LOCUS       sequence-231755-       11508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-GRM4-202
FEATURES             Location/Qualifiers
     source          1..11508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     302..321
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      410..419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             416..2068
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    6394..6515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6405..6424)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6459..6478
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6760..7117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6760..6951
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6783..6803)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      7162..7171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7168..8100
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             8152..8943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8203..8222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8813..8832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9007..9055
                     /note="synthetic polyadenylation signal"
     promoter        9211..9315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9316..9384,9385..10176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9534..9553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10334..10922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10414..10433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11038..11321
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(11378..11508,1..369)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11413..11436)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
       61 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      121 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      181 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
      241 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
      301 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
      361 ggcctttcga cctgcagccc aagcttggca atccggtact gttggtaaag ccaccatgga
      421 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
      481 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
      541 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
      601 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
      661 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
      721 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
      781 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
      841 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
      901 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
      961 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     1021 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     1081 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     1141 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     1201 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     1261 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     1321 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     1381 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     1441 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     1501 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     1561 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     1621 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     1681 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     1741 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     1801 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     1861 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     1921 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     1981 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     2041 caagaagggc ggcaagatcg ccgtgtaatt ctagttgttt aaacgagctc gagtccatgg
     2101 agctgagcag caggaggagg agccgtgacc ctgtggaagg tgcgtcgggc cagggccaca
     2161 cccaagggcc cagctgtctt gcctgcccgt gggcacccac ggacgtggct tggtgctgag
     2221 gatagcagag cccccagcca tcactgctgg cagcctgggc aaaccgggtg agcaacagga
     2281 ggacgagggg ccggggcggt gccaggctac cacaagaacc tgcgtcttgg accattgccc
     2341 ctcccggccc caaaccacag gggctcaggt cgtgtgggcc ccagtgctag atctctccct
     2401 cccttcgtct ctgtctgtgc tgttggcgac ccctctgtct gtctccagcc ctgtctttct
     2461 gttctcttat ctctttgttt caccttttcc ctctctggcg tccccggttg cttgtactct
     2521 tggccttttc tgtgtctcct ttctggctct tgcctccgcc tctctctctc atcctctttg
