LOCUS       sequence-231804-        7525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a 
            BB0323-mCherry translational fusion under the control of the bb0826 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2G_P0826-BB0323-mCherryBb
FEATURES             Location/Qualifiers
     source          1..7525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    673..694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(709..714,715..732,733..739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    747..763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     752..774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     771..787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     771..787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2914..2931)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2914..2930)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2923..2945)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3497..4030
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      4133..4721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4622..4641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gataagtttt ccatttatag ctccttgaat accggatttt ttatctataa agtatatata
       61 tcacaatttt gatttaaaag taaattcttt ttttaaaaat acaaaaattt tttataaatt
      121 atatagacca tgatattgta ttaatactaa ttgattttaa tttaaggttg aactaaaata
      181 aacatagttt tgtagaaaat aatttttcat tattttgcta cttgatattg gatcgtaaaa
      241 atattagggc tttacaaagt tcttttaaaa gagaatttag taaagcccta agtcttttaa
      301 taaaaaattt tttgtaaaaa agttggcaaa aatagttttt gctatatatt tatatataag
      361 aaaaaattca atactcaagg agataaaaaa tggaaaactt aaacaataat ccacaaaaaa
      421 ttaatcaaat aaataagcaa actaatttta ttggttttga aaattttata gaacttgata
      481 atcaagttaa tcaaactaaa gaaaaaagta aattaagtaa gataggtaag aaattacccg
      541 gaataagcag tcaagtctag tgcggccgcg cccaatacgc aaaccgcctc tccccgcgcg
      601 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      661 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      721 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      781 ctatgacatg attacgaatt cgagctcggc aatagaagaa tctatagaaa gcataaaaag
      841 ccttatagaa aacaaagaat acatacaaaa gttacatttt ataaaaaaat taataaataa
      901 ggtttacaaa aaattaaatt acttttttta aaaactaaac tttgaaagcc ttgttataat
      961 ataaaatata ataatcaaat attattcaaa gttaacagca atgaagttta taataaattg
     1021 gatccaggag gtctatgaat ataaagaata aattaatatc gctgctaatt gtagtagcaa
     1081 tctcttttat cgcatgcaaa acgcctccag aatcaagaga gagtaaaaat gctaaaattg
     1141 cacaaccaga taataaaaat tttcaattaa gagatataaa agacattaaa aatgaactaa
     1201 taagagaacg gggacatctt ttttattcca aagagtttaa tgaagctgaa agattagaag
     1261 aagcaatgaa acaaagtttt tccaaaaaaa aagcaataga aggaaatgaa attgctctaa
     1321 aagtacttga aagatacaaa acaataataa gagaaacgag ggaaaaaaaa gaaaaaacaa
     1381 attaccttaa agaaaatatt gaaaagtatc taaacgatgc agaagcaaat gaagcataca
     1441 tatggatccc gctggaaatc gacgaagtaa ataatttata ttttgaagca acaagaaaat
     1501 ataaaaatta cgatcttgat aatgcccttg acatgtatag caaggccttt aacagagcac
     1561 aacaagcagc aaaaaatgcc aaagaagcta aagctttaaa agaaactgat gagagaatgt
     1621 acaaacaatt aaaagcactt gaagcggctt ccaacttacc aatttatagc aataataagc
     1681 ttataaagcc atctccatgg aatggtagag cattcattaa agaaagaaac agtcacttaa
