LOCUS       sequence-231690-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes full-length human Tie1 gene fused in-frame with 
            Cherry cloned in lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-TIE1-Cherry
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1241..1250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1784..1795)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4706..5413
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4848..4866)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5116..5135
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5446..6034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5499..5519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5917..5928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6037..6053)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6038..6054)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6559..6739
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6765..6782)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6771..6995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7041..7469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7128..7147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7338..7359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7478..7498)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7483..7812
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7663..7798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7725..7744
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7860..7907
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7926..8300
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8430..8551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8467..8486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8521..8540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8600..8616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8600..8616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8613..8635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8624..8640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8648..8654,8655..8672,8673..8678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8693..8714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8831..8848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9002..9590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9082..9101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9761..10552,10553..10621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10384..10403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10622..10726)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10801..10820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10992..11371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11373..11571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11526..11546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11589..11769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11816..11941
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12434..12667
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12852..12896
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13194..13311
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatgg tctggcgggt
     1261 gccccctttc ttgctcccca tcctcttctt ggcttctcat gtgggcgcgg cggtggacct
     1321 gacgctgctg gccaacctgc ggctcacgga cccccagcgc ttcttcctga cttgcgtgtc
     1381 tggggaggcc ggggcgggga ggggctcgga cgcctggggc ccgcccctgc tgctggagaa
     1441 ggacgaccgt atcgtgcgca ccccgcccgg gccacccctg cgcctggcgc gcaacggttc
     1501 gcaccaggtc acgcttcgcg gcttctccaa gccctcggac ctcgtgggcg tcttctcctg
     1561 cgtgggcggt gctggggcgc ggcgcacgcg cgtcatctac gtgcacaaca gccctggagc
     1621 ccacctgctt ccagacaagg tcacacacac tgtgaacaaa ggtgacaccg ctgtactttc
     1681 tgcacgtgtg cacaaggaga agcagacaga cgtgatctgg aagagcaacg gatcctactt
     1741 ctacaccctg gactggcatg aagcccagga tgggcggttc ctgctgcagc tcccaaatgt
     1801 gcagccacca tcgagcggca tctacagtgc cacttacctg gaagccagcc ccctgggcag
     1861 cgccttcttt cggctcatcg tgcggggttg tggggctggg cgctgggggc caggctgtac
     1921 caaggagtgc ccaggttgcc tacatggagg tgtctgccac gaccatgacg gcgaatgtgt
     1981 atgcccccct ggcttcactg gcacccgctg tgaacaggcc tgcagagagg gccgttttgg
     2041 gcagagctgc caggagcagt gcccaggcat atcaggctgc cggggcctca ccttctgcct
     2101 cccagacccc tatggctgct cttgtggatc tggctggaga ggaagccagt gccaagaagc
     2161 ttgtgcccct ggtcattttg gggctgattg ccgactccag tgccagtgtc agaatggtgg
     2221 cacttgtgac cggttcagtg gttgtgtctg cccctctggg tggcatggag tgcactgtga
     2281 gaagtcagac cggatccccc agatcctcaa catggcctca gaactggagt tcaacttaga
     2341 gacgatgccc cggatcaact gtgcagctgc agggaacccc ttccccgtgc ggggcagcat
     2401 agagctacgc aagccagacg gcactgtgct cctgtccacc aaggccattg tggagccaga
     2461 gaagaccaca gctgagttcg aggtgccccg cttggttctt gcggacagtg ggttctggga
     2521 gtgccgtgtg tccacatctg gcggccaaga cagccggcgc ttcaaggtca atgtgaaagt
     2581 gccccccgtg cccctggctg cacctcggct cctgaccaag cagagccgcc agcttgtggt
     2641 ctccccgctg gtctcgttct ctggggatgg acccatctcc actgtccgcc tgcactaccg
     2701 gccccaggac agtaccatgg actggtcgac cattgtggtg gaccccagtg agaacgtgac
     2761 gttaatgaac ctgaggccaa agacaggata cagtgttcgt gtgcagctga gccggccagg
     2821 ggaaggagga gagggggcct gggggcctcc caccctcatg accacagact gtcctgagcc
     2881 tttgttgcag ccgtggttgg agggctggca tgtggaaggc actgaccggc tgcgagtgag
     2941 ctggtccttg cccttggtgc ccgggccact ggtgggcgac ggtttcctgc tgcgcctgtg
     3001 ggacgggaca cgggggcagg agcggcggga gaacgtctca tccccccagg cccgcactgc
     3061 cctcctgacg ggactcacgc ctggcaccca ctaccagctg gatgtgcagc tctaccactg
     3121 caccctcctg ggcccggcct cgccccctgc acacgtgctt ctgcccccca gtgggcctcc
     3181 agccccccga cacctccacg cccaggccct ctcagactcc gagatccagc tgacatggaa
     3241 gcacccggag gctctgcctg ggccaatatc caagtacgtt gtggaggtgc aggtggctgg
     3301 gggtgcagga gacccactgt ggatagacgt ggacaggcct gaggagacaa gcaccatcat
     3361 ccgtggcctc aacgccagca cgcgctacct cttccgcatg cgggccagca ttcaggggct
     3421 cggggactgg agcaacacag tagaagagtc caccctgggc aacgggctgc aggctgaggg
     3481 cccagtccaa gagagccggg cagctgaaga gggcctggat cagcagctga tcctggcggt
     3541 ggtgggctcc gtgtctgcca cctgcctcac catcctggcc gcccttttaa ccctggtgtg
     3601 catccgcaga agctgcctgc atcggagacg caccttcacc taccagtcag gctcgggcga
     3661 ggagaccatc ctgcagttca gctcagggac cttgacactt acccggcggc caaaactgca
     3721 gcccgagccc ctgagctacc cagtgctaga gtgggaggac atcacctttg aggacctcat
     3781 cggggagggg aacttcggcc aggtcatccg ggccatgatc aagaaggacg ggctgaagat
     3841 gaacgcagcc atcaaaatgc tgaaagagta tgcctctgaa aatgaccatc gtgactttgc
     3901 gggagaactg gaagttctgt gcaaattggg gcatcacccc aacatcatca acctcctggg
     3961 ggcctgtaag aaccgaggtt acttgtatat cgctattgaa tatgccccct acgggaacct
     4021 gctagatttt ctgcggaaaa gccgggtcct agagactgac ccagcttttg ctcgagagca
     4081 tgggacagcc tctaccctta gctcccggca gctgctgcgt ttcgccagtg atgcggccaa
     4141 tggcatgcag tacctgagtg agaagcagtt catccacagg gacctggctg cccggaacgt
     4201 gctggtcgga gagaacctag cctccaagat tgcagacttc ggcctttctc ggggagagga
     4261 ggtttatgtg aagaagacga tggggcgtct ccctgtgcgc tggatggcca ttgagtccct
