LOCUS       sequence-231765-        6460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Kelley JB, Talley AM, Spencer A, Gioeli D, Paschal BM
  TITLE     Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha
            family of nuclear import receptors.
  JOURNAL   BMC Cell Biol. 2010 Aug 11;11:63. doi: 10.1186/1471-2121-11-63.
  PUBMED    20701745
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2010-08-11"; volume: "11:63. doi"; pages: " 
            10.1186/1471-2121-11-63"
COMMENT     Sequence Label: pCMVTNT-T7-KPNA5
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        523..726
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     677..697
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     723..747
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..994
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     977..996
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1039..1057
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1112..1121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2802..2923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2813..2832)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2867..2886
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3104..3559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3191..3210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3401..3422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3626..3645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3745..3767
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3805..3823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     mobile_element  3948..4715
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             join(4773..4841,4842..5633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4991..5010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5804..6392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6293..6312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt
       61 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
      121 tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata gtaatcaatt
      181 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      241 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      301 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      361 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      421 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      481 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      541 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      601 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      661 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      721 agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt ttatcacagt
      781 taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagaagt
      841 tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
      901 tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt
      961 cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt caattacagc
     1021 tcttaaggct agagtactta atacgactca ctataggcta gcatttaggt gacactatag
     1081 aatacaagct acttgttctt tttgcactcg agccaccatg gccagcatga ccggcggcca
     1141 gcagatgggc gaattcgatg ccatggctag tccagggaaa gataactata gaatgaaaag
     1201 ttataagaat aaagccctaa atcctcaaga gatgcgtaga cgaagagaag aagaaggaat
     1261 acagcttaga aaacaaaaaa gagaagaaca gttgttcaaa cgcagaaatg tctatttgcc
     1321 cagaaatgat gaatctatgc ttgaaagtcc tatacaggat ccagatatta gttccactgt
     1381 acccattcca gaggaagaag ttgttactac agatatggtt cagatgattt tttctaataa
     1441 tgctgatcaa cagctaacag caacacagaa atttagaaag ctgctttcta aagaacctaa
     1501 tccaccaata gatcaagtta tacagaaacc aggagttgta cagagatttg tgaaatttct
     1561 tgaaagaaat gaaaattgca ctttacaatt tgaagctgca tgggcattaa caaatatagc
     1621 atctggaact tttctgcata ccaaggtagt gattgaaact ggggctgttc cgatttttat
     1681 caaacttctt aattctgaac atgaagatgt tcaggaacag gctgtttggg cacttggtaa
     1741 tattgctggt gacaatgcag aatgcagaga ttttgttttg aattgtgaaa tacttccacc
     1801 tcttttagag ttattaacaa attcaaacag actcacaaca acaagaaatg ccgtgtgggc
     1861 cctctcaaat ttatgtagag gcaaaaaccc tcctccaaac tttagtaagg tttcaccttg
     1921 cttaaatgtc ctgtcacgac tgttgtttag cagtgaccca gatgtgttag cagacgtgtg
     1981 ttgggccctt tcttatctct ccgatggacc caatgataaa attcaagcag tcattgattc
     2041 tggagtctgt cgaagattgg tggaactttt gatgcacaat gattataaag ttgtatcacc
     2101 tgcattaagg gcagttggta atattgtgac tggtgatgat attcaaacac aggtaatttt
     2161 gaattgttct gcattaccct gtctcttaca tttattgagt agcccaaagg agtcaattag
     2221 aaaagaagcc tgctggactg tttctaacat cactgctgga aatagagctc agattcaggc
     2281 tgttatagat gcaaatattt ttcctgtttt gattgagatt cttcagaaag cagagtttcg
     2341 taccagaaaa gaagcagctt gggctataac taatgcaaca tcaggaggta ctccagagca
     2401 aataaggtat ttggtagctt taggctgcat taaaccactt tgtgatcttt tgactgttat
     2461 ggactccaaa atagtccaag tggctttaaa tggacttgaa aatattttac gtcttggaga
     2521 acaagaatct aagcagaatg gaataggcat taatccatac tgtgctctca ttgaagaagc
     2581 atatggtctg gataaaattg agtttttgca aagccatgaa aatcaggaaa tttaccagaa
     2641 ggcatttgat ctgattgaac attactttgg tgtagaagaa gatgacccca gcattgtacc
     2701 tcaggtggat gaaaaccaac aacagtttat atttcagcag caggaagcac caatggatgg
     2761 atttcaactt taacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2821 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2881 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2941 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     3001 ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt aatagcgaag aggcccgcac
     3061 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     3121 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3181 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3241 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3301 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3361 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3541 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3601 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3661 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3721 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3781 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgggt aatgactcca
     3961 acttattgat agtgttttat gttcagataa tgcccgatga ctttgtcatg cagctccacc
     4021 gattttgaga acgacagcga cttccgtccc agccgtgcca ggtgctgcct cagattcagg
     4081 ttatgccgct caattcgctg cgtatatcgc ttgctgatta cgtgcagctt tcccttcagg
     4141 cgggattcat acagcggcca gccatccgtc atccatatca ccacgtcaaa gggtgacagc
     4201 aggctcataa gacgccccag cgtcgccata gtgcgttcac cgaatacgtg cgcaacaacc
     4261 gtcttccgga gactgtcata cgcgtaaaac agccagcgct ggcgcgattt agccccgaca
     4321 tagccccact gttcgtccat ttccgcgcag acgatgacgt cactgcccgg ctgtatgcgc
     4381 gaggttaccg actgcggcct gagtttttta agtgacgtaa aatcgtgttg aggccaacgc
     4441 ccataatgcg ggctgttgcc cggcatccaa cgccattcat ggccatatca atgattttct
     4501 ggtgcgtacc gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt ttacggcagt
     4561 gagagcagag atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag
     4621 tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc
     4681 atcatacact aaatcagtaa gttggcagca tcaccccgct catgagacaa taaccctgat
     4741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4981 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5161 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5941 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6421 tcctggcctt ttgctggcct tttgctcaca tggctcgaca
//
