LOCUS       sequence-231753-       10877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 10877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-CALM1-201
FEATURES             Location/Qualifiers
     source          1..10877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        93..592
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(128..151)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     525..544
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             639..2291
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5986..6107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5997..6016)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6051..6070
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6352..6709
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6352..6543
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6375..6395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6560..6695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6754..6763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6760..7692
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7744..8535
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7795..7814)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8405..8424
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8599..8647
                     /note="synthetic polyadenylation signal"
     promoter        8803..8907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8908..8976,8977..9768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9126..9145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9926..10514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10006..10025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(10630..10877,1..36)
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagta ctccggccac
       61 gatgcgtccg gcgtagagga tcgagatcta ccgggtaggg gaggcgcttt tcccaaggca
      121 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      181 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      241 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
      301 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
      361 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
      421 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
      481 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
      541 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
      601 cccaagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta
      661 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
      721 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
      781 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
      841 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
      901 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
      961 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     1021 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     1081 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     1141 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     1201 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     1261 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     1321 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     1381 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     1441 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     1501 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     1561 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     1621 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     1681 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     1741 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     1801 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     1861 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     1921 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     1981 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     2041 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     2101 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     2161 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     2221 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     2281 tcgccgtgta attctagttg tttaaacgag ctcgagacct actttcaact cctttttccc
     2341 ccctctagaa gaatcaaatt gaatctttta cttacctctt gcaaaaaaaa gaaaaaagaa
     2401 aaaagttcat ttattcattc tgtttctata tagcaaaact gaatgtcaaa agtaccttct
     2461 gtccacacac acaaaatctg catgtattgg ttggtggtcc tgtcccctaa agatcaagct
     2521 acacatcagt tttacaatat aaatacttgt actaccttaa tgataaggac tccttaaagt
     2581 tccatttgct aatgattaat acactgtttg ggctggccag tttttcatgc atgcagcttg
     2641 acgattgagc acagtcaggc ctttgtatta aaaatgaaaa atgaaaaaac aaattcaaaa
     2701 cctattcaaa tgggttctag ttcaatttgt ttagtataaa ttgtcatagc tggtttactg
     2761 aaaacaaaca catttaaaat tggtttacct caggatgacg tgcagaaaaa tgggtgaagg
     2821 ataaaccgtt gagacgtggc cccactggta ggatggtcct cttgtacttc gtgtgctccg
     2881 acccatggtg acgatgacac accctggtgg catgcccgtg tatgttggtt tagcgttgtc
     2941 tgcattgttc tagagtgaaa caggtgtcag gctgtcactg ttcacacaaa tttttaataa
     3001 gaaacattta ccaagggagc atctttggac tctctgtttt taaaaccttc tgaaccatga
     3061 cttggagccg gcagagtagg ctgtggctgt ggacttcagc acaaccatca acattgctgt
     3121 tcaaagaaat tacagtttac gtccattcca agttgtaaat gctagtcttt tttttttttt
     3181 ttccaataaa aagaccatta acttaaagtg gtgttaaatg ctttgtaaag ctgagatcta
     3241 aatggggaca aggcaggtgg aggggaggcc agtgtacatg taaatgccca cagcccagca
     3301 ttgggtttcc ctcccaaggc cccagcacca acctctgagc ccaagacctt gcctgaaaac
     3361 aagcagatac cgattgcttc atcctattta tggacatgta ggtctagttg cattttcact
     3421 ggggggaggg gggaaggtga attatggtaa cttttaatga tctattcagg cagtagagct
     3481 cttaaggaaa aaaaaaaccc actttctctc aagcatgtat ttaggggttg ttctcaattg
     3541 tgctgctgat tacctgtctt atgtaactac ttgagaccat ctgcaagaga catgatttag
     3601 tgtgtctgta attcaatctt cgctgtgtgt ggtagaagca gtagtcactt ttgtaagcca
     3661 gtctcttcat gcctaaaaga cactaccagt cacctttgat tcgcgacttt taatttatga
     3721 ttatacttag cctcctcctc cttttttttt ttttcccaag ttgacttgac tttgcttttt
     3781 tccccccaag tagaactaat gctagcttcc agcttgaaag taaaactcca gtgtggagtg
     3841 aattttgtgt ctaattataa acctgtaacc aaaactcaga catctggtac tggtctttgc