     4321 gaactacagt gtctatacca ccaagagtga tgtctggtcc tttggagtcc ttctttggga
     4381 gatagtgagc cttggaggta caccctactg tggcatgacc tgtgccgagc tctatgaaaa
     4441 gctgccccag ggctaccgca tggagcagcc tcgaaactgt gacgatgaag tgtacgagct
     4501 gatgcgtcag tgctggcggg accgtcccta tgagcgaccc ccctttgccc agattgcgct
     4561 acagctaggc cgcatgctgg aagccaggaa ggcctatgtg aacatgtcgc tgtttgagaa
     4621 cttcacttac gcgggcattg atgccacagc tgaggaggcc ggagggggag gttctggcgg
     4681 aggaggatcg ggtggtggtg gttcagtgag caagggcgag gaggataaca tggccatcat
     4741 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     4801 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     4861 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     4921 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     4981 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5041 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5101 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5161 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5221 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     5281 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     5341 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     5401 gctgtacaag taaggcgcgc cgaattcgat atcaagctta tcgataatca acctctggat
     5461 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5521 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5581 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5641 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5701 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5761 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5821 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5881 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5941 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6001 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     6061 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6121 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6181 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6241 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     6301 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     6361 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     6421 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     6481 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     6541 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6721 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     6781 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6841 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6901 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6961 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7021 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7381 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7441 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7501 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7561 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7621 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7681 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7741 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7801 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     7861 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     7921 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     7981 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     8041 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     8101 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     8161 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     8221 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     8281 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     8341 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8401 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8581 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8641 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8701 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8761 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8821 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8881 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8941 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9001 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9061 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9421 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10741 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    10801 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    10861 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    10921 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
    10981 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    11041 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    11101 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    11161 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    11221 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    11281 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11341 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
    11401 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    11461 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
    11521 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
    11581 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    11641 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    11701 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    11761 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
    11821 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    11881 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    11941 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    12001 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    12061 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    12121 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    12181 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    12241 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    12301 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    12361 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    12421 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    12481 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    12541 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    12601 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    12661 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    12721 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    12781 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    12841 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    12901 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    12961 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    13021 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    13081 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    13141 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
    13201 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    13261 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    13321 tacagggaca gcagagatcc agtttggtta attaacccgt gtcggctcca gatct
//
