LOCUS       sequence-231685-        9510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-FAM104A-202
FEATURES             Location/Qualifiers
     source          1..9510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     375..394
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      483..492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..2141
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4469..4590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4480..4499)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4534..4553
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4835..5192
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4835..5026
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4858..4878)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5043..5178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5237..5246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5243..6175
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6227..7018
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6278..6297)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6888..6907
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7082..7130
                     /note="synthetic polyadenylation signal"
     promoter        7286..7390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7391..7459,7460..8251)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7609..7628)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8409..8997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8489..8508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9113..9396
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(9453..9510,1..442)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(join(9488..9510,1))
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
       61 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      121 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      181 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
      241 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
      301 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
      361 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
      421 ttcctcatct ccgggccttt cgacctgcag cccaagcttg gcaatccggt actgttggta
      481 aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg
      541 aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg
      601 gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg
      661 agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga
      721 tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca
      781 tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca
      841 tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca
      901 acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact
      961 accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg
     1021 agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca
     1081 gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc
     1141 gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc
     1201 tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct
     1261 gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc
     1321 aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga
     1381 gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc
     1441 cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc
     1501 agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca
     1561 agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca
     1621 ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca
     1681 tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc
     1741 tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc
     1801 tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc
     1861 tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg
     1921 gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga
     1981 tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt
     2041 tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga
     2101 ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta attctagttg tttaaacgag
     2161 ctcgtgtgcc ggcagtttct tgccttctgt gaagggacag cgctgtgcag atttgatatt
     2221 tcaacttaca acttgtttta aaagaaaaat tgcacacgaa aaatgcctgt tggcttttca
     2281 gtctatattt gaaataacag gttaacaggc agttgtttac ttgtggtttt gctgcactat
     2341 tgcaatttca aaggggcttt gaagcaattt tttataggat tcttttgagg gagggtatca
     2401 aatctatgtc aaggtagtgg tttgaggaaa actcattctg tgtgttgaat ccatagtatg
     2461 tcccattcag actgatttcc aaatctgtca actagaaact ctactttatg taaagggcct
     2521 tttaaaaatg cgggatcttc aatttttttt attcaataaa ctagtaaaga gtatctagtt
     2581 tccatgaaaa aacataagtt tttttccaaa atataggaag cttgattcag tcttgcactg
     2641 aaattaactg ccatactacc tctcagtatg tagatgttct attgaaagta ctctctcctg
     2701 tgtaacagga gaacaatcag atccaattca gactgtcagt gaagcacact ccctaataat
     2761 agtctctact ctttgttata tatttgtatt tccagggaga atgtctttta gattcttgta
     2821 tacccagata acttctgtgt cactattcca ccagagtctg cacctcagac tttagtagga
     2881 aacatggcac agttctgcaa gtaaagtaat atgtaatgac tttttgtcct acaaacttgg
     2941 tcagtcagca gcttacatga tctcattgac aattccacct tgtagtgtca acaattaagg
     3001 tgttgttgaa cgctgataaa atgtaagcta cctgaactga tgtctaaagc aacaagtatt
     3061 gtgaaatttt gggacaagga ttaacttgat gcttaaagta acccccaaca gtaactaggg
     3121 ggccacattc catcagtgaa actggaaaga ttggtgtggc tgagaatcag atgagcagag
     3181 gcacctttag aagaaataat gtggtagtat ttatttaggg agagacagat gtgttgccat
     3241 gttacttagt catactagag agccagccaa gtttgaataa tgaaggtgca gacgtgttgt
     3301 cttcttcact gacaagctgt ctgaacaagg agccaggaac tgtcgggtca ccagctgtgg
     3361 agcgtgccgg ggggacaacc tgccattgga tgtagagaaa agtgccttag agttggcctg
     3421 agccctcctg cccactcgcc ctccgcgcga cgccactcgg agtccacacg gcacacacaa
     3481 gcggagctga gaagcacgat ggctctagtc agaggacttt gctgtcggtc cagatttgaa
     3541 gaggacacgg aacagcatga gcctttgtga caaaactaga atttggaaat gaggactgtc
     3601 ttgggaaaag ggtagacaca ggcacttctc tccacagcct gctaacctaa tgtgggcaga
     3661 actgaaagat cactgcagtg gaaactgttc tggaagaggc tgcaacatag cccatgctta
     3721 gggactggat ggtttttgca agagcttttt ttttttttaa actaccatct cccaactttt
     3781 aaagccagat acttgacaag gattttagat cttaatgggt tcgatggtag ggaatacgga
     3841 aaggatacac tgctaatatt gtgtctaaca ataaagcaaa caactcgatg ctgtaagaag
     3901 acaagctgtt tgagctttca aacacacatg cacacagcac aattcagaaa tacccaggag
     3961 gattcctgca ggcttaacag ttggcatcgt actgaaatga aacgtgtatt aacaagaaaa
     4021 tgtcaaactt ttggggaaac aaattttctg ttacttccca cccaactaaa agggaagata
     4081 aaaccatcat caccagaaca aaacagattt tctccaaaca agtacaatga tagtgccggg
     4141 ttggcagtta actctttcac cctactaaat tcaagagctc atctccaccc tgtcctgtat
     4201 attttctaca tttctattaa gaggctactt ggtagtgtca gcgggcatct tttacacctt
     4261 ctagtagctc aagctagtaa aaccttgtac ttctctattg cgccgccatt aaaagcagca
     4321 ttagtgcatt ttgtcattca agtcgacctg caggcatgca agctgatccg gctgctaaca
     4381 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     4441 ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     4501 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4561 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4621 caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt
     4681 aaaatcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     4741 acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa
     4801 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
     4861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5161 gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct
     5221 cgaaccaaag gctggagcca ccatggcttc caaggtgtac gaccccgagc aacgcaaacg
     5281 catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt
     5341 catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa
     5401 cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg
     5461 catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg
     5521 cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa
     5581 aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca
     5641 ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg
     5701 ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa
     5761 aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa
     5821 actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag
     5881 acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt
     5941 cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat
     6001 gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt
     6061 ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga
     6121 tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagaccgg
     6181 tggtgggagc ggaggtggcg gatcaggtgg cggaggctcc ggagggattg aacaagatgg
     6241 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6301 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6361 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6421 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6481 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6541 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6601 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6661 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6721 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6781 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6841 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6901 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6961 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7021 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg
     7081 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc
     7141 gataaggatc ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
     7201 catccggggt cagcaccgtt tctgcggact ggctttctac gtaatggttt cttagacgtc
     7261 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7321 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7381 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7441 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7501 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7561 ttttcgcccc gaagaacgtt ttccaatgat gagcactttc aaagttctgc tatgtggcgc
     7621 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7681 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7741 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7801 gacaactatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7861 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7921 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7981 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8041 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8101 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8161 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8221 gataggtgcc tcactgatta agcattggta attcgaaatg accgaccaag cgacgcccaa
     8281 ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8821 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atttcaagaa
     9001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata gtccggaaat
     9121 acaggaacgc acgctggatg gcccttcgct gggatggtga aaccatgaaa aatggcagct
     9181 tcagtggatt aagtgggggt aatgtggcct gtaccctctg gttgcatagg tattcatacg
     9241 gttaaaattt atcaggcgcg attgcggcag tttttcgggt ggtttgttgc catttttacc
     9301 tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg cgtacaatta agggattatg
     9361 gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagta ctccggccac
     9421 gatgcgtccg gcgtagagga tcgagatcta ccgggtaggg gaggcgcttt tcccaaggca
     9481 gtctggagca tgcgctttag cagccccgct
//
