LOCUS       sequence-231732-       12652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in 
            lentiviral vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12652)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 12652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-hTie1-FLAG
FEATURES             Location/Qualifiers
     source          1..12652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..1235
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1072..1091
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1264..1273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1807..1818)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4687..4710
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4746..5334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4799..4819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5217..5228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5337..5353)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5338..5354)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5859..6039
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6065..6082)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6071..6295
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6341..6769
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6428..6447)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6638..6659
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6778..6798)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6783..7112
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6963..7098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7025..7044
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7160..7207
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7226..7600
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7730..7851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7767..7786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7821..7840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7900..7916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7900..7916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7913..7935)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7924..7940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7948..7954,7955..7972,7973..7978))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7993..8014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8131..8148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8302..8890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8382..8401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9061..9852,9853..9921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9684..9703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9922..10026)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10101..10120)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10292..10671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10673..10871
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10826..10846
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10889..11069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11116..11241
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11734..11967
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12152..12196
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12494..12611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taacccgtgt cggctccaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
       61 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
      121 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
      181 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
      241 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
      301 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
      361 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
      421 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
      481 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
      541 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
      601 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
      661 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
      721 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
      781 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
      841 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
      901 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
      961 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     1021 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     1081 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     1141 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     1201 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtcgactct
     1261 agagccacca tggtctggcg ggtgccccct ttcttgctcc ccatcctctt cttggcttct
     1321 catgtgggcg cggcggtgga cctgacgctg ctggccaacc tgcggctcac ggacccccag
     1381 cgcttcttcc tgacttgcgt gtctggggag gccggggcgg ggaggggctc ggacgcctgg
     1441 ggcccgcccc tgctgctgga gaaggacgac cgtatcgtgc gcaccccgcc cgggccaccc
     1501 ctgcgcctgg cgcgcaacgg ttcgcaccag gtcacgcttc gcggcttctc caagccctcg
     1561 gacctcgtgg gcgtcttctc ctgcgtgggc ggtgctgggg cgcggcgcac gcgcgtcatc
     1621 tacgtgcaca acagccctgg agcccacctg cttccagaca aggtcacaca cactgtgaac
     1681 aaaggtgaca ccgctgtact ttctgcacgt gtgcacaagg agaagcagac agacgtgatc
     1741 tggaagagca acggatccta cttctacacc ctggactggc atgaagccca ggatgggcgg
     1801 ttcctgctgc agctcccaaa tgtgcagcca ccatcgagcg gcatctacag tgccacttac
     1861 ctggaagcca gccccctggg cagcgccttc tttcggctca tcgtgcgggg ttgtggggct
     1921 gggcgctggg ggccaggctg taccaaggag tgcccaggtt gcctacatgg aggtgtctgc
     1981 cacgaccatg acggcgaatg tgtatgcccc cctggcttca ctggcacccg ctgtgaacag
     2041 gcctgcagag agggccgttt tgggcagagc tgccaggagc agtgcccagg catatcaggc
     2101 tgccggggcc tcaccttctg cctcccagac ccctatggct gctcttgtgg atctggctgg
     2161 agaggaagcc agtgccaaga agcttgtgcc cctggtcatt ttggggctga ttgccgactc
     2221 cagtgccagt gtcagaatgg tggcacttgt gaccggttca gtggttgtgt ctgcccctct
     2281 gggtggcatg gagtgcactg tgagaagtca gaccggatcc cccagatcct caacatggcc
     2341 tcagaactgg agttcaactt agagacgatg ccccggatca actgtgcagc tgcagggaac
     2401 cccttccccg tgcggggcag catagagcta cgcaagccag acggcactgt gctcctgtcc
     2461 accaaggcca ttgtggagcc agagaagacc acagctgagt tcgaggtgcc ccgcttggtt
     2521 cttgcggaca gtgggttctg ggagtgccgt gtgtccacat ctggcggcca agacagccgg
     2581 cgcttcaagg tcaatgtgaa agtgcccccc gtgcccctgg ctgcacctcg gctcctgacc
     2641 aagcagagcc gccagcttgt ggtctccccg ctggtctcgt tctctgggga tggacccatc
     2701 tccactgtcc gcctgcacta ccggccccag gacagtacca tggactggtc gaccattgtg
     2761 gtggacccca gtgagaacgt gacgttaatg aacctgaggc caaagacagg atacagtgtt
     2821 cgtgtgcagc tgagccggcc aggggaagga ggagaggggg cctgggggcc tcccaccctc
     2881 atgaccacag actgtcctga gcctttgttg cagccgtggt tggagggctg gcatgtggaa
     2941 ggcactgacc ggctgcgagt gagctggtcc ttgcccttgg tgcccgggcc actggtgggc
     3001 gacggtttcc tgctgcgcct gtgggacggg acacgggggc aggagcggcg ggagaacgtc
     3061 tcatcccccc aggcccgcac tgccctcctg acgggactca cgcctggcac ccactaccag
     3121 ctggatgtgc agctctacca ctgcaccctc ctgggcccgg cctcgccccc tgcacacgtg
