LOCUS       sequence-231737-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of miR-124 in eurkaryotic cells, encoded in a human 
            beta-globin intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: PB09-TRE-beta-globin-miR-124-EF1a-GFP
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        252..630
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    296..314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    332..350
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    368..386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    404..422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    440..458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    476..494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     539..563
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1908..1910,1911..1913,1914..2627)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1908..1910,1911..1913,1914..2624)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1953..1974)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2214..2233)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2561..2582
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2655..2672)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2661..2868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2914..3125
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3138..3406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3440..4183
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4184..4237
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4238..4837
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4238..4257)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4734..4754
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4876..4997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4913..4932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4967..4986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5759..5793
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6284..6300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6284..6300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6297..6319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6308..6324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6332..6338,6339..6356,6357..6362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6377..6398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6515..6532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6686..7274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6766..6785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7445..8236,8237..8305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8068..8087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8306..8410)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8436..8891)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8573..8594)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8785..8804
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9018..9040
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9032..9049
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9033..9049
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9179..9241
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
       61 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
      121 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
      181 ctgcagacac ctggggggat acggggaaaa ggcctccacg gccactagtc tttcgtcttc
      241 aagaattcct cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct
      301 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
      361 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
      421 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
      481 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
      541 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
      601 actttccgta ccacttccta ccctcgtaaa ggtctagagc tagcgaattc gaatttaaat
      661 cggatccgta cacatattga ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg
      721 ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt
      781 tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac
      841 agtgataatt tctgggttaa ggcaatagcc gattagttct cgaggattcg actgatcgat
      901 ccagtaccga ggagatctgc gccgcgatcg cgcaggaaaa agcctggatg cgaaaggatg
      961 ggggagaaca aagagccttt ggaagacgtc gctgttatct cattgtctgt gtgattgggg
     1021 gagctgcggc ggggaggatg ctgtggtccc ttcctccggc gttccccacc cccatccctc
     1081 tccccgctgt cagtgcgcac gcacacgcgc cgctttttat ttctttttcc tggttttctt
     1141 attccatctt ctacccaccc ctcttccttt ctttcacctt tccttccttc cttcctcctt
     1201 tccttcctca ggagaaaggc ctctctctcc gtgttcacag cggaccttga tttaaatgtc
     1261 catacaatta aggcacgcgg tgaatgccaa gaatggggct ggctgagcac cgtgggtcgg
     1321 cgagggcccg ccaaggaagg agcgaccgac cgagccaggc gccctccgca gacctccgcg
     1381 cagcggccgc gggcgcgagg ggaggggtct ggagctccct ccggctgcct gtcccgcacc
     1441 ggagcccgtg gggtggggag gtgtgcagcc tgtgacagac aggggcttag agatgcaaac
     1501 agactcaggg agagaaacag aagctgattc tgtgacagaa gcagatctgt gcagcacaga
     1561 tgcggtgtgc gtggggaacg cgtacgcggc cgctcgagca gaaactcatc tcagataagt
     1621 cgctagctcg agcttttgga gaatatttct gcatataaat atttctgcat ataaattgta
     1681 actgatgtaa gaggtttcat attgctaata gcagctacaa tccagctacc attctgcttt
     1741 tattttatgg ttgggataag gctggattat tctgagtcca agctaggccc ttttgctaat
     1801 catgttcata cctcttatct tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct
     1861 ggcccatcac tttggcaaag cacgtgagat ctgaattctg acacaccatg gtgagcaagg
     1921 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1981 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2041 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2101 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2161 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2221 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2281 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2341 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2401 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2461 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2521 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2581 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgagaa ttctaactag
     2641 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2701 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2761 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2821 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggaca gctgaatcta
     2881 agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     2941 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
     3001 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     3061 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     3121 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     3181 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     3241 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     3301 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     3361 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatctaag ctgtgaccgg
     3421 cgcctactct agagccgcca tgtctagact ggacaagagc aaagtcataa actctgctct
     3481 ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     3541 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     3601 cctgccaatc gagatgctgg acaggcatca tacccactcc tgccccctgg aaggcgagtc
     3661 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     3721 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     3781 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     3841 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     3901 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga aacaagcaat
     3961 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     4021 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     4081 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     4141 tgctgacgct cttgacgatt ttgaccttga catgctcccc ggggagggca gaggaagtct
     4201 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     4261 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4321 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4381 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4441 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4501 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4561 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4621 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4681 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4741 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4801 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta
     4861 caaaatttgt gggttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4981 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag
     5041 aagctatctg gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt
     5101 gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt
     5161 cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac
     5221 tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg
     5281 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     5341 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     5401 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     5461 gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt
     5521 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     5581 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     5641 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     5701 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     5761 tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc
     5821 gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag
     5881 gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat
     5941 gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt
     6001 gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg
     6061 tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac
     6121 aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg
     6181 ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg
     6241 ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg
     6301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8401 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     8461 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8521 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8581 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8641 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8701 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8761 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8821 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8881 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     8941 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     9001 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc
     9061 gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta
     9121 cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc tagattaacc
     9181 ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa attgacgcat
     9241 gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta
     9301 ttatatttac acttacatac taataataaa ttcaacaaac aatttattta tgtttattta
     9361 tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatgagg
     9421 gacagccccc ccccaaag
//
