LOCUS       sequence-231788-        5368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse GFR alpha 1 In situ hybridization probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD
  TITLE     Molecular identification of rapidly adapting mechanoreceptors and 
            their developmental dependence on ret signaling.
  JOURNAL   Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003.
  PUBMED    20064391
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.11"; journalName: "Neuron"; date: 
            "2009-12-24- 24"; volume: "64"; issue: "6"; pages: "841-56"
COMMENT     Sequence Label: pCRII-TOPO GFR alpha 1
FEATURES             Location/Qualifiers
     source          1..5368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1801..1820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1802..1820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1827..1844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1827..1843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1836..1858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1984..2412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2071..2090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2281..2302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             2756..3550
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2810..2829)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3420..3439
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(3568..3636,3637..4428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3786..3805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4599..5187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5088..5107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5341..5358
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttctac gatgtttctg ccaatgatac
      361 agacaacagg gcagccagag cggtgaacac catcaccggc agtgagctca gaccgcagct
      421 gggaggagca gccattgatt ttgtggttat gtggctggag gcaccagcga gaccatcctt
      481 tccgtaatca ttatcagaaa gacagagatg tatactgccc gatacattgg atttcagctt
      541 ctgtgcctgc aaattagcac acggtggtaa aacgtgtgtg ggaatctcat tctcagagcc
      601 tgctggccct agaggcttgt tcttgatccg gaaggcagtg gtagtcgtgg cagtggtggt
      661 ctggactggg ggggctggct gccacatggt cacatccgag ccattgccaa aggcttgaat
      721 tgcatttttg agacacgtat tgtccttaaa aaaattcaga aacttcaggc aatcttccag
      781 gtcattgcca ctgttgctgc aatcgcacca cggcgccaca ctgaggctgc tggagtctat
      841 gtagttagga gtcatgactg tgccaatcag tcccgagtag gccaggaggc agtctgcgta
      901 gttctcctta agacagttgc tgacagacct tgactctggc tggcagttgg taaaaaaatc
      961 tgcaaggcga gatctgcaga tgtaatttgt cttgcaggag tcttgcagat tcaggcagtt
     1021 gggcctctct cgttcttcat aggagcacac agggacgata gtctgtcgcc gcctctcggt
     1081 gcaggcgacg tcccggcagg agcagaagag catcccgtag ctgtgcttgg ctggaacttt
     1141 gtcgaagaac tgcctgaggg ctttgtggca cttgcggcgg ttgcagactt cattggacat
     1201 gctggtggta cagggggtga tgtaggcgga cctgtacttc ttgcaggtgt catccaggtt
     1261 gcaggccttg gctgcatcga ggcagttgtt ccctttggaa atgtgttcca ctgatatgaa
     1321 cgggactgcc cggaatatat ctgacagcct gctgttaacc ggctcatatg gggaatcttc
     1381 cagtaggtca cttccctgca ggctctggta catgctccag tagatacgca gacaattctt
     1441 ctctttcttc atgccccgct tgcagcggca gttgtagaga gacttctgct tcaaggcctc
     1501 catagcgctg cggcactcat ccttggcctc gaggccggat gtcaggctga agttggtttc
     1561 cttgcccgcc acgcactgcc tcagtgtgcg gtacttggtg ctgcagctct gttccttcag
     1621 gcactgatca ctggctttca cacagtccag gcggtcccca ccactcacct cggccgacat
     1681 cagcaaatcc aggagtggca gcacgaagta cagagtggct aggaacatgg taagggcgaa
     1741 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg
     1801 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     1861 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1981 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2041 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2161 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2221 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2281 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2341 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2401 ttaacgcgaa ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta
     2461 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     2521 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     2581 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     2641 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     2701 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     2761 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     2821 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     2881 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     2941 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3001 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3061 cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3121 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3181 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3241 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     3301 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3361 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3421 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3481 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3541 gttcttctga attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3601 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3661 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3721 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3781 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3841 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3901 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3961 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4021 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4081 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4141 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4201 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4261 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4321 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4381 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4441 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4501 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4561 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4621 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4681 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4741 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4801 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4861 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4921 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4981 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5041 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5101 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5161 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5221 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5281 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5341 agcgagtcag tgagcgagga agcggaag
//
