LOCUS       sequence-231771-        4168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin-S resistance Vector for Phaeodactylum tricornutum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Buck JM, Rio Bartulos C, Gruber A, Kroth PG
  TITLE     Blasticidin-S deaminase, a new selection marker for genetic 
            transformation of the diatom Phaeodactylum tricornutum.
  JOURNAL   PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717/peerj.5884. eCollection 
            2018.
  PUBMED    30488015
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2018-11-14"; volume: "6:e5884. doi"; pages: " 
            10.7717/peerj.5884. eCollection 2018"
COMMENT     Sequence Label: pPTbsr
FEATURES             Location/Qualifiers
     source          1..4168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        137..155
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     137..154
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1958..1977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1959..1977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2064..2081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2235..2823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2315..2334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2994..3785,3786..3854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3617..3636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3855..3959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4027..4045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4083..4105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
       61 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
      121 gtatcataca catacgattt aggtgacact atagaaccag atcccccggg ctgcaggacg
      181 caatggagga ttatcaccgc aaaaatgaac ttcgaaaaaa actttcgagc gaccatggaa
      241 aaggaggatc agattcagat tacaacagtg gattgctctg gtagcaaata tcttctgcta
      301 gattggctca tggtcggttt tggacgttcg aagctcaccg tcaaaagaaa caaaagagaa
      361 gaatgacgtc ttcgtgacgt agaatctacg actgtactcg gatctgggaa atgaattgac
      421 tcacggtctt cttcgagtcc tgttacaggc ccttggtccg aacccccaca cgatttttgc
      481 accaaagatt tgcttcaatt tgctggatgt tttgactgca agatcagctg gcctagcaag
      541 agtgctcgtg ttgcttcgtc gggaatccct acgaatttca gttctgcaca aatttgtctg
      601 ccgtttcgag aattcgatat catcgactaa ttcgagctcg gtacccgggg atcctctaga
      661 gtcgacctgc aggcatgcaa gcttcagaag cgtgctatcg aactcaacca gggacgtgcg
      721 gcacaaatgg gcatccttgc tctcatggtg cacgaacagt tgggagtctc tatccttcct
      781 taaaaattta attttcatta gttgcagtca ctccgctttg gtttcacagt caggaataac
      841 actagctcgt cttcaccatg gatgccaatc tcgcctattc atggtgtata aaagttcaac
      901 atccaaagct agaacttttg gaaagagaaa gaatatccga atagggcacg gcgtgccgta
      961 ttgttggagt ggactagcag aaagtgagga aggcacagga tgagttttct cgagacatac
     1021 cttcagcgtc gtcttcactg tcacagtcaa ctgacagtaa tcgttgatcc ggagagattc
     1081 aaaattcaat ctgtttggac ctggataaga cacaagagcg acatcctgac atgaacgccg
     1141 taaacagcaa atcctggttg aacacgtatc cttttggggg cctcccgcta cgacgctcgc
     1201 tccagctggg gcttccttac tatacacagc gcgcatattt cacggttgcc agatgtcaag
     1261 acaattggtt tatgaaaacg tttaatatct cgcaacaaga ccttgaactt gtcgaagtcg
     1321 caacggaaaa aattacgatg ctttacgaag acaataaaca tcatgtcgga gcagcaattc
     1381 gaacgaaaac gggagaaatt atttcggcag tccatattga agcatacatt ggacgagtca
     1441 cggtctgcgc agaagcaatt gcaattggat cggcagtctc gaatggacaa aaagactttg
     1501 acacgattgt cgcagtccga catccatact cggacgaagt cgatcgatcg attcgagtcg
     1561 tctcgccatg cggaatgtgc cgagaactta tttcggacta cgcaccagac tgctttgtcc
     1621 ttattgaaat gaatggaaaa cttgtcaaaa cgacgattga agaacttatt ccacttaaat
     1681 acacgcgaaa ttgaaaacca ggactgaacc ttccttaaaa atttaatttt cattagttgc
     1741 agtcactccg ctttggtttc acagtcagga ataacactag ctcgtcttca ccatggatgc
     1801 caatctcgcc tattcatggt gtataaaagt tcaacatcca aagctagaac ttttggaaag
     1861 agaaagaata tccgaatagg gcacggcgtg ccgtattgtt ggagtggact agcagaaagt
     1921 gaggaaggca caggatgagt tttctcgagg ccggtctccc tatagtgagt cgtattaatt
     1981 tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2041 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2101 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2161 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2221 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2281 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2341 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2401 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2461 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2521 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2581 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2641 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2701 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2761 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2821 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2881 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2941 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3001 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3061 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3121 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3181 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3241 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3301 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3361 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3421 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3481 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3541 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3601 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3661 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3721 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3781 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3841 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3901 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3961 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4021 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     4081 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4141 agcagacaag cccgtcaggg cgcgtcag
//
