LOCUS       sequence-231777-        5439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse Ret In situ hybridization probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD
  TITLE     Molecular identification of rapidly adapting mechanoreceptors and 
            their developmental dependence on ret signaling.
  JOURNAL   Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003.
  PUBMED    20064391
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.11"; journalName: "Neuron"; date: 
            "2009-12-24- 24"; volume: "64"; issue: "6"; pages: "841-56"
COMMENT     Sequence Label: pBS C-ret
FEATURES             Location/Qualifiers
     source          1..5439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3195..3211)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3195..3211)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3248..3266)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3287..3303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3287..3303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3300..3322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3311..3327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3335..3341,3342..3359,3360..3365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3380..3401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3518..3535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3689..4277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3769..3788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4448..5239,5240..5308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5071..5090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5309..5413)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc
      661 ccctcgagtt tttttttttt tttttttcag aaagcattgg ctcagtattt agcactggct
      721 tcgtgagtga caacatcatg atgggggtag ggtgcaaagg atgtgaaagc agcagtctgg
      781 tgtctgccca gggcccacat ggttttcccg ctgccccaca ctgcacggct gtggacacat
      841 agctggaagt gggattatgg tcggcttccg cgaggcacac accagcccag gagcagcctg
      901 ggggtagccc agcttgcaac gatagcatag ctggggatgc cagctttccc tggcaaaact
      961 gactccctcc ccagatcagg taaatttaaa tatcgaagtc accagtacta tgatttgctt
     1021 cccaatgggc cctgagaatt ctgctctaat ccgcttctcc tgtgtcatcg taagttgctg
     1081 ggctgtgtgg taatgacact tattttcaca tccttttaga aagacatagc tgcaaaatgc
     1141 ctgaatgtat gtaaactaga tgcaaagtca gatatttaca aagaaaaggg ttcggaggag
     1201 gctttggtgt cggtgggatc agaaacactc tttaaaaagc gttacagaga gttgggatgg
     1261 tgcagagagg acagcgttgc tagaatctag taaatgcatg tgaaattcta ccatagagtt
     1321 tgttttcaat ccatgtggaa gggagggagc gcgggagggg agcattgtta cagtccacca
     1381 ggggtgtctc ctcttctgag agcccatcgt catacagcag tgagtccgaa ggtgtggatg
     1441 cagccaggtc caagtagtct ctgctcttga ccatcatctt ctccagatcc ttgctgatgt
     1501 cagcaaacac tggcctcttg tctggctcct gcttccagca ctgcagcatc agacggtaca
     1561 tttcctcgct gcagttgtct ggcctctcca tcctgtggcc tgtcttcaga aggttgaaga
     1621 gtcgttcagg aggaattcca gggtaggggt tgcctcccag ggtcacaatc tcccagagca
     1681 gcactccaaa ggaccacaca tcactttgag tagtatagat gtgatcgaaa agggactcaa
     1741 ttgccatcca cttgacggga atccggccct tgcttttctt cacataggaa tcttcctcat
     1801 aaacatctcg ggacagccca aagtcggaaa tcttcatctt ccgtccctca gccaccaaga
     1861 tgttcctggc agctaagtcc cgatgtacaa gcttcatttc tgccaagtac tgcatacccc
     1921 tcgagatctg ccaggcgaag gagatgaggt cacccatggt cagtaccctt tcatctgggt
     1981 ggtccagcga gctggagttg cggctgcctc caccgctcac ataggcaggc ccaatcttgc
     2041 ggctgtcacg gaggaatccc cgcagggagc catacttggc atactccaca atgagaagaa
     2101 gtggcccatc ctggctgcag gccccataca acttgatgac atgtggatgg ttgacttgtt
     2161 tcagaaggtt gaactcagac agcaggtctc gtaactcact ctgggaggcg ttttctttca
     2221 gcattttcac agccactgtg gtgtatcctg cccggccttt cagacggaag gctgtggcct
     2281 tgacaacttt tccaaactcg ccttctccca gagttttccc aagaactaag ttcttccgag
     2341 gaaattccca ctttggatcc tccgggatct tgaaagagtc cacaggaacc tggttctccg
     2401 tggaatccag tgagggccgg cgggtgcctg aggaggaata actgattggg aagccctggg
     2461 ccggccggca gaaggtcatt tccgctgatg caatgggcgg cttgtgccca tgcttgtggt
     2521 ggtggcagac acagaagatg gacagcagga tggagatgat aagggagaag agggcagctg
     2581 tgatgatcgt gcggcacagc gcatcacaca gtgggccctg gctgtcctct ggctcgcaga
     2641 agcatttctt ctcatcaggg aaacagttgc agatgccgta gcctgcttta atgccctggc
     2701 gctcccctcg ctcgtgtcct ccaacaatgt cggcacggag acagtcctgg gggcaaatgt
     2761 tggcatcccg gctctccaca gcatcacagt ggccgtcggg gcaggtcctg gtactggggg
     2821 agcaggtgga gaagttcctg gtgatccctt taccatctcc ctggcgccac tcgcacctgc
     2881 cagttggaga acccaggcca ccacattcct cacactcggg gcgcctcttg tttactgcac
     2941 aggacttggg gcagcctact tcttcagtaa tggatgtccc ctccacagtg accactagcg
     3001 aagcctgggt ctgtctgcgg gtctgccggt cagtggctac caccgtgtac tgaagcttgg
     3061 tgcactcagg tcgccgcagg gcctctgtgt catttacaaa tagggtcccc gaggtgtcct
     3121 cgggtgaggt gaccacacct agggcagtgc agttgatgct ggaaggcctc gtgccgaatt
     3181 cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct
     3241 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
     3301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5401 taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
