LOCUS       sequence-231782-        7086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Rauch S, He C, Dickinson BC
  TITLE     Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation
            of Single RNAs.
  JOURNAL   J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 
            10.1021/jacs.8b05012. Epub 2018 Sep 18.
  PUBMED    30183280
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.8b05012"; journalName: "J Am Chem Soc"; date: 
            "2018-09-26- 26"; volume: "140"; issue: "38"; pages: "11974-11981"
COMMENT     Sequence Label: Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)
FEATURES             Location/Qualifiers
     source          1..7086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          816..948
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     931..950
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4323..4346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4464..4482)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4519..4640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4530..4549)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4584..4603
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             5039..5833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5093..5112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5703..5722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6005..6593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6085..6104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6709..6992
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
       61 tttatattgg ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tacgggactt tcctaattgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct ggtttagtga accgtcagat cactagaagc tttattgcgg
      721 tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact
      781 taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      841 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      901 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      961 agttcaatta cagctcttaa ggcagatcac tagaagctta cgctaccacc atgaacatcc
     1021 ccgctctggt ggaaaaccag aagaagtact ttggcaccta cagcgtgatg gccatgctga
     1081 acgctcagac cgtgctggac cacatccaga aggtggccga tattgagggc gagcagaacg
     1141 agaacaacga gaatctgtgg tttcaccccg tgatgagcca cctgtacaac gccaagaacg
     1201 gctacgacaa gcagcccgag aaaaccatgt tcatcatcga gcggctgcag agctacttcc
     1261 cattcctgaa gatcatggcc gagaaccaga gagagtacag caacggcaag tacaagcaga
     1321 accgcgtgga agtgaacagc aacgacatct tcgaggtgct gaagcgcgcc ttcggcgtgc
     1381 tgaagatgta cagggacctg accaacgcat acaagaccta cgaggaaaag ctgaacgacg
     1441 gctgcgagtt cctgaccagc acagagcaac ctctgagcgg catgatcaac aactactaca
     1501 cagtggccct gcggaacatg aacgagagat acggctacaa gacagaggac ctggccttca
     1561 tccaggacaa gcggttcaag ttcgtgaagg acgcctacgg caagaaaaag tcccaagtga
     1621 ataccggatt cttcctgagc ctgcaggact acaacggcga cacacagaag aagctgcacc
     1681 tgagcggagt gggaatcgcc ctgctgatct gcctgttcct ggacaagcag tacatcaaca
     1741 tctttctgag caggctgccc atcttctcca gctacaatgc ccagagcgag gaacggcgga
     1801 tcatcatcag atccttcggt atcaacagca tcaagctgcc caaggaccgg atccacagcg
     1861 agaagtccaa caagagcgtg gccatggata tgctcaacga agtgaagcgg tgccccgacg
     1921 agctgttcac aacactgtct gccgagaagc agtcccggtt cagaatcatc agcgacgacc
     1981 acaatgaagt gctgatgaag cggagcagcg acagattcgt gcctctgctg ctgcagtata
     2041 tcgattacgg caagctgttc gaccacatca ggttccacgt gaacatgggc aagctgagat
     2101 acctgctgaa ggccgacaag acctgcatcg acggccagac cagagtcaga gtgatcgagc
     2161 agcccctgaa cggcttcggc agactggaag aggccgagac aatgcggaag caagagaacg
     2221 gcaccttcgg caacagcggc atccggatca gagacttcga gaacatgaag cgggacgacg
     2281 ccaatcctgc caactatccc tacatcgtgg acacctacac acactacatc ctggaaaaca
     2341 acaaggtcga gatgtttatc aacgacaaag aggacagcgc cccactgctg cccgtgatcg
     2401 aggatgatag atacgtggtc aagacaatcc ccagctgccg gatgagcacc ctggaaattc
     2461 cagccatggc cttccacatg tttctgttcg gcagcaagaa aaccgagaag ctgatcgtgg
     2521 acgtgcacaa ccggtacaag agactgttcc aggccatgca gaaagaagaa gtgaccgccg
     2581 agaatatcgc cagcttcgga atcgccgaga gcgacctgcc tcagaagatc ctggatctga
     2641 tcagcggcaa tgcccacggc aaggatgtgg acgccttcat cagactgacc gtggacgaca
     2701 tgctgaccga caccgagcgg agaatcaaga gattcaagga cgaccggaag tccattcgga
     2761 gcgccgacaa caagatggga aagagaggct tcaagcagat ctccacaggc aagctggccg
     2821 acttcctggc caaggacatc gtgctgtttc agcccagcgt gaacgatggc gagaacaaga
     2881 tcaccggcct gaactaccgg atcatgcaga gcgccattgc cgtgtacgat agcggcgacg
     2941 attacgaggc caagcagcag ttcaagctga tgttcgagaa ggcccggctg atcggcaagg
     3001 gcacaacaga gcctcatcca tttctgtaca aggtgttcgc ccgcagcatc cccgccaatg