     1741 accttttaaa tactaataaa gacacttatc ttcttggaga agcagaaatt tcaattccaa
     1801 tagtactcgc atacgaagaa aaagtggaaa tagcaaaaaa ctccaaacct caagaacaat
     1861 tcaaaacgct agaacttatt gaaagatcta gaacattgtg ggaaaaaggg gttgaggcta
     1921 aaaatgtaaa aaactttaga cttgccaatg aattattttt agaatctgca agataccttg
     1981 aagcttatca aagcaatgca agcagcgagc tttacgtaat aaaaataggc aataccctat
     2041 ggggcatttc caaaaaatta tacaacgatc cttacttatg gccaaaaatt tggtttgcca
     2101 acagacaaaa aatacaaaat ccggatctaa ttcattctaa ctggaagata ataattcctg
     2161 ccaaagggta ccgatcctct agagtcgacc tgcagatggt tagtaaaggt gaagaagata
     2221 atatggctat tattaaagaa tttatgagat ttaaagttca catggaaggt agtgttaatg
     2281 gtcatgaatt tgaaattgaa ggtgaaggtg aaggtagacc ttatgaaggt actcaaactg
     2341 ctaaattaaa agttactaaa ggtggtcctt taccttttgc ttgggatatt ttaagtcctc
     2401 aatttatgta tggtagtaaa gcatacgtta aacatcctgc tgatattcct gattatttaa
     2461 aattaagttt tcctgaaggt tttaaatggg aaagagttat gaattttgaa gatggtggtg
     2521 ttgttactgt tactcaagat agtagtttac aagatggtga atttatttat aaagttaaat
     2581 taagaggtac taattttcct agtgatggtc ctgttatgca aaaaaaaact atgggttggg
     2641 aagctagtag tgaaagaatg tatcctgaag atggtgcttt aaaaggtgaa attaaacaaa
     2701 gattaaaatt aaaagatggt ggtcattatg atgctgaagt taaaactact tataaagcta
     2761 aaaaacctgt tcaattacct ggtgcttata atgttaatat taaattagat attacttcgc
     2821 ataatgaaga ttatactatt gttgaacaat atgaaagagc tgaaggtaga catagtacag
     2881 gtggtatgga tgaattatat aaataagctt ggcactggcc gtcgttttac aacgtcgtga
     2941 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3001 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3061 tggcgaatgg cgcggccgcc ctaggttaat acccgagctt caaggaagat ttcctattaa
     3121 ggttgaactt aagagcttaa gcatagacga tttgaaaaaa attttaaaac aaacaaaaaa
     3181 ttctttaata aaacaatatg ttgcgatgtt taaggtttat gatttagatt taaagtttag
     3241 tgaggaagct atagatagaa ttgcagagct tacttttaat atgaatcttg agagtgaaaa
     3301 tcttggtgcc agaagacttc acggtgttat ggaaatagtg cttgcagatc ttttttttga
     3361 agtgcctggc agtaagttga aaaaatttga aataaacttg gactatgtta ataaaaaaat
     3421 acaaattaac gaacaaaaag atttgaatta ttatataatt tagttaaaag caattttaaa
     3481 tgagggaggt ttccatatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
     3541 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
     3601 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
     3661 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
     3721 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
     3781 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
     3841 cgagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
     3901 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
     3961 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     4021 cgccacctaa caattcgttc aagccgagat cggcttacgc gtccaaaatc ccttaacgtg
     4081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4261 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4501 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4681 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4741 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcactagatc
     4801 cccggcaagt tcatccttag ggttttcata tctcttaaaa agtcaatttc tgcttgatta
     4861 atttcttgtg gattattgtt tttgcggttt ttcatttttt atctccttga gtattgaatt
