LOCUS       sequence-231727-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-132-3p
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(95..119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(343..362)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    408..463
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(462..481)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aaagagacat acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
       61 aaactggggc acaaacttaa ttgagaattc taccacccag ctttcttgta caaagtggta
      121 cgtctcaaga ccagaggtct ctgcttatag cgatcgacca tggctgtaga ctgttacgac
      181 catggctgta gactgttacg accatggctg tagactgtta cgaccatggc tgtagactgt
      241 tacaaccaga agagacctgt gcttcactcc tcaggtgcag gctgcctatc agaaggtggt
      301 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
      361 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      421 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
      481 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
      541 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
      601 gccccctgct gtccattcct tattccatag aaaagccttg ac
//