     3901 attgagattg gtccctgtaa aacccccttt aaaagcatat tgcatttagt acagagctct
     3961 tttttgaaat gaaggctgga gatgtgcatt tttcacggtg ttaactggtt gtatcttatt
     4021 agcaaggaga ttggggtttt gagtgtttgc gtgggtggtt tcaatttgcc agggaacagt
     4081 ggcaggctgc tagcaaggca gtgagaagct cttggcagcc aaatgggtgc attcagggct
     4141 gatttataga gacccttggc ttctccttct cctactccct gtctttctgg cattttgtag
     4201 cttgttagat tttctgccag aggggtgggt cagagcagtg gaggggagac atcgcccatg
     4261 tgcttctgct actggtcctt gggctgggtg gttggtagag gagatgttga cactatgagc
     4321 taagggttgg cttttgtaat tacctgaatc tgaaaggaat gcctaaggtt accttggggt
     4381 ttctcttctg gtgagatagg gttcctggtt tgagtaagtt aatgtcctgg atatttcttg
     4441 tggcaggggg tggtcaaaga gcctgattgc tgacccagtc tcaggcctgt ggtcgatgac
     4501 ctctcggtag tttcaaaggg ggctggaggg ggatatttga cttgtttttt cgaaatgtag
     4561 ccttctaacc ctcaagtctt tagaagctgg gtggactctt agtggtcctg cagcgtatcc
     4621 taaaagacta cctttgaaac aggattcttg tatggccagg atcgtgtctg ggaaccagaa
     4681 accctacacc ctccccctcc agggaatgct gagttccagt tttgagcaga ggtgaggcag
     4741 aatccactgt agccttccgc cctggtattt ggggggatga ccagcccagg cgttgggtgt
     4801 tagtctgcat gagtttgtga gaggaaatag ctgggtgtcc tggcagtgcc cttgaagttg
     4861 gttaggacct tcctgtaaac tcttgcccct acttctaact actctataaa tatatacata
     4921 tatttatata taaagtgatt agttgaactg gcatcctgct ttagcctgag acttgccata
     4981 agaaactgct gagtacttgg caaacccttt catagttttg ttctccatct gtttggggta
     5041 ggtgttgagc gaggcaaatg gatctcgata tttcagatgg gcttttgatg cactgttgcc
     5101 aaggaaggct ttttctgatt ttttgacaaa tgaatttttg cacactttca ttggtgtctt
     5161 tcggcaactt acacacattg aaaatgagct attgtacata tttttatatt ctctttataa
     5221 atgcatgtct gattgtactt gtaacaatat tgtaatgaac ggctgtgcag taggcccagc
     5281 gctgctgtgt ctcgtcagag gaatagctta ccacgaaccc ctcagcatac tgggaatctc
     5341 ttcctgaaca acgaatgtaa atttggtcaa gtctactctt ccgttcattc aattatttta
     5401 agcatttgaa ttatttattg tatatcctaa atatatttct cctttggcag tgactagatt
     5461 tccactaatg tgtcttaatc tatccctcca gctggcagtt actgtttttt taatcccctg
     5521 aagttgtcct gtaggagaca gaaattcttt gctgtctgta tcccttggag taagaaggta
     5581 gtggcatggg tggagtgtgt gttctttctc caaatctatt atgatgttta ttaaacactt
     5641 ctgtagcaaa gatggtggta gttcttttgt tactgaagtt gcccttcacc atggctattt
     5701 gaaaaggaga tgtacttgga cgtttctgta aatcttgaga taaactgttt ggagatttaa
     5761 ccacctctct gatgggggac caactctatg gaaattgtaa atacgtttta tttataaacc
     5821 tggcactgta ttcaataaac atttctgcag cctttcaagt cgacctgcag gcatgcaagc
     5881 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     5941 ataactagca taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg
     6001 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6061 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6121 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa
     6181 acctctacaa atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga
     6241 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
     6301 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     6361 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6421 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6481 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6541 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6601 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6661 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc
     6721 ttctgacaca acagtctcga accaaaggct ggagccacca tggcttccaa ggtgtacgac
     6781 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
     6841 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
     6901 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
     6961 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
     7021 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
     7081 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
     7141 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
     7201 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
     7261 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
     7321 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
     7381 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
     7441 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
     7501 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
     7561 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
     7621 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     7681 aagaacgagc agaccggtgg tgggagcgga ggtggcggat caggtggcgg aggctccgga
     7741 gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7801 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7861 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7921 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7981 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     8041 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     8101 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     8161 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     8221 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     8281 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     8341 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     8401 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8461 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8521 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     8581 tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     8641 ttgtgtgaat cgatagcgat aaggatcctc tttgcgcttg cgttttccct tgtccagata
     8701 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgta
     8761 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttcaaa
     9121 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9301 gcggccaact tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac
     9361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaatt cgaaatgacc
     9781 gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10501 aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10621 ttttatagtc cggaaataca ggaacgcacg ctggatggcc cttcgctggg atggtgaaac
    10681 catgaaaaat ggcagcttca gtggattaag tgggggtaat gtggcctgta ccctctggtt
    10741 gcataggtat tcatacggtt aaaatttatc aggcgcgatt gcggcagttt ttcgggtggt
    10801 ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt
    10861 acaattaagg gattatg
//
