtgg ggccgccgca gatgggctca ggaagcccca ggtgcactca
     2101 gcgagagccc tgcctttcag ttgccaaaag ctgcatcagg ggaatgcggc aaggcacaca
     2161 gggctctggc agcccctggg gactgggcgc tgcccctggg aggggagagc ctggccaggg
     2221 ctggtgttgg gcccggagca gcatcttccg gtgctatcct cccctcccac ccctcacagc
     2281 tcaagccaag tccagcggcc gcagtcttca cctctccaca ctcacttttt atctggtgtt
     2341 tttacttctg cctgcgtttg ctctctagcc aataaaccgt ccttgtgtgc gagtcacctg
     2401 ggctcctgtc agggcctggc cctgagggtg gtaaagggaa aggtagactc accctctgcc
     2461 ccactcttcc acagagtgag caggggccct gtggctgccc cggcccccag ctctctagtt
     2521 gggaagaagt gcagccctcc ctgtcttctg cggggcctgt gcctctcaga gggcagtgct
     2581 gttcctggac atgaagccgc acaaggaacg ttttattcct aagaaaaagt ttcctaaatt
     2641 tggattgtgg agcggactgt agaatgcgtc tgacagttca gttcaagcac aagaagtggg
     2701 tagcggtcag tgctgtctgc aggtcgggcg tttcacccag catgtgcccc cgtgccatgg
     2761 gttctcatgc cgtcctcagt gtccacattt cacagatgag atcagcaaga cagtcagctc
     2821 ctcaagggac cacagttggc aagtggccaa gcagggactg aagcccaggc agcctgattc
     2881 tagaaaccct aagtgatgtc tgtgttttag atggccccta gcaggaccta gcattctgac
     2941 ttcagtcctg agaaggccaa cagccagtag gccccactgt tggggcttct gaagagggga
     3001 tcgggggatg acgggggcac ggggctataa ataggaaggc acagagcttc aagaaaggct
     3061 cacccaggga tgagctcagc ccactgggcc aatgaaagca gggtcaagga caggaccagc
     3121 gcaggccaag gaagggaata tctgacagcg cccacccagc caaaccctca gcccaaggac
     3181 aggaatgagg agatgctggt gaactagcca tccatcagta cctgccttcc cccgaggctg
     3241 cagccccact cccaggcgcc tggccagggg agttttctag gttctgagag ccacgttgtc
     3301 atccctgggc tttgaagtta aacatcacac agctgtctat aaacaagatt ttaatatctg
     3361 tataaataga ccacagaatt accaaaactg caaaagtcga cctgcaggca tgcaagctga
     3421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     3481 actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt
     3541 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3601 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3661 ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc
     3721 tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     3781 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc
     3841 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
     3901 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4021 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4081 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4201 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgattcttc
     4261 tgacacaaca gtctcgaacc aaaggctgga gccaccatgg cttccaaggt gtacgacccc
     4321 gagcaacgca aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac
     4381 gtgctggact ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt
     4441 tttctgcatg gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag
     4501 cccgtggcta gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg
     4561 aatggctcat atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg
     4621 aaccttccaa agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac
     4681 tactcctacg agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac
     4741 gtgatcgagt cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc
     4801 gaagagggcg agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc
     4861 aagatcatgc ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag
     4921 aagggcgagg ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga
     4981 ggcaagcccg acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac
     5041 gatctgccta agatgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag
     5101 ggagctaaga agttccctaa caccgagttc gtgaaggtga agggcctcca cttcagccag
     5161 gaggacgctc cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag
     5221 aacgagcaga ccggtggtgg gagcggaggt ggcggatcag gtggcggagg ctccggaggg
     5281 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5341 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5401 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5461 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5521 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5581 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5641 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5701 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5761 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5821 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5881 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5941 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6001 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6061 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     6121 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     6181 tgtgaatcga tagcgataag gatcctcttt gcgcttgcgt tttcccttgt ccagatagcc
     6241 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtaatg
     6301 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6361 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6421 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6481 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6541 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6601 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac tttcaaagtt
     6661 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6721 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6781 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6841 gccaacttac ttctgacaac tatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6901 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6961 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7021 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7081 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7141 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7201 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7261 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaattcga aatgaccgac
     7321 caagcgacgc ccaaccggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7861 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8041 aaggatttca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8161 tatagtccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
     8221 gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca
     8281 taggtattca tacggttaaa atttatcagg cgcgattgcg gcagtttttc gggtggtttg
     8341 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca
     8401 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
     8461 agtactccgg ccacgatgcg tccggcgtag aggatcgaga tctaccgggt aggggaggcg
     8521 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     8581 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     8641 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     8701 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     8761 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     8821 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     8881 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     8941 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cc
//
