LOCUS       sequence-23170-e         415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, 
            Kinzler KW, Vogelstein B
  TITLE     Mutations of mitotic checkpoint genes in human cancers.
  JOURNAL   Nature. 1998 Mar 19. 392(6673):300-3.
  PUBMED    9521327
REFERENCE   2  (bases 1 to 415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1998 Mar 19. 392(6673):300-3."
COMMENT     Sequence Label: pBI-GFP-Bub1-deletion mutant
FEATURES             Location/Qualifiers
     source          1..415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      259..268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(358..382)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgccggccgc gtttcagtga ggcgtgtctg aaataacaaa ttttaaacaa taactgatga
       61 atcttgggtc attgtggtat ttcttcttat ctaaaaattc cttcattaaa tgttctagta
      121 aagttatcaa gtattcttta ttctcaggaa aattctcttc tacccactgt atgtatcttt
      181 cccattcacc aagagggtca ttgcccttgt agctctgcat gtgggcttca agcatctgaa
      241 ggacattttc cggggtgtcc atggtggcgg ccgctgcaag aattcggggc cgcggaagct
      301 ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat
      361 ctgacggttc actaaacgag ctctgcttat ataggtcgag tttaccactc cctat
//
