LOCUS       sequence-23195-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Jen J, Harper JW, Bigner SH, Bigner DD, Papadopoulos N, Markowitz S,
            Willson JK, Kinzler KW, Vogelstein B
  TITLE     Deletion of p16 and p15 genes in brain tumors.
  JOURNAL   Cancer Res. 1994 Dec 15. 54(24):6353-8.
  PUBMED    7987828
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            1994 Dec 15. 54(24):6353-8."
COMMENT     Sequence Label: pCRII p15
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(646..664)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(647..664)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(682..698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(682..698)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(695..717)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    706..722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctcgagcggc cgccagtgtg atggatatct gcagaattcg gcttcatgcc atggctatgc
       61 gcgaggagaa caagggcatg cccagtgggg gcggcagcga tgagggtctg gccagcgccg
      121 cggcgcgggg actagtggag aaggtgcgac agctcctgga agccggcgcg gatcccaacg
      181 gagtcaaccg tttcgggagg cgcgcgatcc aggtcatgat gatgggcagc gcccgcgtgg
      241 cggagctgct gctgctccac ggcgcggagc ccaactgcgc agaccctgcc actctcaccc
      301 gaccggtgca tgatgctgcc cgggagggct tcctggacac gctggtggtg ctgcaccggg
      361 ccggggcgcg gctggacgtg cgcgatgcct ggggtcgtct gcccgtggac ttggccgagg
      421 agcggggcca ccgcgacgtt gcagggtacc tgcgcacagc cacaggggac tgacgcctgg
      481 ttccccagcc gcccacaacg actttatttt cttacccaat ttcccacccc cacccaccta
      541 attcgatgaa ggctgccaac ttaaggcaag ccgaattcca gcacactggc ggccgttact
      601 agtggatccg agctcggtac caagcttgat gcatagcttg agtattctat agtgtcacct
      661 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      721 aattccacac aacatacgag ccggaagcat aaagt
//
