LOCUS       sequence-232051-       10103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for FOXM1c expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Barger CJ, Zhang W, Hillman J, Stablewski AB, Higgins MJ, 
            Vanderhyden BC, Odunsi K, Karpf AR
  TITLE     Genetic determinants of FOXM1 overexpression in epithelial ovarian 
            cancer and functional contribution to cell cycle progression.
  JOURNAL   Oncotarget. 2015 Jul 16.
  PUBMED    26243836
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2015 Jul 16."
COMMENT     Sequence Label: pCW57.1-FOXM1c
FEATURES             Location/Qualifiers
     source          1..10103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    139..160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(175..180,181..198,199..205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    213..229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     218..240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     237..253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     237..253
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     272..292
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        274..292
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        320..546
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             547..727
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    774..899
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1392..1625
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1810..1854
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2067..2184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2238..2552
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2246..2264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2282..2300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2317..2335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2353..2371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2389..2407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2424..2442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2460..2478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2525..2549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2573..2597
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2636..2645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2645..2668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             4001..4012
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(5002..5026)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5044..5061
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        5071..5581
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5500..5518
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5591..6187
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5591..5610)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6087..6107
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6188..6241
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6242..6859,6860..6865,6866..6901,6902..6904,6905..6940,
                     6941..6943,6944..6979,6980..6988)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7028..7048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7446..7457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7662..7895
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7967..8088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8004..8023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8058..8077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8128..8263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8263..8334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8335..8403,8404..9195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8553..8572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9366..9954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9855..9874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
       61 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      121 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      181 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      241 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
      301 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
      361 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      421 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      481 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      541 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      601 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      661 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      721 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      781 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      841 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      901 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      961 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1021 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1081 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1141 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1201 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1261 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1321 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1381 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1441 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1501 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1561 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1621 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1681 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1741 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1801 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1861 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1921 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1981 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacaatt ctcgacctcg
     2041 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2101 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2161 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     2221 ccgcgaatcg atatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2281 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
