LOCUS       sequence-231815-        5959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualize localization of ER enzyme CEPT1, which converts 
            diacylglycerol precursors to phosphatidylethanolamine or 
            phosphatidylcholine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   English AR, Voeltz GK
  TITLE     Rab10 GTPase regulates ER dynamics and morphology.
  JOURNAL   Nat Cell Biol. 2013 Feb;15(2):169-78. doi: 10.1038/ncb2647. Epub 
            2012 Dec 23.
  PUBMED    23263280
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2647"; 
            journalName: "Nat Cell Biol"; date: "2013-02"; volume: "15"; issue: 
            "2"; pages: "169-78"
COMMENT     Sequence Label: mCherry-CEPT1
FEATURES             Location/Qualifiers
     source          1..5959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      600..609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             606..1313
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(751..769)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1019..1038
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2740..2861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2777..2796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2831..2850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2868..3323)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3005..3026)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3217..3236
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3350..3454
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3456..3813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3456..3647
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3479..3499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3664..3799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3848..4642
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3848..4642
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3902..3921)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4512..4531
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4830..4849)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4874..4921
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5250..5838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5739..5758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
      601 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      661 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      721 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      781 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      841 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      901 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      961 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1021 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1081 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     1141 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1201 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1261 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtccggac
     1321 tcagatctat gagtgggcat cgatcaacaa ggaaaagatg tggagattct cacccggagt
     1381 ccccagtggg cttcgggcat atgagtacta caggatgtgt attaaataaa ttgtttcagt
     1441 taccaacacc accattgtca agacaccaac taaagcggct agaagaacac agatatcaaa
     1501 gtgctggacg gtccctgctt gagcccttaa tgcaagggta ttgggaatgg ctcgttagaa
     1561 gagttccctc ctggattgcc ccaaatctca tcaccatcat tggactgtca ataaacatct
     1621 gtacaactat tttattagtc ttctactgcc ctacagctac agagcaggca cctctgtggg
     1681 catatattgc ttgtgcctgt ggccttttca tttaccagtc tttggatgct attgatggga
     1741 aacaggcaag aagaaccaat agtagttctc ctctgggaga actttttgat catggctgtg
     1801 attcactatc aacagttttt gtggttcttg gaacttgtat tgcagtgcag ctggggacaa
     1861 accctgattg gatgtttttt tgttgttttg cggggacatt tatgttctat tgtgcgcact
     1921 ggcaaacgta tgtttctgga acattgcgat ttggaataat tgatgtgact gaagtgcaaa
     1981 tcttcataat aatcatgcat ttgctggcag tgattggagg accacctttt tggcaatcta
     2041 tgattccagt gctgaatatt caaatgaaaa tttttcctgc actttgtact gtagcaggga
     2101 ccatattttc ctgtacaaat tacttccgtg taatcttcac aggtggtgtt ggcaaaaatg
     2161 gatcaacaat agcaggaaca agtgtccttt ctccttttct ccatattgga tcagtgatta
     2221 cattagctgc aatgatctac aagaaatctg cagttcagct ttttgaaaag catccctgtc
     2281 tttatatact gacatttggt tttgtgtctg ctaaaatcac taataagctt gtggttgcac
     2341 acatgacgaa aagtgaaatg catttgcatg acacagcatt cataggtccg gcacttttgt
     2401 ttctggacca gtattttaac agctttattg atgaatatat tgtactttgg attgccctgg
     2461 ttttctcttt ctttgatttg atccgctact gtgtcagtgt ttgcaatcag attgcgtctc
     2521 acctgcacat acatgtcttc agaatcaagg tctctacagc tcattctaat catcattaag
     2581 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
     2641 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2701 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2821 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     2881 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2941 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3001 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3061 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3121 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3181 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3241 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3301 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3421 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3481 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3781 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3841 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3901 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3961 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4021 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4081 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4141 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4201 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4261 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4321 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4381 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4441 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4501 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4561 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4621 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4681 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4741 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4801 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4861 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     4921 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4981 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5041 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5101 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5161 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5221 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5281 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5341 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5401 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5461 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5521 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5581 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5641 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5701 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5761 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5821 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5881 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5941 accgccatgc attagttat
//