     3061 ccgtcgagtt ctacgagcgc tacctgatcg agcggaagtt ctacctgacc ggcctgtcca
     3121 acgagatcaa gaaaggcaac agagtggatg tgcccttcat ccggcgggac cagaacaagt
     3181 ggaaaacacc cgccatgaag accctgggca gaatctacag cgaggatctg cccgtggaac
     3241 tgcccagaca gatgttcgac aatgagatca agtcccacct gaagtccctg ccacagatgg
     3301 aaggcatcga cttcaacaat gccaacgtga cctatctgat cgccgagtac atgaagagag
     3361 tgctggacga cgacttccag accttctacc agtggaaccg caactaccgg tacatggaca
     3421 tgcttaaggg cgagtacgac agaaagggct ccctgcagca ctgcttcacc agcgtggaag
     3481 agagagaagg cctctggaaa gagcgggcct ccagaacaga gcggtacaga aagcaggcca
     3541 gcaacaagat ccgcagcaac cggcagatga gaaacgccag cagcgaagag atcgagacaa
     3601 tcctggataa gcggctgagc aacagccgga acgagtacca gaaaagcgag aaagtgatcc
     3661 ggcgctacag agtgcaggat gccctgctgt ttctgctggc caaaaagacc ctgaccgaac
     3721 tggccgattt cgacggcgag aggttcaaac tgaaagaaat catgcccgac gccgagaagg
     3781 gaatcctgag cgagatcatg cccatgagct tcaccttcga gaaaggcggc aagaagtaca
     3841 ccatcaccag cgagggcatg aagctgaaga actacggcga cttctttgtg ctggctagcg
     3901 acaagaggat cggcaacctg ctggaactcg tgggcagcga catcgtgtcc aaagaggatg
     3961 gcggtagtgg aggaagcggg gggagcggag ggagtggtgg atccggcggc tccatgcacg
     4021 atgctgtttt tgggcagcct gggggcctgg ggaacaacat ctatcagcac aggttcaatt
     4081 ttttccctga aaaccctgcg ttctcagcat gggggacaag tgggtctcaa ggtcagcaga
     4141 cccagagctc cgcgtatggg agcagctaca cctacccccc gagctccctg ggtggcacgg
     4201 tggttgatgg gcagccaggc tttcacagcg acaccctcag caaggccccc gggatgaaca
     4261 gcctggagca gggcatggtt ggcctgaaga ttggggacgt cagctcctcc gccgtcggat
     4321 ccgattacaa ggacgacgat gacaagtagt gtttaaacga attcgggctc ggtacacggg
     4381 gatcctctag agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa
     4441 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc
     4501 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4561 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4621 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4681 gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat
     4741 tctaatggat cctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     4801 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     4861 agcccttgcg ccctgagtgc ttgcggcagc gtgagcttca aaagaattgc cagctggggc
     4921 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     4981 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgacggt cgtttcgcat
     5041 gcttgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5101 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5161 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5221 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5281 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5341 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5401 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5461 cgagcgagca cgcactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5521 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgta tgccggatgg
     5581 tgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5641 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5701 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5761 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5821 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaaccgg
     5881 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5941 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6001 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6061 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6121 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6181 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6241 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6301 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6361 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6421 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6481 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6541 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt caagaagatc
     6601 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6661 tggtcatgag attatcaaaa aggatcttca cctagatcct tttatagtcc ggaaatacag
     6721 gaacgcacgc tggatggccc ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag
     6781 tggattaagt gggggtaatg tggcctgtac cctctggttg cataggtatt catacggtta
     6841 aaatttatca ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt tttacctgtc
     6901 tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta caattaaggg attatggtaa
     6961 atccacttac tgtctgccct cgtagccatc gagataaacc gcagtactcc ggccacgatg
     7021 cgtccggcgt agaggatcga gatcttcaat attggccatt agccatatta ttcattggtt
     7081 atatag
//
