LOCUS       sequence-232054-       10120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1 integration plasmid for cuO array with Puro selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, 
            Lomvardas S, Weiner OD
  TITLE     Live-cell imaging reveals enhancer-dependent Sox2 transcription in 
            the absence of enhancer proximity.
  JOURNAL   Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
  PUBMED    31124784
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-24"; volume: "8. pii: 41769. doi"; pages: " 
            10.7554/eLife.41769"
COMMENT     Sequence Label: pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
FEATURES             Location/Qualifiers
     source          1..10120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    78..103
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    114..139
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    150..175
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    186..211
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    293..318
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    329..354
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    365..390
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    401..426
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    437..462
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    473..498
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    509..534
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    546..571
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    582..607
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    618..643
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    654..679
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    690..715
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    726..751
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    762..787
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    798..823
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    834..859
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    870..895
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    941..966
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    977..1002
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1013..1038
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1049..1074
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1085..1110
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1121..1146
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1157..1182
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1194..1219
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1230..1255
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1266..1291
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1302..1327
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1338..1363
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1374..1399
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1410..1435
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1446..1471
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1482..1507
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1518..1543
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1589..1614
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1625..1650
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1661..1686
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1697..1722
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1733..1758
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1769..1794
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1805..1830
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1842..1867
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1878..1903
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1914..1939
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1950..1975
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1986..2011
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2022..2047
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2058..2083
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2094..2119
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2130..2155
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2166..2191
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2237..2262
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2273..2298
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2309..2334
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2345..2370
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2381..2406
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2417..2442
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2453..2478
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2490..2515
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2526..2551
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2562..2587
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2598..2623
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2634..2659
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2713..2738
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2749..2774
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2785..2810
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2821..2846
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2857..2882
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2928..2953
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2964..2989
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3000..3025
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3036..3061
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3072..3097
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3108..3133
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3144..3169