     2341 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     2401 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
     2461 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
     2521 gcagagctcg tttagtgaac cgtcagatcg cctggagaat tggctagcat caacaagttt
     2581 gtacaaaaaa gcaggctcgg gaattcgtcg actggatccg gtaccgagga gatctgccac
     2641 catggattac aaggatgacg acgataaggc gatcgccggc gcgccgaaaa ctagcccccg
     2701 tcggccactg attctcaaaa gacggaggct gccccttcct gttcaaaatg ccccaagtga
     2761 aacatcagag gaggaaccta agagatcccc tgcccaacag gagtctaatc aagcagaggc
     2821 ctccaaggaa gtggcagagt ccaactcttg caagtttcca gctgggatca agattattaa
     2881 ccaccccacc atgcccaaca cgcaagtagt ggccatcccc aacaatgcta atattcacag
     2941 catcatcaca gcactgactg ccaagggaaa agagagtggc agtagtgggc ccaacaaatt
     3001 catcctcatc agctgtgggg gagccccaac tcagcctcca ggactccggc ctcaaaccca
     3061 aaccagctat gatgccaaaa ggacagaagt gaccctggag accttgggac caaaacctgc
     3121 agctagggat gtgaatcttc ctagaccacc tggagccctt tgcgagcaga aacgggagac
     3181 ctgtgcagat ggtgaggcag caggctgcac tatcaacaat agcctatcca acatccagtg
     3241 gcttcgaaag atgagttctg atggactggg ctcccgcagc atcaagcaag agatggagga
     3301 aaaggagaat tgtcacctgg agcagcgaca ggttaaggtt gaggagcctt cgagaccatc
     3361 agcgtcctgg cagaactctg tgtctgagcg gccaccctac tcttacatgg ccatgataca
     3421 attcgccatc aacagcactg agaggaagcg catgactttg aaagacatct atacgtggat
     3481 tgaggaccac tttccctact ttaagcacat tgccaagcca ggctggaaga actccatccg
     3541 ccacaacctt tccctgcacg acatgtttgt ccgggagacg tctgccaatg gcaaggtctc
     3601 cttctggacc attcacccca gtgccaaccg ctacttgaca ttggaccagg tgtttaagcc
     3661 actggaccca gggtctccac aattgcccga gcacttggaa tcacagcaga aacgaccgaa
     3721 tccagagctc cgccggaaca tgaccatcaa aaccgaactc cccctgggcg cacggcggaa
     3781 gatgaagcca ctgctaccac gggtcagctc atacctggta cctatccagt tcccggtgaa
     3841 ccagtcactg gtgttgcagc cctcggtgaa ggtgccattg cccctggcgg cttccctcat
     3901 gagctcagag cttgcccgcc atagcaagcg agtccgcatt gcccccaagg tgctgctagc
     3961 tgaggagggg atagctcctc tttcttctgc aggaccaggg aaagaggaga aactcctgtt
     4021 tggagaaggg ttttctcctt tgcttccagt tcagactatc aaggaggaag aaatccagcc
     4081 tggggaggaa atgccacact tagcgagacc catcaaagtg gagagccctc ccttggaaga
     4141 gtggccctcc ccggccccat ctttcaaaga ggaatcatct cactcctggg aggattcgtc
     4201 ccaatctccc accccaagac ccaagaagtc ctacagtggg cttaggtccc caacccggtg
     4261 tgtctcggaa atgcttgtga ttcaacacag ggagaggagg gagaggagcc ggtctcggag
     4321 gaaacagcat ctactgcctc cctgtgtgga tgagccggag ctgctcttct cagaggggcc
     4381 cagtacttcc cgctgggccg cagagctccc gttcccagca gactcctctg accctgcctc
     4441 ccagctcagc tactcccagg aagtgggagg accttttaag acacccatta aggaaacgct
     4501 gcccatctcc tccaccccga gcaaatctgt cctccccaga acccctgaat cctggaggct
     4561 cacgccccca gccaaagtag ggggactgga tttcagccca gtacaaacct cccagggtgc
     4621 ctctgacccc ttgcctgacc ccctggggct gatggatctc agcaccactc ccttgcaaag
     4681 tgctcccccc cttgaatcac cgcaaaggct cctcagttca gaacccttag acctcatctc
     4741 cgtccccttt ggcaactctt ctccctcaga tatagacgtc cccaagccag gctccccgga
     4801 gccacaggtt tctggccttg cagccaatcg ttctctgaca gaaggcctgg tcctggacac
     4861 aatgaatgac agcctcagca agatcctgct ggacatcagc tttcctggcc tggacgagga
     4921 cccactgggc cctgacaaca tcaactggtc ccagtttatt cctgagctac agtagagccc
     4981 ctcgaggttt aaacggccgg cacccagctt tcttgtacaa agtggtttag taatgaaccg
     5041 gtccaccacc accaccacca ctaaggatcc ggggttgggg ttgcgccttt tccaaggcag
     5101 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     5161 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     5221 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     5281 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     5341 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     5401 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     5461 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     5521 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     5581 gcaattcacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5641 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5701 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5761 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5821 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5881 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5941 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6001 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6061 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6121 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6181 cggtgccgaa ggtagaggtt ctctcctcac ttgtggtgat gttgaagaaa accctggtcc
     6241 aatgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt
     6301 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     6361 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     6421 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     6481 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     6541 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     6601 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     6661 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     6721 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     6781 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     6841 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     6901 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     6961 ttttgacctt gacatgctcc ccgggtaact aagtaaggat cgatccaaga tatcgtattc
     7021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7381 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7561 cgcctgtttc gcctcggcgt ccggactaga ggtaccttta agaccaatga cttacaaggc
     7621 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7681 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7741 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7801 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7861 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7921 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     8101 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctttcaaa tatgtatccg
     8281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9481 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10081 gctcgccgca gccgaacgac cga
//
