LOCUS       sequence-231740-        6487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6487)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..6487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    102..149
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      478..1066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     967..986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1241..1544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1545..1748
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1699..1719
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3617..3626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3623..4333
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3768..3786)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    4455..4576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4492..4511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4546..4565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4583..5038)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4720..4741)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4932..4951
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5065..5169
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5171..5528
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5171..5362
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5194..5214)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5379..5514
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5563..6357
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5563..6357
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5617..5636)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6227..6246
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
       61 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
      121 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
      181 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
      241 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
      301 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
      361 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
      421 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
      481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      601 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
      661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1141 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
     1201 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1261 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1321 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1381 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1441 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1501 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1561 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1621 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1681 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1741 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagac
     1801 cgccatggaa gggaagtggt tgctgtgtat gttactggtg cttggaactg ctattgttga
     1861 ggctcatgat ggacatgatg atgatgtgat tgatattgag gatgaccttg acgatgtcat
     1921 tgaagaggta gaagactcaa aaccagatac cactgctcct ccttcatctc ccaaggttac
     1981 ttacaaagct ccagttccaa caggggaagt atattttgct gattcttttg acagaggaac
     2041 tctgtcaggg tggattttat ccaaagccaa gaaagacgat accgatgatg aaattgccaa
     2101 atatgatgga aagtgggagg tagaggaaat gaaggagtca aagcttccag gtgataaagg
     2161 acttgtgttg atgtctcggg ccaagcatca tgccatctct gctaaactga acaagccctt
     2221 cctgtttgac accaagcctc tcattgttca gtatgaggtt aatttccaaa atggaataga
     2281 atgtggtggt gcctatgtga aactgctttc taaaacacca gaactcaacc tggatcagtt
     2341 ccatgacaag accccttata cgattatgtt tggtccagat aaatgtggag aggactataa
     2401 actgcacttc atcttccgac acaaaaaccc caaaacgggt atctatgaag aaaaacatgc
     2461 taagaggcca gatgcagatc tgaagaccta ttttactgat aagaaaacac atctttacac
     2521 actaatcttg aatccagata atagttttga aatactggtt gaccaatctg tggtgaatag
     2581 tggaaatctg ctcaatgaca tgactcctcc tgtaaatcct tcacgtgaaa ttgaggaccc
     2641 agaagaccgg aagcccgagg attgggatga aagaccaaaa atcccagatc cagaagctgt
     2701 caagccagat gactgggatg aagatgcccc tgctaagatt ccagatgaag aggccacaaa
     2761 acccgaaggc tggttagatg atgagcctga gtacgtacct gatccagacg cagagaaacc
     2821 tgaggattgg gatgaagaca tggatggaga atgggaggct cctcagattg ccaaccctag
     2881 atgtgagtca gctcctggat gtggtgtctg gcagcgacct gtgattgaca accccaatta
     2941 taaaggcaaa tggaagcctc ctatgattga caatcccagt taccagggaa tctggaaacc
     3001 caggaaaata ccaaatccag atttctttga agatctggaa cctttcagaa tgactccttt
     3061 tagtgctatt ggtttggagc tgtggtccat gacctctgac attttttttg acaactttat
     3121 catttgtgct gatcgaagaa tagttgatga ttgggccaat gatggatggg gcctgaagaa
     3181 agctgctgat ggggctgctg agccaggcgt tgtggggcag atgaacgagg cagctgaaga
     3241 gcgcccgtgg ctgtgggtag tctatattct aactgtagcc cttcctgtgt tcctggttat
     3301 cctcttctgc tgttctggaa agaaacagac cagtggtatg gagtataaga aaactgatgc
     3361 acctcaaccg gatgtgaagg aagaggaaga agagaaggaa gaggaaaagg acaagggaga
     3421 tgaggaggag gaaggagaag agaaacttga agagaaacag aaaagtgatg ctgaagaaga
     3481 tggtggcact gtcagtcaag aggaggaaga cagaaaacct aaagcagagg aggatgaaat
     3541 tttgaacaga tcaccaagaa acagaaagcc acgaagagag gggggtgcgt caggcggcag
     3601 cggatctgga ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
     3661 taaggagttc atgcgcttca aggtgcacat ggaggggtcc gtgaacggcc acgtgttcga
     3721 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3781 gaccaagggc ggccccctgc ccttcacctg ggacatcctg agccctcagt tcatgtacgg
     3841 ctccaatgcc tacgtgaagc accccgccga catccccgac tactttaagc tgtccttccc
     3901 cgagggcttc aagtgggagc gcgtgatgaa attcgaggac ggcggcgtgg tgaccgtgac
     3961 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     4021 cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg ccctctccga
     4081 gcggatgtac cccgaggacg gcgccctgaa gggcgaggtc aagcccagag tgaagctgaa
     4141 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     4201 gctgcccggc gcctacaacg tcaaccgcaa gctggacatc accagccaca acgaggacta
     4261 caccatcgtg gagcagtacg agagagccga gggccgccac tccaccggcg gcatggacga
     4321 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     4381 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4441 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4561 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     4621 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4681 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4741 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4801 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4861 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4921 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4981 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     5041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5161 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     5221 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5281 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5341 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5401 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5461 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5521 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5581 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5641 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5701 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     5761 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5821 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5881 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5941 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6001 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6061 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     6121 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6181 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6241 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6301 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6361 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6421 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6481 ggatgat
//
