LOCUS       sequence-231814-        4597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BLINK2 gene in pDONR vector for Gateway cloning system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Alberio L, Locarno A, Saponaro A, Romano E, Bercier V, Albadri S, 
            Simeoni F, Moleri S, Pelucchi S, Porro A, Marcello E, Barsotti N, 
            Kukovetz K, Boender AJ, Contestabile A, Luo S, Moutal A, Ji Y, 
            Romani G, Beltrame M, Del Bene F, Di Luca M, Khanna R, Colecraft HM,
            Pasqualetti M, Thiel G, Tonini R, Moroni A
  TITLE     A light-gated potassium channel for sustained neuronal inhibition.
  JOURNAL   Nat Methods. 2018 Nov;15(11):969-976. doi: 
            10.1038/s41592-018-0186-9. Epub 2018 Oct 30.
  PUBMED    30377377
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0186-9"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "969-976"
COMMENT     Sequence Label: pDONR-BLINK2
FEATURES             Location/Qualifiers
     source          1..4597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1328..1427)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1539..1558)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1617..1636)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2210..2743)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2932..2937,2938..2954,2955..2960))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3617..4205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4106..4125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4340..4367)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4459..4545)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct tcaccatggg atgtacagtc
      121 tctgcagagt tggctactac acttgaacgt attgagaaga actttgtcat tactgaccca
      181 agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt gacagaatat
      241 agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga aactgatcgc
      301 gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac tgttcagctg
      361 attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca gcctatgcga
      421 gatcagaagg gagatgtcca gtactttatt ggggttgatt tggatggaac tgagcatgtc
      481 cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga agtgtttagt
      541 aaatttctaa cgcgaactga accattcatg atacatctct ttattctcgc aatgttcgtg
      601 atgatctata aattcttccc gggagggttc gaaaataact tctctgttgc aaacccggac
      661 aaaaaggcat catggataga ttgtatatac ttcggagtaa cgacacactc tactgtcgga
      721 ttcggagata tactgccaaa gacgaccggc gcaaagcttt gtacgatagc acatatagta
      781 acagtgttct tcatcgttct aactttagat gattcgaata ctagtggtca cgaaaacaga
      841 gatttcaaaa gcatgggatg ggaagagtgg agagattcaa gaaaagatgg ctatggttta
      901 gatgttacga atccgacttc cgacactgct ctaatggatg cgattcacaa ggaagatact
      961 gaaatggtta agaagatact taaggaacaa aagatagaga gagccaaagt ggaaagatca
     1021 agtagtgaaa ccgctggaag aagttacgct aacgattcat cgaaaaaaga tccatattgc
     1081 agctcaagca accaaatcat caagccatgc aaacgagaag aaaagagagt taccatccac
     1141 atgatgtctg agagcaagaa cgggaagttg atactcttac catcatccat agaagagctt
     1201 ctaagacttg caagtgagaa gtttggaggc tgcaacttca caaagatcac caatgcggac
     1261 aacgctgaga ttgatgattt agatgtcatt tgggatggtg atcatttgta tttttcagat
     1321 aattgagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1381 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
     1441 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1501 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     1561 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     1621 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     1681 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1741 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1801 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     1861 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     1921 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     1981 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2041 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     2101 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     2161 tgacgagggg aaattaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     2221 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     2281 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     2341 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     2401 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     2461 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     2521 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     2581 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     2641 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     2701 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     2761 atcaaacatc gacccacggc gtaaagcgct tgctgcttgg atgcccgagg catagactgt
     2821 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2881 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     2941 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     3001 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     3061 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     3121 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     3181 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     3241 catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctact aataggttgt
     3301 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3361 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3421 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3481 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3541 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3661 aaaaaccacc gctaccagcg gtggtttttt tgccggatca agagctacca actctttttc
     3721 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3781 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3841 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3901 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3961 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4021 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4081 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4141 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4201 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4261 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gctagccagg
     4321 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4381 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4441 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4501 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     4561 ctactctcgc gttaacgcta gcatggatct cgggccc
//
