LOCUS       sequence-23196-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Jen J, Harper JW, Bigner SH, Bigner DD, Papadopoulos N, Markowitz S,
            Willson JK, Kinzler KW, Vogelstein B
  TITLE     Deletion of p16 and p15 genes in brain tumors.
  JOURNAL   Cancer Res. 1994 Dec 15. 54(24):6353-8.
  PUBMED    7987828
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            1994 Dec 15. 54(24):6353-8."
COMMENT     Sequence Label: pCRII p16
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(770..788)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(771..788)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(806..822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(806..822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(819..841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    830..846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(854..860,861..878,879..884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    899..920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctgctcgagc ggccgccagt gtgatggata tctgcagaat tcggcttggc cctgtaggac
       61 cttcggtgac tgatgatcta agtttcccga ggtttctcag agcctctctg gttctttcaa
      121 tcggggatgt ctgagggacc ttccgcggca tctatgcggg catggttact gcctctggtg
      181 ccccccgcag ccgcgcgcag gtaccgtgcg acatcgcgat ggccccgctc ctcagccagg
      241 tccacgggca gacggcccca ggcatcgcgc acgtccagcc gcgccccggc ccggtgcagc
      301 accaccagcg tgtccaggaa gccctcccgg gcagcgtcgt gcacgggtcg ggtgagagtg
      361 gcggggtcgg cgcagttggg ctccgcgccg tggagcagca gcagctccgc cactcgggcg
      421 ctgcccatca tcatgacctg gatcggcctc cgaccgtaac tattcggtgc gttgggcagc
      481 gcccccgcct ccagcagcgc ccgcacctcc tctacccgac cccgggccgc ggccgtggcc
      541 agctagtcag ccgaaggctc catgctgctc cccgccgccg gctccatgct gctccccgcc
      601 gcccgctgcc tgctctcccc ctctccgcag ccgccgagcg cacgcggtcc gccccaccct
      661 ctggtgacca gccagcccct cctctttctt caagccgaat tccagcacac tggcggccgt
      721 tactagtgga tccgagctcg gtaccaagct tgatgcatag cttgagtatt ctatagtgtc
      781 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      841 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      901 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      961 tgtcgtgcca gctgcatta
//
