LOCUS       sequence-231733-       11515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes FLAG-tagged extracellular domain of human Tie1 gene 
            cloned in lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11515)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-hTie1-ECD-FLAG
FEATURES             Location/Qualifiers
     source          1..11515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      451..460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(994..1005)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2737..2760
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2796..3384
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2849..2869)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3267..3278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3387..3403)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3388..3404)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3909..4089
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4115..4132)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4121..4345
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4391..4819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4478..4497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4688..4709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4828..4848)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4833..5162
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5013..5148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5075..5094
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5210..5257
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5276..5650
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5780..5901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5817..5836)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5871..5890
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5950..5966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5950..5966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5963..5985)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5974..5990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5998..6004,6005..6022,6023..6028))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6043..6064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6181..6198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6352..6940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6432..6451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7111..7902,7903..7971))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7734..7753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7972..8076)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8151..8170)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8342..8721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8723..8921
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8876..8896
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8939..9119
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9166..9291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9784..10017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10202..10246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10544..10661
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10726..11515,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcttg ggctgcaggt cgactctaga gccaccatgg tctggcgggt gccccctttc
      481 ttgctcccca tcctcttctt ggcttctcat gtgggcgcgg cggtggacct gacgctgctg
      541 gccaacctgc ggctcacgga cccccagcgc ttcttcctga cttgcgtgtc tggggaggcc
      601 ggggcgggga ggggctcgga cgcctggggc ccgcccctgc tgctggagaa ggacgaccgt
      661 atcgtgcgca ccccgcccgg gccacccctg cgcctggcgc gcaacggttc gcaccaggtc
      721 acgcttcgcg gcttctccaa gccctcggac ctcgtgggcg tcttctcctg cgtgggcggt
      781 gctggggcgc ggcgcacgcg cgtcatctac gtgcacaaca gccctggagc ccacctgctt
      841 ccagacaagg tcacacacac tgtgaacaaa ggtgacaccg ctgtactttc tgcacgtgtg
      901 cacaaggaga agcagacaga cgtgatctgg aagagcaacg gatcctactt ctacaccctg
      961 gactggcatg aagcccagga tgggcggttc ctgctgcagc tcccaaatgt gcagccacca
     1021 tcgagcggca tctacagtgc cacttacctg gaagccagcc ccctgggcag cgccttcttt
     1081 cggctcatcg tgcggggttg tggggctggg cgctgggggc caggctgtac caaggagtgc
     1141 ccaggttgcc tacatggagg tgtctgccac gaccatgacg gcgaatgtgt atgcccccct
     1201 ggcttcactg gcacccgctg tgaacaggcc tgcagagagg gccgttttgg gcagagctgc
     1261 caggagcagt gcccaggcat atcaggctgc cggggcctca ccttctgcct cccagacccc
     1321 tatggctgct cttgtggatc tggctggaga ggaagccagt gccaagaagc ttgtgcccct
     1381 ggtcattttg gggctgattg ccgactccag tgccagtgtc agaatggtgg cacttgtgac
     1441 cggttcagtg gttgtgtctg cccctctggg tggcatggag tgcactgtga gaagtcagac
     1501 cggatccccc agatcctcaa catggcctca gaactggagt tcaacttaga gacgatgccc
     1561 cggatcaact gtgcagctgc agggaacccc ttccccgtgc ggggcagcat agagctacgc
     1621 aagccagacg gcactgtgct cctgtccacc aaggccattg tggagccaga gaagaccaca
     1681 gctgagttcg aggtgccccg cttggttctt gcggacagtg ggttctggga gtgccgtgtg
     1741 tccacatctg gcggccaaga cagccggcgc ttcaaggtca atgtgaaagt gccccccgtg
     1801 cccctggctg cacctcggct cctgaccaag cagagccgcc agcttgtggt ctccccgctg
     1861 gtctcgttct ctggggatgg acccatctcc actgtccgcc tgcactaccg gccccaggac
     1921 agtaccatgg actggtcgac cattgtggtg gaccccagtg agaacgtgac gttaatgaac
     1981 ctgaggccaa agacaggata cagtgttcgt gtgcagctga gccggccagg ggaaggagga
     2041 gagggggcct gggggcctcc caccctcatg accacagact gtcctgagcc tttgttgcag
     2101 ccgtggttgg agggctggca tgtggaaggc actgaccggc tgcgagtgag ctggtccttg
     2161 cccttggtgc ccgggccact ggtgggcgac ggtttcctgc tgcgcctgtg ggacgggaca
     2221 cgggggcagg agcggcggga gaacgtctca tccccccagg cccgcactgc cctcctgacg
     2281 ggactcacgc ctggcaccca ctaccagctg gatgtgcagc tctaccactg caccctcctg
     2341 ggcccggcct cgccccctgc acacgtgctt ctgcccccca gtgggcctcc agccccccga
     2401 cacctccacg cccaggccct ctcagactcc gagatccagc tgacatggaa gcacccggag
     2461 gctctgcctg ggccaatatc caagtacgtt gtggaggtgc aggtggctgg gggtgcagga
     2521 gacccactgt ggatagacgt ggacaggcct gaggagacaa gcaccatcat ccgtggcctc
     2581 aacgccagca cgcgctacct cttccgcatg cgggccagca ttcaggggct cggggactgg
     2641 agcaacacag tagaagagtc caccctgggc aacgggctgc aggctgaggg cccagtccaa
     2701 gagagccggg cagctgaaga gggcctggat cagggtgact acaaagacga tgacgacaag
     2761 tgaggcgcgc cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     2821 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     2881 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2941 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3001 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3061 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3121 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3181 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3241 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3301 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3361 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     3421 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     3481 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     3541 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3601 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     3661 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     3721 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     3781 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     3841 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     3901 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3961 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4021 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4081 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4141 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4201 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4261 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4321 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4381 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4441 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4501 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4561 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4621 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4681 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4741 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4801 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4861 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4921 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4981 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5041 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5101 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5161 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     5221 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     5281 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     5341 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     5401 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     5461 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     5521 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     5581 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     5641 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5701 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5761 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5821 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5881 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8101 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     8161 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     8221 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     8281 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     8341 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     8401 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8461 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     8521 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     8581 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     8641 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8701 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     8761 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8821 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8881 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9181 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9241 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9301 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9361 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9421 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9481 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9541 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9601 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9661 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9721 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9781 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9841 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9901 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9961 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10021 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10081 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10141 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10201 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10261 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10321 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10381 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10441 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
    10501 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
    10561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10681 gcagagatcc agtttggtta attaacccgt gtcggctcca gatctggcct ccgcgccggg
    10741 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
    10801 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
    10861 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
    10921 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
    10981 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
    11041 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
    11101 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
    11161 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
    11221 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
    11281 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
    11341 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
    11401 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
    11461 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtg
//
