LOCUS       sequence-231749-        6932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Meyers S, Lenny N, Hiebert SW
  TITLE     The t(8;21) fusion protein interferes with AML-1B-dependent 
            transcriptional activation.
  JOURNAL   Mol Cell Biol. 1995 Apr . 15(4):1974-82.
  PUBMED    7891692
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1995 Apr . 15(4):1974-82."
COMMENT     Sequence Label: pCMV-AML1-ETO
FEATURES             Location/Qualifiers
     source          1..6932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     833..849
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     833..849
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    3133..3755
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3332..3351)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3779..3799)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3784..4113
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3964..4099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4151..4170)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4152..4170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4184..4200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4184..4200)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4197..4219)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4208..4224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4232..4238,4239..4256,4257..4262))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4277..4298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4415..4432)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4586..5174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4666..4685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5345..6136,6137..6205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5968..5987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6206..6310)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6337..6792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6424..6443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6634..6655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(6918..6932,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6932,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attcagatct ggtaccacgc gtatcgataa gcttgcatgc ctgcaggtcg actctagaac
      841 tagtggatcc gttgtgatgc gtatccccgt agatgccagc acgagccgcc gcttcacgcc
      901 gccttccacc gcgctgagcc caggcaagat gagcgaggcg ttgccgctgg gcgccccgga
      961 cgccggcgct gccctggccg gcaagctgag gagcggcgac cgcagcatgg tggaggtgct
     1021 ggccgaccac ccgggcgagc tggtgcgcac cgacagcccc aacttcctct gctccgtgct
     1081 gcctacgcac tggcgctgca acaagaccct gcccatcgct ttcaaggtgg tggccctagg
     1141 ggatgttcca gatggcactc tggtcactgt gatggctggc aatgatgaaa actactcggc
     1201 tgagctgaga aatgctaccg cagccatgaa gaaccaggtt gcaagattta atgacctcag
     1261 gtttgtcggt cgaagtggaa gagggaaaag cttcactctg accatcactg tcttcacaaa
     1321 cccaccgcaa gtcgccacct accacagagc catcaaaatc acagtggatg ggccccgaga
     1381 acctcgaaat cgtactgaga agcactccac aatgccagac tcacctgtgg atgtgaagac
     1441 gcaatctagg ctgactcctc caacaatgcc acctccccca actactcaag gagctccaag
     1501 aaccagttca tttacaccga caacgttaac taatggcacg agccattctc ctacagcctt
     1561 gaatggcgcc ccctcaccac ccaatggctt cagcaatggg ccttcctctt cttcctcctc
     1621 ctctctggct aatcaacagc tgcccccagc ctgtggtgcc aggcaactca gcaagctgaa
     1681 aaggttcctt actaccctgc agcagtttgg caatgacatt tcacccgaga taggagaaag
     1741 agttcgcacc ctcgttctgg gactagtgaa ctccactttg acaattgaag aatttcattc
     1801 caaactgcaa gaagctacta acttcccact gagacctttt gtcatcccat ttttgaaggc
     1861 caacttgccc ctgctgcagc gtgagctcct ccactgcgca agactggcca aacagaaccc
     1921 tgcccagtac ctcgcccagc atgaacagct gcttctggat gccagcacca cctcacctgt
     1981 tgactcctca gagctgcttc tcgatgtgaa cgaaaacggg aagaggcgaa ctccagacag
     2041 aaccaaagaa aatggctttg acagagagcc tttgcactca gaacatccaa gcaagcgacc
     2101 atgcactatt agcccaggcc agcggtacag tccaaataac ggcttatcct accagcccaa
     2161 tggcctgcct caccctaccc cacctccacc tcagcattac cgtttggatg atatggccat
     2221 tgcccaccac tacagggact cctatcgaca ccccagccac agggacctca gggacagaaa
     2281 cagacctatg gggttgcatg gcacacgtca agaagaaatg attgatcaca gactaacaga
     2341 cagagaatgg gcagaagagt ggaaacatct tgaccatctg ttaaactgca taatggacat
     2401 ggtagaaaaa acaaggcgat ctctcaccgt actaaggcgg tgtcaagaag cagaccggga
     2461 agaattgaat tactggatcc ggcggtacag tgacgccgag gacttaaaaa aaggtggcgg
     2521 cagtagcagc agccactcta ggcagcagag tcccgtcaac ccagacccag ttgcactaga
     2581 cgcgcatcgg gaattccttc acaggcctgc gtctggatac gtgccagagg agatctggaa
     2641 gaaagctgag gaggccgtca atgaggtgaa gcgccaggcg atgacggagc tgcagaaggc
     2701 cgtgtctgag gcggagcgga aagcccacga catgatcaca acagagaggg ccaagatgga
     2761 gcgcacggtc gccgaggcca aacggcaggc ggcggaggac gcactggcag ttatcaatca
     2821 gcaggaggat tcaagcgaga gttgctggaa ttgtggccgt aaagcgagtg aaacctgcag
     2881 tggctgtaac acagcccgat actgtggctc attttgccag cacaaagact gggagaagca
     2941 ccatcacatc tgtggacaga ccctgcaggc ccagcagcag ggagacacac ctgcagtcag
     3001 ctcctctgtc acgcccaaca gcggggctgg gagcccgatg gacacaccac cagcagccac
     3061 tccgaggtca accaccccgg gaaccccttc caccatagag acaacccctc gctctcgact
     3121 ctagaggatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     3181 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     3241 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     3301 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     3361 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     3421 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     3481 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     3541 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     3601 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     3661 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     3721 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg
     3781 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3841 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3901 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3961 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4021 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4081 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag
     4141 aaagttaatt ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg
     4201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4261 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4321 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4381 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     6361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6781 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     6841 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     6901 ggtaacgcca gggttttccc agtcacgacg tt
//
