LOCUS       sequence-231731-       14059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes full-length human VEGFR-3 gene fused in-frame with 
            EGFP cloned in lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14059)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 14059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-hR3-EGFP
FEATURES             Location/Qualifiers
     source          1..14059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(4169..4171,4172..4885)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4169..4171,4172..4882)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4211..4232)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4472..4491)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4819..4840
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4918..5506
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4971..4991)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5389..5400)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5509..5525)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5510..5526)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6031..6211
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6237..6254)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6243..6467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6513..6941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6600..6619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6810..6831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6950..6970)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6955..7284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7135..7270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7197..7216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7332..7379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7398..7772
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7902..8023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7939..7958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7993..8012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8072..8088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8072..8088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8085..8107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8096..8112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8120..8126,8127..8144,8145..8150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8165..8186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8303..8320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8474..9062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8554..8573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9233..10024,10025..10093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9856..9875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10094..10198)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10273..10292)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10464..10843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10845..11043
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10998..11018
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11061..11241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11288..11413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11906..12139
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12324..12368
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12666..12783
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        12848..14059
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     13896..13915
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gaagcttggg ctgcaggtcg actctagagc caccatgcag cggggcgccg cgctgtgcct
       61 gcgactgtgg ctctgcctgg gactcctgga cggcctggtg agtggctact ccatgacccc
      121 cccgaccttg aacatcacgg aggagtcaca cgtcatcgac accggtgaca gcctgtccat
      181 ctcctgcagg ggacagcacc ccctcgagtg ggcttggcca ggagctcagg aggcgccagc
      241 caccggagac aaggacagcg aggacacggg ggtggtgcga gactgcgagg gcacagacgc
      301 caggccctac tgcaaggtgt tgctgctgca cgaggtacat gccaacgaca caggcagcta
      361 cgtctgctac tacaagtaca tcaaggcacg catcgagggc accacggccg ccagctccta
      421 cgtgttcgtg agagactttg agcagccatt catcaacaag cctgacacgc tcttggtcaa
      481 caggaaggac gccatgtggg tgccctgtct ggtgtccatc cccggcctca atgtcacgct
      541 gcgctcgcaa agctcggtgc tgtggccaga cgggcaggag gtggtgtggg atgaccggcg
      601 gggcatgctc gtgtccacgc cactgctgca cgatgccctg tacctgcagt gcgagaccac
      661 ctggggagac caggacttcc tttccaaccc cttcctggtg cacatcacag gcaacgagct
      721 ctatgacatc cagctgttgc ccaggaagtc gctggagctg ctggtagggg agaagctggt
      781 cctgaactgc accgtgtggg ctgagtttaa ctcaggtgtc acctttgact gggactaccc
      841 agggaagcag gcagagcggg gtaagtgggt gcccgagcga cgctcccagc agacccacac
      901 agaactctcc agcatcctga ccatccacaa cgtcagccag cacgacctgg gctcgtatgt
      961 gtgcaaggcc aacaacggca tccagcgatt tcgggagagc accgaggtca ttgtgcatga
     1021 aaatcccttc atcagcgtcg agtggctcaa aggacccatc ctggaggcca cggcaggaga
     1081 cgagctggtg aagctgcccg tgaagctggc agcgtacccc ccgcccgagt tccagtggta
     1141 caaggatgga aaggcactgt ccgggcgcca cagtccacat gccctggtgc tcaaggaggt
     1201 gacagaggcc agcacaggca cctacaccct cgccctgtgg aactccgctg ctggcctgag
     1261 gcgcaacatc agcctggagc tggtggtgaa tgtgcccccc cagatacatg agaaggaggc
     1321 ctcctccccc agcatctact cgcgtcacag ccgccaggcc ctcacctgca cggcctacgg
     1381 ggtgcccctg cctctcagca tccagtggca ctggcggccc tggacaccct gcaagatgtt
     1441 tgcccagcgt agtctccggc ggcggcagca gcaagacctc atgccacagt gccgtgactg
     1501 gagggcggtg accacgcagg atgccgtgaa ccccatcgag agcctggaca cctggaccga
     1561 gtttgtggag ggaaagaata agactgtgag caagctggtg atccagaatg ccaacgtgtc
     1621 tgccatgtac aagtgtgtgg tctccaacaa ggtgggccag gatgagcggc tcatctactt
     1681 ctatgtgacc accatccccg acggcttcac catcgaatcc aagccatccg aggagctact
     1741 agagggccag ccggtgctcc tgagctgcca agccgacagc tacaagtacg agcatctgcg
     1801 ctggtaccgc ctcaacctgt ccacgctgca cgatgcgcac gggaacccgc ttctgctcga
     1861 ctgcaagaac gtgcatctgt tcgccacccc tctggccgcc agcctggagg aggtggcacc
     1921 tggggcgcgc cacgccacgc tcagcctgag tatcccccgc gtcgcgcccg agcacgaggg
     1981 ccactatgtg tgcgaagtgc aagaccggcg cagccatgac aagcactgcc acaagaagta
     2041 cctgtcggtg caggccctgg aagcccctcg gctcacgcag aacttgaccg acctcctggt
     2101 gaacgtgagc gactcgctgg agatgcagtg cttggtggcc ggagcgcacg cgcccagcat
     2161 cgtgtggtac aaagacgaga ggctgctgga ggaaaagtct ggagtcgact tggcggactc
     2221 caaccagaag ctgagcatcc agcgcgtgcg cgaggaggat gcgggacgct atctgtgcag
     2281 cgtgtgcaac gccaagggct gcgtcaactc ctccgccagc gtggccgtgg aaggctccga
     2341 ggataagggc agcatggaga tcgtgatcct tgtcggtacc ggcgtcatcg ctgtcttctt
     2401 ctgggtcctc ctcctcctca tcttctgtaa catgaggagg ccggcccacg cagacatcaa
     2461 gacgggctac ctgtccatca tcatggaccc cggggaggtg cctctggagg agcaatgcga
     2521 atacctgtcc tacgatgcca gccagtggga attcccccga gagcggctgc acctggggag
     2581 agtgctcggc tacggcgcct tcgggaaggt ggtggaagcc tccgctttcg gcatccacaa
     2641 gggcagcagc tgtgacaccg tggccgtgaa aatgctgaaa gagggcgcca cggccagcga
     2701 gcaccgcgcg ctgatgtcgg agctcaagat cctcattcac atcggcaacc acctcaacgt
     2761 ggtcaacctc ctcggggcgt gcaccaagcc gcagggcccc ctcatggtga tcgtggagtt
     2821 ctgcaagtac ggcaacctct ccaacttcct gcgcgccaag cgggacgcct tcagcccctg
     2881 cgcggagaag tctcccgagc agcgcggacg cttccgcgcc atggtggagc tcgccaggct
     2941 ggatcggagg cggccgggga gcagcgacag ggtcctcttc gcgcggttct cgaagaccga
     3001 gggcggagcg aggcgggctt ctccagacca agaagctgag gacctgtggc tgagcccgct
     3061 gaccatggaa gatcttgtct gctacagctt ccaggtggcc agagggatgg agttcctggc
     3121 ttcccgaaag tgcatccaca gagacctggc tgctcggaac attctgctgt cggaaagcga
     3181 cgtggtgaag atctgtgact ttggccttgc ccgggacatc tacaaagacc ctgactacgt
     3241 ccgcaagggc agtgcccggc tgcccctgaa gtggatggcc cctgaaagca tcttcgacaa
     3301 ggtgtacacc acgcagagtg acgtgtggtc ctttggggtg cttctctggg agatcttctc
     3361 tctgggggcc tccccgtacc ctggggtgca gatcaatgag gagttctgcc agcggctgag
     3421 agacggcaca aggatgaggg ccccggagct ggccactccc gccatacgcc gcatcatgct
     3481 gaactgctgg tccggagacc ccaaggcgag acctgcattc tcggagctgg tggagatcct
