LOCUS       sequence-232042-        8370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fusion protein used to visualise mitochondria upon the addition of 
            Halo specific dye, has hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   McArthur K, Whitehead LW, Heddleston JM, Li L, Padman BS, Oorschot 
            V, Geoghegan ND, Chappaz S, Davidson S, San Chin H, Lane RM, 
            Dramicanin M, Saunders TL, Sugiana C, Lessene R, Osellame LD, Chew 
            TL, Dewson G, Lazarou M, Ramm G, Lessene G, Ryan MT, Rogers KL, van 
            Delft MF, Kile BT
  TITLE     BAK/BAX macropores facilitate mitochondrial herniation and mtDNA 
            efflux during apoptosis.
  JOURNAL   Science. 2018 Feb 23;359(6378). pii: 359/6378/eaao6047. doi: 
            10.1126/science.aao6047.
  PUBMED    29472455
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-02-23"; volume: "359(6378). pii: 359/6378/eaao6047. 
            doi"; pages: " 10.1126/science.aao6047"
COMMENT     Sequence Label: pMIH-TOMM20-Halo
FEATURES             Location/Qualifiers
     source          1..8370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..342
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             409..825
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     755..777
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     793..809
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      838..847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1312..2202
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    2330..2792
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2386..2403)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2613..2632
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2831..3850
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             2831..3850
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             4185..4699
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4861..4877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4885..4891,4892..4909,4910..4915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4930..4951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5068..5085)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5239..5827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5319..5338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5998..6789,6790..6858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6621..6640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6859..6963)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7031..7049
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7087..7109)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7209..7228
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7422..7444
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7572..7591
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             7791..8307
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc
       61 taatgtttgc gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt
      121 ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt
      181 gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca
      241 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
      301 ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt
      361 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt
      421 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
      481 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
      541 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
      601 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
      661 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
      721 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
      781 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat tagatctgcc
      841 accatggtgg gtcggaacag cgccatcgcc gccggtgtat gcggggccct tttcattggg
      901 tactgcatct acttcgaccg caaaagacga agtgacccca acttcaagaa caggcttcga
      961 gaacgaagaa agaaacagaa gcttgccaag gagagagctg ggctttccaa gttacctgac
     1021 cttaaagatg ctgaagctgt tcagaagttc ttccttgaag aaatacagct tggtgaagag
     1081 ttactagctc aaggtgaata tgagaagggc gtagaccatc tgacaaatgc aattgctgtg
     1141 tgtggacagc cacagcagtt actgcaggtc ttacagcaaa ctcttccacc accagtgttc
     1201 cagatgcttc tgactaagct cccaacaatt agtcagagaa ttgtaagtgc tcagagcttg
     1261 gctgaagatg atgtggaagt cgacggtacc gcgggcccgg gatccaccgg tatggcagaa
     1321 atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac
     1381 tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc
     1441 tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct
     1501 ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac
     1561 cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc
     1621 attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc
     1681 aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa
     1741 tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc
     1801 gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa
     1861 gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg
     1921 cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa
     1981 gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca
     2041 ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag
     2101 gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc
     2161 agcgagatcg cgcgctggct gtcgacgctt gaaatttccg gctgactcga ggttaacgaa
     2221 ttccgccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     2281 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     2341 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     2401 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     2461 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     2521 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     2581 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     2641 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     2701 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     2761 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccgtac caaagatgga
     2821 tagatccgga aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     2881 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     2941 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     3001 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     3061 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     3121 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     3181 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     3241 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     3301 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     3361 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     3421 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     3481 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     3541 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     3601 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     3661 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     3721 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     3781 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     3841 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca
     3901 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt
     3961 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt
     4021 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt
     4081 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc
     4141 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     4201 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
     4261 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
     4321 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     4381 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     4441 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     4501 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     4561 agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     4621 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     4681 gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg
     4741 taggagtcga atattaagta atcctgactc aattagccac tgttttgaat ccacatactc
     4801 caatactcct gaaatagttc attatggaca gcgcagaaag agctggggag aattgtgaaa
     4861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5641 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     7021 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     7081 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     7141 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     7201 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     7261 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     7321 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     7381 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     7441 aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     7501 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     7561 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     7621 atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata
     7681 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
     7741 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     7801 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
     7861 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
     7921 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     7981 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     8041 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     8101 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     8161 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct
     8221 tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt
     8281 gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc
     8341 cagggaccac cgaccccccc gccgggaggt
//
