LOCUS       sequence-231752-        9228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-CDIP1-201
FEATURES             Location/Qualifiers
     source          1..9228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..527
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(63..86)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     460..479
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      568..577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             574..2226
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4272..4393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4283..4302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4337..4356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4638..4995
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4638..4829
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4661..4681)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4846..4981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5040..5049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5046..5978
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6030..6821
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6081..6100)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6691..6710
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6885..6933
                     /note="synthetic polyadenylation signal"
     promoter        7089..7193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7194..7262,7263..8054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7412..7431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8212..8800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8292..8311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8916..9199
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 gtccggcgta gaggatcgag atctaccggg taggggaggc gcttttccca aggcagtctg
       61 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
      121 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
      181 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
      241 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
      301 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
      361 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
      421 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
      481 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
      541 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
      601 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
      661 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
      721 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
      781 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
      841 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
      901 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
      961 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     1021 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     1081 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     1141 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     1201 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     1261 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     1321 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     1381 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     1441 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     1501 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     1561 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     1621 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     1681 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     1741 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     1801 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     1861 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     1921 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     1981 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     2041 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     2101 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     2161 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     2221 gtgtaattct agttgtttaa acgagcggag ctgggactcg ggactccccc gcctgtcagt
     2281 ctggccccct gtgctttgct ccctgcgctc agtggtcact ttcccgctcc cacttggggc
     2341 tgggagccgt gccaccatcc cctagaagtc ctgtcctctt caccctgccc tacctgagcc
     2401 gctgactctt ctggcaaaaa ttctgttggg atttaaggcc aagggtcagt gggtggcagg
     2461 gggctggcaa tgagcttgtg tgttggttgg tctgcttggt gtgtgtgatc gggaagataa
     2521 gctgggaggg gtctcctgct ggggtcctga tgcctctgtt tccaaacaag gtacaggttc
     2581 agtccagact ctttccccct gggaccaaca gcagccagag cagttagcca gttagtcccc
     2641 aggcctgtgg ccacaggcgt ttctgacctg ctgggccgag aatgggtaag ttgtctggag
     2701 tcaggtgggc ccacgtagga cagggtcaca aagcctgggt ttgtttctgg gtactttgcg
     2761 cctctggggt gctagaggtg gggcatggtg gctggaagta aaactgccaa ctctggccct
     2821 cagaactctc aggtatagaa gcccaggatg tctaataccc tgtcccagtg cccgagagct
     2881 gcctggtgtc aggtagagag gacactgtac ctgggtgaat gatcagaccc tggtagctaa
     2941 gaaggaactt gtccctttga gtcagtgtgc agaccccctt tcaggccatg cctctgtgaa
     3001 ccctgtattg ctggggccgg aaggagcccc tgagcctagc cccttcccgt ctgccctgtg
     3061 tcctcactgc gtgtgggtat gacctctgcc tggtggctgg tgtatcccaa ctgggcaaga
     3121 gatggcagag ggtccccctt gtgggtgcgc ttggatgtgc agagccttct ccatggattt
     3181 tcttccctgt aagtgccggg cccctcaccc cagctgacag gctgttgctg tgcctgctca
     3241 cacctgctcc tgcaggcaca ctgggctagg gacgaggaag gagcagccac aagtggtaga
     3301 actgccttgg tggacaccag cctcgccctg tctttatttc ctgaatggtt tgtgaacttg
     3361 ctcacctgga ccactgtatc ctgccactgt ccttcctggt ctcgcactgc cactgcatgg
     3421 cctcctgtca ctgtgaatcg tggcccagtc tcagtttgta gtttctcatt aaattggccc
     3481 tttcactccc ccgccctggg cctctgctct cttgcctggc ttccttcttt tttgagggaa
     3541 agagggtggg gctgcaggca gtctactggc aggacgggag gctgagtcct cagggtctca
     3601 caccctcagt gctgatgcca tgccaactgc ctgggacaac accaacacgt aaggacctaa
     3661 ttaaaccaaa ccagagtcgg gtgtagacca gccctgggat ttccagctgt gactgggcca
     3721 gggcacacgt tggtctcggc agtggctgta aggtcacctt ccttcctctg atgctggttt
     3781 caaccatcta tatatggcat ccacgcatgg gatctgcaag ctggagccct cctacccgca
     3841 ggcttgagca cagcatcatc cagccctggg gaggcgcacc cttaagcaag acagactggg
     3901 ctgaggctga caggcagaag actaacagag cgcagtctgc acacgcaggt tctgggcgac
     3961 ctctggccct ggccatctct gcactaactc atctgaatta tgaaggtggc agtcttggtc
     4021 agtagtttaa agagtttccc tactttttaa ccctttttga aataaaactt ttacaggtag
     4081 tagattccta ttaataaaat tatttaagtt catgtttctc ttttagagaa tgaattgttt
     4141 aaaagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg aaaggaagct
     4201 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ggccgcttcg
     4261 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4321 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4381 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4441 gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca
     4501 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4561 ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
     4621 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4681 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4741 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4981 ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaacca aaggctggag
     5041 ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc
     5101 agtggtgggc tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt
     5161 ccgagaagca cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc
     5221 tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga
     5281 tcggaatggg taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca
     5341 agtacctcac cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc
     5401 acgactgggg ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg
     5461 ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca
     5521 tcgaggagga tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata
     5581 acttcttcgt cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt
     5641 tcgctgccta cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct
     5701 ggcctcgcga gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca
     5761 actacaacgc ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc
     5821 ctgggttctt ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg
     5881 tgaaggtgaa gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca
     5941 tcaagagctt cgtggagcgc gtgctgaaga acgagcagac cggtggtggg agcggaggtg
     6001 gcggatcagg tggcggaggc tccggaggga ttgaacaaga tggattgcac gcaggttctc
     6061 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6121 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6181 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6241 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6301 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6361 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6421 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6481 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6541 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6601 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6661 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6721 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6781 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6841 aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat
     6901 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atcctctttg
     6961 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     7021 gtttctgcgg actggctttc tacgtaatgg tttcttagac gtcaggtggc acttttcggg
     7081 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7201 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7261 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7321 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7381 gttttccaat gatgagcact ttcaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7441 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7501 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7561 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaact atcggaggac
     7621 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7681 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7741 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7801 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8041 ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc
     8101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8761 ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc
     8821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8881 atcaaaaagg atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg
     8941 atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg
     9001 ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc
     9061 gcgattgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     9121 gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     9181 ctgccctcgt agccatcgag ataaaccgca gtactccggc cacgatgc
//