     3541 gggggacctg ctccagggca ggggcctgca agaggaagag gaggtctgca tggccccgcg
     3601 cagctctcag agctcagaag agggcagctt ctcgcaggtg tccaccatgg ccctacacat
     3661 cgcccaggct gacgctgagg acagcccgcc aagcctgcag cgccacagcc tggccgccag
     3721 gtattacaac tgggtgtcct ttcccgggtg cctggccaga ggggctgaga cccgtggttc
     3781 ctccaggatg aagacatttg aggaattccc catgacccca acgacctaca aaggctctgt
     3841 ggacaaccag acagacagtg ggatggtgct ggcctcggag gagtttgagc agatagagag
     3901 caggcataga caagaaagcg gcttcagctg taaaggacct ggccagaatg tggctgtgac
     3961 cagggcacac cctgactccc aagggaggcg gcggcggcct gagcgggggg cccgaggagg
     4021 ccaggtgttt tacaacagcg agtatgggga gctgtcggag ccaagcgagg aggaccactg
     4081 ctccccgtct gcccgcgtga ctttcttcac agacaacagc tacggagggg gaggttctgg
     4141 cggaggagga tcgggtggtg gtggttcagt gagcaagggc gaggagctgt tcaccggggt
     4201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     4261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4321 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     4381 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4741 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     4801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4861 cggcatggac gagctgtaca agtagggcgc gccgaattcg atatcaagct tatcgataat
     4921 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4981 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5041 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5101 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5161 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5221 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5281 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5341 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5401 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5461 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     5521 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     5581 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     5641 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5701 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     5761 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     5821 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     5881 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     5941 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     6001 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     6061 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6121 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6181 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     6241 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6301 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6361 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6421 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6481 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6961 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7201 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7261 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7321 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     7381 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     7441 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     7501 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     7561 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     7621 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     7681 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     7741 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     7801 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7861 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8041 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8101 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8161 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8221 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8281 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8881 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10201 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    10261 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    10321 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    10381 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    10441 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
    10501 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    10561 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    10621 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    10681 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    10741 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    10801 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    10861 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    10921 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    10981 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    11041 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    11161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    11221 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    11281 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    11341 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    11401 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    11461 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    11521 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    11581 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    11641 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    11701 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
    11761 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    11821 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    11881 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    11941 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    12001 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    12061 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    12121 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    12181 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    12241 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    12301 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    12361 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    12421 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    12481 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    12541 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
    12601 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
    12661 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
    12721 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
    12781 tttcgggttt attacaggga cagcagagat ccagtttggt taattaaccc gtgtcggctc
    12841 cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
    12901 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
    12961 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
    13021 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
    13081 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
    13141 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
    13201 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
    13261 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
    13321 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
    13381 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
    13441 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
    13501 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
    13561 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
    13621 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
    13681 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
    13741 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
    13801 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
    13861 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
    13921 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
    13981 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
    14041 tttggctttt ttgttagac
//