     2581 tcctcagctc ctcctgcttt cttgggtccc accagtgtca cttttctgcc gttttctttc
     2641 ctgttctcct ctgcttcatt ctcgtccagc cattgctccc ctctccctgc cacccttccc
     2701 cagttcacca aaccttacat gttgcaaaag agaaaaaagg aaaaaaaatc aaaacacaaa
     2761 aaagccaaaa cgaaaacaaa tctcgagtgt gttgccaagt gctgcgtcct cctggtggcc
     2821 tctgtgtgtg tccctgtggc ccgcagcctg cccgcctgcc ccgcccatct gccgtgtgtc
     2881 ttgcccgcct gccccgcccg tctgccgtct gtcttgcccg cctgcccgcc tgcccctcct
     2941 gccgaccaca cggagttcag tgcctgggtg tttggtgatg gttattgacg acaatgtgta
     3001 gcgcatgatt gtttttatac caagaacatt tctaataaaa ataaacacat ggttttgcac
     3061 ccgggctcca catccactga gggtcctgcc atgggaccac aggctcagcc tgcagctgga
     3121 gggcttagac ctagagggaa gcgggaactg ggctctggag acccagggct tgagggctgt
     3181 ggagactgct ccctaggctg ggatctagtg tggtgtggtg aggccttggg catggagggg
     3241 ccagattccc aggtaagggg cagggacatt gcaggaaatt ccaggaatca gcacctagta
     3301 gtcccctaat tagggggtat gctctgtccc ctgccctgca gccctgggag ggtaacattt
     3361 ctgccttgcc tgtcctctgt ctcacacccc tcacacctgg gactgccctt ccacccctgc
     3421 ccccataacc tgtgcctctc tccttccagc caggaagtcc tcttcttgag aagttagctt
     3481 cccgggctgc cagcactcat agccgtcccc tcctgcttgt gttggctcca ggctcgggtg
     3541 ctaagaagat gtgtgtctgt cctggagatc agtgtgttgt tatgtgtcca cgtgggccca
     3601 caagtgcacg gcacaggcat ggccgtgtgg ctgtgttggc tgtgttggct gtgtgtctgt
     3661 gtgcacgtcc agcgcctcca tgcgcatgcg tgcctgtctt gtttgcgtgt ctgatcatct
     3721 gtttgggccc cggtggctca tgcagatgcc tgtctcaggc ccatggcgag tgttcacctc
     3781 agctggcttc cctggcaggt tgggaggtgg gaaacaggag cgcttagggg ctgggctctg
     3841 gctggggtaa attatagagc cagaaacaca atgaggccat aggcagcagc tggagcctgg
     3901 gctgcctgtg ccgtcccctc ctgccctgcc cctgggtcct gcaccccctc ccacctccag
     3961 gctagctgac agcgctatgg agcacagtgg aagggactgg aggaacccta ggcagggggc
     4021 cacgcaggga cagagtatga gagtgtgtgt ataactgagg ctgggacatt gaatcatgcc
     4081 aggtatgtct tctccatcag cccactctta ctcctggcct gggcatctca cacatctgtg
     4141 cataggaaat ctcttcttcc ctggggtctg tgtgcagcac ctagtagatg ctcaataaat
     4201 gtttgtgtga gggaaggaga caggaaagga agtgtctcgc tgatcatctt gcggaatggt
     4261 tcctaagacc tctgcccagg aaagattcca cccagtgctc cagcccggtc aggcagaact
     4321 aggttgccag atcaagggta tctcccaaaa gcttccaggg cagttggggg tgggggggtg
     4381 gggggtaggg atggggaatg cagaagcggg tgcagccagc tctcccccag ggtgactctg
     4441 gcagcacccc catcctgggc accctgcctg ctctgtggct cacgcccctc ctgaagtgac
     4501 tgatgctctg aggcccaagg ctaggtccag ggcagggcct gcaggggttt catgctcagt
     4561 ccaggacttg cctaggtccc cctacatctg tggggccccc atctaggttc taacaggaga
     4621 atcacctctc caagggggat gctgcccctc ggctcccctt ggctctcagg aggggccctc
     4681 agggactacc agtcccctgc cagtgggaag aaatagccct gccctcaggg agcttccagt
     4741 gtgatggggg agatacagca gactgtgtcc caaagtaaaa tgactgttag aatgaggtgg
     4801 gtggaggagg gaagccttgg gtgggtgtga ctttgggcat ctgagcctgg ggtgcagagg
     4861 tgggctctgt gggcctgagg tggacaggag ggaaccaggc cctagcaaga cttttgccag
     4921 ctagacctgc tgcagcagtt gggagggtgg gtgctgctgg agtcctgggt ccatcaccta
     4981 gaaggctcag gccagtgcag ccagggctgg ggcccacagc tggcctgggt gggacctgcc
     5041 ctgatgccca tggcaggagg gacacctggc ccttcacaat tggcttggct gctcaccttt
     5101 gctctcatcc tcaattatta atgactggag aaagctgcta agtatcttca gaatgttaga
     5161 tttcaacaag atggggggtt cagggtccct ggcaccctgg atagggagcc agcggcccct
     5221 agagaccttt gctgtgtgca gggggtatgt gctcaccccc gtggcctcag cctcctcaat
     5281 gtctgaatga aggattgggc tagcagacat cccaccccac agcacacttt ctaaccagca
     5341 ggggaacttc tagacaatag agacgctggg ctccctccag aacactggac ctgaacttct
     5401 ggggggaggg ctgggcacgg gcatatttta aaagctcccc agcagatggg ccgtgtagtc
     5461 aagtgggcca agagtggcac cagactttgg ggcttgtgaa gtcaggaggg agcaacagtg
     5521 cccactcgag cttgcctggg gctcaagccc aaggctgggc tgctgccagc ctgagcagac
     5581 acccaggagc ttccaggcca gctggatgca cagggcacct ttgtggaact cctaggaccc
     5641 tggggagacc cacctcagga gcagagtctc aggtcccttc cggctctgag gggctgttct
     5701 gagctctaat gtcttatggt ctgcccctcc catccttact tctcaggccc tggaggcaga
     5761 ggcatagagc caggcaggac agaggtctca gtgggccaca tgccagctgc ccccacactg
     5821 cctcagcctc caggcctcca aggggtcctg gggagcccct gagaagatgc tgagcctgca
     5881 taaggctggg cgcccctctt tctgacaccc tcactggctc cacggctccc ccttcccatc
     5941 ccaggtttcc atctgcccac tgaacaggga ggggaaactg aggcactccc ctggcactga
     6001 gggctccttc tgtcatcctg cctgccctgg atggtcctgg ctgcccctca gggcttggcc
     6061 ctggcactgt gagcctcaca gggctcagac ccccaccccc aacccagcac taaatggcac
