LOCUS       sequence-231819-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, 
            Savage DF, Liu DR
  TITLE     Circularly permuted and PAM-modified Cas9 variants broaden the 
            targeting scope of base editors.
  JOURNAL   Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y.
  PUBMED    31110355
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y."
COMMENT     Sequence Label: VRQR-ABEmax
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        520..723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     674..694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     765..784
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        765..783
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             833..853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2042..6142
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6185..6205
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6214..6231
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6254..6271)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6260..6484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6555..6571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6555..6571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6568..6590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6579..6595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6603..6609,6610..6627,6628..6633))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6648..6669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6786..6803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6957..7545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7037..7056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7716..8507,8508..8576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8339..8358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8577..8681)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8760..8779)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
       61 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      721 gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac gactcactat
      781 agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt caccaaagaa
      841 gaagcggaaa gtctctgaag tcgagtttag ccacgagtat tggatgaggc acgcactgac
      901 cctggcaaag cgagcatggg atgaaagaga agtccccgtg ggcgccgtgc tggtgcacaa
      961 caatagagtg atcggagagg gatggaacag gccaatcggc cgccacgacc ctaccgcaca
     1021 cgcagagatc atggcactga ggcagggagg cctggtcatg cagaattacc gcctgatcga
     1081 tgccaccctg tatgtgacac tggagccatg cgtgatgtgc gcaggagcaa tgatccacag
     1141 caggatcgga agagtggtgt tcggagcacg ggacgccaag accggcgcag caggctccct
     1201 gatggatgtg ctgcaccacc ccggcatgaa ccaccgggtg gagatcacag agggaatcct
     1261 ggcagacgag tgcgccgccc tgctgagcga tttctttaga atgcggagac aggagatcaa
     1321 ggcccagaag aaggcacaga gctccaccga ctctggagga tctagcggag gatcctctgg
     1381 aagcgagaca ccaggcacaa gcgagtccgc cacaccagag agctccggcg gctcctccgg
     1441 aggatcctct gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc
     1501 caagagggca cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag
     1561 agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga
     1621 aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac
     1681 cctgtacgtg acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat
     1741 cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga
     1801 cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga
     1861 tgaatgtgcc gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca
     1921 gaagaaggcc cagagctcca ccgactccgg aggatctagc ggaggctcct ctggctctga
     1981 gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc
     2041 agacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
     2101 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     2161 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     2221 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     2281 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     2341 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     2401 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     2461 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     2521 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     2581 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     2641 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     2701 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     2761 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     2821 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     2881 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     2941 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     3001 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     3061 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     3121 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     3181 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     3241 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     3301 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     3361 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     3421 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     3481 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     3541 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     3601 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     3661 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     3721 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     3781 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     3841 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     3901 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     3961 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     4021 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     4081 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     4141 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     4201 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     4261 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     4321 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     4381 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     4441 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     4501 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     4561 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     4621 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     4681 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     4741 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     4801 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     4861 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     4921 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     4981 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     5041 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     5101 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     5161 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     5221 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     5281 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     5341 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     5401 cagaaagaag gactgggacc ctaagaagta cggcggcttc gtgagcccca ccgtggccta
     5461 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     5521 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     5581 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     5641 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctcagcca gagaactgca
     5701 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     5761 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     5821 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     5881 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     5941 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     6001 tgccgccttc aagtactttg acaccaccat cgaccggaag cagtacagaa gcaccaaaga
     6061 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     6121 cctgtctcag ctgggaggtg actctggcgg ctcaaaaaga accgccgacg gcagcgaatt
     6181 cgagcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa
     6241 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6301 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6361 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6421 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6481 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     6541 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6601 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     6661 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6721 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6781 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6841 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6901 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6961 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7021 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7081 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7141 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7201 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7261 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7321 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7381 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7441 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7501 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7561 ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7621 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7681 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7741 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7801 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7861 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7921 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7981 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8041 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8101 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8161 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8221 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8281 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8341 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8401 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8461 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8521 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8581 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8641 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8701 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt
     8761 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg t
//