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3181..3206
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3217..3242
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3253..3278
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3289..3314
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3325..3350
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3361..3386
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3397..3422
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3433..3458
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3469..3494
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3505..3530
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3576..3601
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3612..3637
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3648..3673
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3684..3709
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3720..3745
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3756..3781
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3792..3817
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3829..3854
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3865..3890
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3901..3926
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3937..3962
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    3973..3998
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4009..4034
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4045..4070
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4081..4106
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4117..4142
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4153..4178
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4224..4249
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4260..4285
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4296..4321
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4332..4357
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4368..4393
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4404..4429
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4440..4465
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4477..4502
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4513..4538
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4549..4574
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4585..4610
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4621..4646
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4657..4682
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4693..4718
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4729..4754
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4765..4790
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4801..4826
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4872..4897
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4908..4933
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4944..4969
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    4980..5005
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5016..5041
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5052..5077
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5088..5113
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5125..5150
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5198..5223
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5234..5259
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    5270..5295
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P1 operator sequence from the p-cym 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    complement(5313..5337)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     5360..5376
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5361..5377
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5430..5479
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    complement(5484..5517)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6025..6042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6196..6784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6276..6295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6955..7746,7747..7815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7578..7597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7816..7920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7947..8402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8034..8053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8244..8265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8611..9110
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8646..8669)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9043..9062
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9122..9169
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9188..9787
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9188..9207)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9684..9704
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(9819..9836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9825..10049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(10070..10103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(10120,1..24)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
       61 gggccccccc tcgagaaaga aacaaaccaa cctgtctgta ttatcaagcc tgataataca
      121 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
      181 cctgataata cagacaggtt ggtttgtttc tttktckcag ataatwcaga caggttggtt
      241 tgtttcttta agcctgataa tacagacagt tggtttgttt ctttttctca gataatacag
      301 acaggttggt ttgtttcttt aagcctgata atacagacag gttggtttgt ttctttttct
      361 cagataatac agacaggttg gtttgtttct ttaagcctga taatacagac aggttggttt
      421 gtttcttttt ctcagataat acagacaggt tggtttgttt ctttaagcct gataatacag