     6121 tcggcaccag aatctcactt cagttggcaa aagcagcaat tagcatgtaa tgaggcttct
     6181 tgctttattt ttaggtaacc tccaaggccc tgcctgtgta attcagcccg ccattgctcg
     6241 gtggataatt aaagcatgtc accatagtcg acctgcaggc atgcaagctg atccggctgc
     6301 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     6361 accccttggg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     6421 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6481 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6541 tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac ctctacaaat
     6601 gtggtaaaat cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     6661 tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc
     6721 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     6781 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgattctt ctgacacaac
     7141 agtctcgaac caaaggctgg agccaccatg gcttccaagg tgtacgaccc cgagcaacgc
     7201 aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac
     7261 tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat
     7321 ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct
     7381 agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg gaatggctca
     7441 tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct gaaccttcca
     7501 aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca ctactcctac
     7561 gagcaccaag acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag
     7621 tcctgggacg agtggcctga catcgaggag gatatcgccc tgatcaagag cgaagagggc
     7681 gagaaaatgg tgcttgagaa taacttcttc gtcgagacca tgctcccaag caagatcatg
     7741 cggaaactgg agcctgagga gttcgctgcc tacctggagc cattcaagga gaagggcgag
     7801 gttagacggc ctaccctctc ctggcctcgc gagatccctc tcgttaaggg aggcaagccc
     7861 gacgtcgtcc agattgtccg caactacaac gcctaccttc gggccagcga cgatctgcct
     7921 aagatgttca tcgagtccga ccctgggttc ttttccaacg ctattgtcga gggagctaag
     7981 aagttcccta acaccgagtt cgtgaaggtg aagggcctcc acttcagcca ggaggacgct
     8041 ccagatgaaa tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag
     8101 accggtggtg ggagcggagg tggcggatca ggtggcggag gctccggagg gattgaacaa
     8161 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     8221 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     8281 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     8341 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     8401 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     8461 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     8521 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     8581 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     8641 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     8701 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8761 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8821 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8881 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8941 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     9001 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     9061 atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     9121 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtaat ggtttcttag
     9181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9481 gagagttttc gccccgaaga acgttttcca atgatgagca ctttcaaagt tctgctatgt
     9541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9721 cttctgacaa ctatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9841 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9901 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9961 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10021 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10081 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10141 gctgagatag gtgcctcact gattaagcat tggtaattcg aaatgaccga ccaagcgacg
    10201 cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10741 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc
    10921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg
    11041 gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg
    11101 cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc ataggtattc
    11161 atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt gttgccattt
    11221 ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga
    11281 ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg cagtactccg
    11341 gccacgatgc gtccggcgta gaggatcgag atctaccggg taggggaggc gcttttccca
    11401 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
    11461 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggct
//
