LOCUS       sequence-231714-        6000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Plevy SE, Gemberling JH, Hsu S, Dorner AJ, Smale ST
  TITLE     Multiple control elements mediate activation of the murine and human
            interleukin 12 p40 promoters: evidence of functional synergy between
            C/EBP and Rel proteins.
  JOURNAL   Mol Cell Biol. 1997 Aug . 17(8):4572-88.
  PUBMED    9234715
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1997 Aug . 17(8):4572-88."
COMMENT     Sequence Label: Il12b 400bp pro-Luc
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    646..767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1270..2922
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1336..1354)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3163..3228
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3358..3378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3803..3937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4079..4096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4250..4838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4330..4349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5009..5800,5801..5869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5632..5651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5870..5974)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgtggag gttttacttg ctttaaaaaa cctcccacac
      601 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatgg tactgtaact
      781 gagctaacat aacccgggag gtaccgagct cttacgcgtt tggggcaagt ctttcctttt
      841 tctgcagtct gtggaatcgg gagaggttag ccattgccgc ctctattcac cttaggcatg
      901 atgtaaacag aaattagtat ctctgcctcc ttcctttttc cacaccccga agtcatttcc
      961 tcttaacctg ggatttcgac gtctatattc cctctgtatg atagatgcac tcagggaggc
     1021 aaggggggga gggaggaact tcttaaaatt cccccagaat gttttgacac tagttttcag
     1081 tgttgcaatt gagactagtc agtttctact ttgggtttcc atcagaaagt tctgtaggag
     1141 tagagtatat aagcaccagg agcagccaag gcagcagaag gaacagtggg tgtccaggca
     1201 catcagacca ggcagctcgc agcaaagcaa agatctaagt aagcttggca ttccggtact
     1261 gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta
     1321 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     1381 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
     1441 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     1501 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     1561 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     1621 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
     1681 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
     1741 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     1801 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat
     1861 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
     1921 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     1981 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     2041 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
     2101 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
     2161 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
     2221 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
     2281 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     2341 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     2401 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
     2461 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     2521 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
     2581 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata
     2641 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
     2701 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     2761 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     2821 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     2881 atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac tgtattcagc
     2941 gatgacgaaa ttcttagcta ttgtaatact gcgatgagtg gcagggcggg gcgtaatttt
     3001 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     3061 agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt ctgtggtgtg
     3121 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3181 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3241 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     3301 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     3361 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     3421 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     3481 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     3541 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     3601 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     3661 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     3721 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3781 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3901 gtccaaactc atcaatgtat cttatcatgt ctggatccgt cgaccgatgc ccttgagagc
     3961 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     4021 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     4081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
