LOCUS       sequence-231699-        9359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty vector for cloning shRNA and/or miRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9359)
  TITLE     Improved vector system for knockdown
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-H1-SGIPZ
FEATURES             Location/Qualifiers
     source          1..9359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             61..694
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    741..866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1363..1596
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1781..1825
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2123..2240
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2305..2679)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2676..2685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2689..2707)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2699..2746)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2774..2864
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2785..2804
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     enhancer        2963..3266
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3267..3470
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3421..3441
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3467..3491
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3556..3565
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3562..4260
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    4287..4859
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4454..4471)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4681..4700
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4862..5464
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5361..5381
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5689..6277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5742..5762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6160..6171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6484..6717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6818..6835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6824..6935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7146..7734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7226..7245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7905..8696,8697..8765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8528..8547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8766..8870)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8942..9077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9118..9252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9155..9174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9209..9228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
       61 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
      121 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      181 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      241 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      301 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      361 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      421 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      481 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1261 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1321 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1381 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1441 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1501 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1561 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1621 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1681 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1741 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1801 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1861 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1921 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1981 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2041 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2101 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2161 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2221 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2281 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2341 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2401 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2461 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2521 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2581 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2641 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2701 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2761 cgaggttcta gtaatatttg catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa
     2821 atgtctttgg atttgggaat cttataagtt ctgtatgaga ccacatcgat tctttttttc
     2881 tagacgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
     2941 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3001 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3061 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3121 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3181 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3241 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3301 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3361 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3421 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     3481 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
     3541 tctactagag gatctgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     3601 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     3661 ggcacccccg agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     3721 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     3781 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     3841 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     3901 gccggccgcg tgatcggcga cttcaaggtg atgggcaccg gcttccccga ggacagcgtg
     3961 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     4021 gataacgatc tggatggcag cttcacccgc accttcagcc tgcgcgacgg cggctactac
     4081 agctccgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     4141 aacgggggcc ccatgttcgc cttccgccgc gtggaggagg atcacagcaa caccgagctg
     4201 ggcatcgtgg agtaccagca cgccttcaag accccggatg cagatgccgg tgaagaataa
     4261 tgtacaagta gcggccgcaa attccgcccc tctccctccc ccccccctaa cgttactggc
     4321 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     4381 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     4441 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     4501 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     4561 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     4621 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     4681 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4741 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4801 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
     4861 catggccacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     4921 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     4981 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5041 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5101 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag
     5161 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5221 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5281 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt
     5341 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5401 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5461 ctgagtttgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa
     5521 acacttgctg ggattacttc ttcaggttaa cccaacagaa ttctcttctg aagaaaatat
     5581 atttcttttt attcatagct cttctcgagg taaccggatc cagttgtgtt ttaatgtata
     5641 ttaatgttac taatgtgttt tatttaaatt gagtcacgcg tctggaacaa tcaacctctg
     5701 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5761 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5821 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5881 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5941 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6001 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6061 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6121 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6181 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6241 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     6301 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6361 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6421 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     6481 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6721 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6781 gtgagaggcc ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     6841 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6901 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6961 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8881 cgaaaagtgc cacctgacgt cgagttagtc agcaaccata gtcccgcccc taactccgcc
     8941 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9001 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9061 aggctttttt ggaggcctag gcttttgcaa aaagcttcgt cgacggatcg ggagatcaac
     9121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9241 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     9301 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctc
//