     3181 cttctgcccc ccagtgggcc tccagccccc cgacacctcc acgcccaggc cctctcagac
     3241 tccgagatcc agctgacatg gaagcacccg gaggctctgc ctgggccaat atccaagtac
     3301 gttgtggagg tgcaggtggc tgggggtgca ggagacccac tgtggataga cgtggacagg
     3361 cctgaggaga caagcaccat catccgtggc ctcaacgcca gcacgcgcta cctcttccgc
     3421 atgcgggcca gcattcaggg gctcggggac tggagcaaca cagtagaaga gtccaccctg
     3481 ggcaacgggc tgcaggctga gggcccagtc caagagagcc gggcagctga agagggcctg
     3541 gatcagcagc tgatcctggc ggtggtgggc tccgtgtctg ccacctgcct caccatcctg
     3601 gccgcccttt taaccctggt gtgcatccgc agaagctgcc tgcatcggag acgcaccttc
     3661 acctaccagt caggctcggg cgaggagacc atcctgcagt tcagctcagg gaccttgaca
     3721 cttacccggc ggccaaaact gcagcccgag cccctgagct acccagtgct agagtgggag
     3781 gacatcacct ttgaggacct catcggggag gggaacttcg gccaggtcat ccgggccatg
     3841 atcaagaagg acgggctgaa gatgaacgca gccatcaaaa tgctgaaaga gtatgcctct
     3901 gaaaatgacc atcgtgactt tgcgggagaa ctggaagttc tgtgcaaatt ggggcatcac
     3961 cccaacatca tcaacctcct gggggcctgt aagaaccgag gttacttgta tatcgctatt
     4021 gaatatgccc cctacgggaa cctgctagat tttctgcgga aaagccgggt cctagagact
     4081 gacccagctt ttgctcgaga gcatgggaca gcctctaccc ttagctcccg gcagctgctg
     4141 cgtttcgcca gtgatgcggc caatggcatg cagtacctga gtgagaagca gttcatccac
     4201 agggacctgg ctgcccggaa cgtgctggtc ggagagaacc tagcctccaa gattgcagac
     4261 ttcggccttt ctcggggaga ggaggtttat gtgaagaaga cgatggggcg tctccctgtg
     4321 cgctggatgg ccattgagtc cctgaactac agtgtctata ccaccaagag tgatgtctgg
     4381 tcctttggag tccttctttg ggagatagtg agccttggag gtacacccta ctgtggcatg
     4441 acctgtgccg agctctatga aaagctgccc cagggctacc gcatggagca gcctcgaaac
     4501 tgtgacgatg aagtgtacga gctgatgcgt cagtgctggc gggaccgtcc ctatgagcga
     4561 cccccctttg cccagattgc gctacagcta ggccgcatgc tggaagccag gaaggcctat
     4621 gtgaacatgt cgctgtttga gaacttcact tacgcgggca ttgatgccac agctgaggag
     4681 gccggtgact acaaagacga tgacgacaag tgaggcgcgc cgaattcgat atcaagctta
     4741 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5341 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5401 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5461 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5521 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5581 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5641 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5701 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5761 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     5821 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     5881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     6061 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6121 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6181 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6241 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6301 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6541 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6661 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6781 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6841 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6901 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6961 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7021 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7081 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7141 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7201 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7261 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7321 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7381 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7441 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7501 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7561 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7621 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7681 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7741 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7801 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7861 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7921 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7981 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8041 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8101 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10021 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    10081 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    10141 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    10201 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    10261 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
    10321 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    10381 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    10441 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    10501 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    10561 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    10621 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    10681 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    10741 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    10801 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    10861 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10921 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10981 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    11041 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    11101 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    11161 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    11221 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    11281 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    11341 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    11401 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11461 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11521 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    11581 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    11641 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    11701 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    11761 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    11821 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    11881 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    11941 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    12001 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    12061 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    12121 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    12181 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    12241 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    12301 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    12361 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    12421 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
    12481 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    12541 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    12601 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta at
//