     4921 ttttcttata tataaaatat agcaaaaact atttttgcca acttttttac aaaaaatttt
     4981 ttattaaaac acttagggct ttgctaaatt ctcttttaaa agaacttagt aaagccctat
     5041 ggatttaaga actgattata cttacgtagt aaaaatacta cagatattaa tagtatagac
     5101 catatcaaaa tcataatcaa tttatattta tctaaaattg tggtccatac aaaatacaaa
     5161 aaaattaggt ttttctaaat tctattttaa aagaacttag taacacctaa tctctaaatt
     5221 acttacaaaa ttctaaaatt agatttttag ttcttcatat ttctttaaaa gttttttaag
     5281 aaaatctttt tgattttcaa aaatttcatg catcataaaa ctagtaaatt tggatttttt
     5341 tttgtaaaaa tcataacttt cttgggtttt aagttgaaat cttaatggtt ttattggatt
     5401 ttgcctagat ttttttaacg ccggactttc tttatctttt ataaaattat aagaattttt
     5461 aataccattt tctattaaat aatcttcttt tataagcccc tcttgcagtg cagttgctaa
     5521 tcttaaatac ttatacgctt gagtttttgc tacatcaaag tcttttataa aatcaagaaa
     5581 agttttataa ccatcaaatt tataatattg attatctttt atttctttta aaattttaat
     5641 attttccacc ctgtggaata tttctttttt gaaattgtca tttaactttt cctttagtgt
     5701 cttgtaataa gaaattttac tttcattaat cattaaattt tgttcaacat aagtatctat
     5761 cttttttgtt aaaactttaa caacacgttt tttttccata aaaattttcc tttatttttg
     5821 caacattttc cttaattgat ataattgtta ctattttaga ttttttaata tccacttaag
     5881 aattaatccc tcactagaat attttttatt atagaattca tatacatgtt tttctaaatc
     5941 tataaattta tcaacaattg ctttattata gggtgtatct atcttttctt ttcttaataa
     6001 tcgtctcata ttctttaaat aacaacaaac actgccggtt ttaaacctaa attctatgta
     6061 atatattttt gaaaaagtat atacattttc aaccccgttt atttcatact ttacaaaaac
     6121 attatttatt ggttttctat aaccatagta aatgcctaaa aatttgtccc cgtctttcat
     6181 aggatataga taaagttttt gaatctctga tttatctaat aatttttcaa aaaaaattat
     6241 acaccgcttt tgtttgttac taactactct atgcaatgcc tttaatattt ttgtgtgata
     6301 tatattttta tcaccttttt tttctttcag caggaagata ctttcttttg gaaatttaat
     6361 tttttgttca tttattcttg cagccttaag tccgattttc atatttaatc cttatatagc
     6421 ctttctattt aattctttta aactcaataa acttttttgg tttttcatta gttctaataa
     6481 ttcgcaataa tatgtgcaaa acactttatt gtattctatt ttcttttggt tattcaaata
     6541 ttcttttata attggcttta gaagttcaat atttacttct ttttttagtt gttcaataag
     6601 gatattgaaa atgtttgttt ttaaatcttg ataattttgt tgagaatttt ctttttttct
     6661 ttcaattgac ttctctaatt tacgctttat attgtttaaa tcgctatatt tatgactttc
     6721 aataataaaa tgaggcttaa atttgtaatt ttcgtatatt ttttggaaac ttgtttctaa
     6781 ttgtttagaa ttatattcat ttttttcaaa ttctttttga gtgttgcata gaatttcctt
     6841 taatttttct tgtttttctt tcatacaaga tttagtaaaa tgtatatttt tattttttag
     6901 taatttaatt tctattattt ttatgatttt aagtattttg attatattat ctttatcaat
     6961 atttaaattg aataaaattg taagaaaaat tgaaagaatt tttttacata aaaagttaca
     7021 tttattgaag taatttctta cttgatactt ttctatttca ttaatctttc tttcttcttt
     7081 tatattattt ttattattta aacactcctt taaatctaca ctcccatttt tagtaaatct
     7141 atctttaagg ccccttgaaa ctctattttg aaatcttaaa tctttttttt cttgaaagta
     7201 tttatagatt ttttggtaac attctttttt tgaaaaatta agttgatagt atatttcggt
     7261 ccccaaattt acccctaagt gtttgtgata attagttgtg actttaaatt ctttttctaa
     7321 tttgtaaagg taattttgca gtgtttttag tttaacgggt gtttgactat ttcttttaag
     7381 gttttcatta aagtaataga gtatgttttt ttgtgtgtat ttcttatatg ttttatttat
     7441 gtacgctagt gttgagataa ggactactaa tttatactgt aatttattgt agcaagttgt
     7501 ggttttactg gttttgttta tatta
//