      481 acaggttggt ttgtttcttt ttctcagata atacagacag gttggtttgt ttcttttaag
      541 cctgataata cagacaggtt ggtttgtttc tttttctcag ataatacaga caggttggtt
      601 tgtttcttta agcctgataa tacagacagg ttggtttgtt tctttttctc agataataca
      661 gacaggttgg tttgtttctt taagcctgat aatacagaca ggttggtttg tttctttgtc
      721 gagaaagaaa caaaccaacc tgtctgtatt atcaagcctg ataatacaga caggttggtt
      781 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
      841 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
      901 cctgataata cagacagttg gtttgtttct ttttctcaga taatacagac aggttggttt
      961 gtttctttaa gcctgataat acagacaggt tggtttgttt ctttttctca gataatacag
     1021 acaggttggt ttgtttcttt aagcctgata atacagacag gttggtttgt ttctttttct
     1081 cagataatac agacaggttg gtttgtttct ttaagcctga taatacagac aggttggttt
     1141 gtttcttttt ctcagataat acagacaggt tggtttgttt cttttaagcc tgataataca
     1201 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
     1261 cctgataata cagacaggtt ggtttgtttc tttttctcag ataatacaga caggttggtt
     1321 tgtttcttta agcctgataa tacagacagg ttggtttgtt tctttgtcga gaaagaaaca
     1381 aaccaacctg tctgtattat caagcctgat aatacagaca ggttggtttg tttctttttc
     1441 tcagataata cagacaggtt ggtttgtttc tttaagcctg ataatacaga caggttggtt
     1501 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     1561 gacagttggt ttgtttcttt ttctcagata atacagacag gttggtttgt ttctttaagc
     1621 ctgataatac agacaggttg gtttgtttct ttttctcaga taatacagac aggttggttt
     1681 gtttctttaa gcctgataat acagacaggt tggtttgttt ctttttctca gataatacag
     1741 acaggttggt ttgtttcttt aagcctgata atacagacag gttggtttgt ttctttttct
     1801 cagataatac agacaggttg gtttgtttct tttaagcctg ataatacaga caggttggtt
     1861 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     1921 gacaggttgg tttgtttctt tttctcagat aatacagaca ggttggtttg tttctttaag
     1981 cctgataata cagacaggtt ggtttgtttc tttgtcgaga aagaaacaaa ccaacctgtc
     2041 tgtattatca agcctgataa tacagacagg ttggtttgtt tctttttctc agataataca
     2101 gacaggttgg tttgtttctt taagcctgat aatacagaca ggttggtttg tttctttttc
     2161 tcagataata cagacaggtt ggtttgtttc tttaagcctg ataatacaga cagttggttt
     2221 gtttcttttt ctcagataat acagacaggt tggtttgttt ctttaagcct gataatacag
     2281 acaggttggt ttgtttcttt ttctcagata atacagacag gttggtttgt ttctttaagc
     2341 ctgataatac agacaggttg gtttgtttct ttttctcaga taatacagac aggttggttt
     2401 gtttctttaa gcctgataat acagacaggt tggtttgttt ctttttctca gataatacag
     2461 acaggttggt ttgtttcttt taagcctgat aatacagaca ggttggtttg tttctttttc
     2521 tcagataata cagacaggtt ggtttgtttc tttaagcctg ataatacaga caggttggtt
     2581 tgtttctttt tctcagataa tacagacagg ttggtttgtt tctttaagcc tgataataca
     2641 gacaggttgg tttgtttctt tgtcgaccta gtctagagcg gccgcctagc gtaccgggcc
     2701 ccccctcgag aaagaaacaa accaacctgt ctgtattatc aagcctgata atacagacag
     2761 gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct ttaagcctga
     2821 taatacagac aggttggttt gtttcttttt ctcagataat acagacaggt tggtttgttt
     2881 ctttaagcct gataatacag acagttggtt tgtttctttt tctcagataa tacagacagg
     2941 ttggtttgtt tctttaagcc tgataataca gacaggttgg tttgtttctt tttctcagat
     3001 aatacagaca ggttggtttg tttctttaag cctgataata cagacaggtt ggtttgtttc
     3061 tttttctcag ataatacaga caggttggtt tgtttcttta agcctgataa tacagacagg
     3121 ttggtttgtt tctttttctc agataataca gacaggttgg tttgtttctt ttaagcctga
     3181 taatacagac aggttggttt gtttcttttt ctcagataat acagacaggt tggtttgttt
     3241 ctttaagcct gataatacag acaggttggt ttgtttcttt ttctcagata atacagacag
     3301 gttggtttgt ttctttaagc ctgataatac agacaggttg gtttgtttct ttgtcgagaa
     3361 agaaacaaac caacctgtct gtattatcaa gcctgataat acagacaggt tggtttgttt
     3421 ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata atacagacag
     3481 gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct ttaagcctga
     3541 taatacagac agttggtttg tttctttttc tcagataata cagacaggtt ggtttgtttc
     3601 tttaagcctg ataatacaga caggttggtt tgtttctttt tctcagataa tacagacagg
     3661 ttggtttgtt tctttaagcc tgataataca gacaggttgg tttgtttctt tttctcagat
     3721 aatacagaca ggttggtttg tttctttaag cctgataata cagacaggtt ggtttgtttc
     3781 tttttctcag ataatacaga caggttggtt tgtttctttt aagcctgata atacagacag
     3841 gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct ttaagcctga
     3901 taatacagac aggttggttt gtttcttttt ctcagataat acagacaggt tggtttgttt
     3961 ctttaagcct gataatacag acaggttggt ttgtttcttt gtcgagaaag aaacaaacca
     4021 acctgtctgt attatcaagc ctgataatac agacaggttg gtttgtttct ttttctcaga
     4081 taatacagac aggttggttt gtttctttaa gcctgataat acagacaggt tggtttgttt
     4141 ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata atacagacag
     4201 ttggtttgtt tctttttctc agataataca gacaggttgg tttgtttctt taagcctgat
     4261 aatacagaca ggttggtttg tttctttttc tcagataata cagacaggtt ggtttgtttc
     4321 tttaagcctg ataatacaga caggttggtt tgtttctttt tctcagataa tacagacagg
     4381 ttggtttgtt tctttaagcc tgataataca gacaggttgg tttgtttctt tttctcagat
     4441 aatacagaca ggttggtttg tttcttttaa gcctgataat acagacaggt tggtttgttt
     4501 ctttttctca gataatacag acaggttggt ttgtttcttt aagcctgata atacagacag
     4561 gttggtttgt ttctttttct cagataatac agacaggttg gtttgtttct ttaagcctga
     4621 taatacagac aggttggttt gtttctttgt cgagaaagaa acaaaccaac ctgtctgtat
     4681 tatcaagcct gataatacag acaggttggt ttgtttcttt ttctcagata atacagacag
     4741 gttggtttgt ttctttaagc ctgataatac agacaggttg gtttgtttct ttttctcaga
     4801 taatacagac aggttggttt gtttctttaa gcctgataat acagacagtt ggtttgtttc
     4861 tttttctcag ataatacaga caggttggtt tgtttcttta agcctgataa tacagacagg
     4921 ttggtttgtt tctttttctc agataataca gacaggttgg tttgtttctt twagcctgat
     4981 aatacagaca ggttggtttg tttctttttc tcagataata cagacaggtt ggtttgtttc
     5041 tttaagcctg ataatacaga caggttggtt tgtttctttt tctcagataa tacagacagg
     5101 ttggtttgtt tcttttaagc ctgataatac agacaggttg gtttgtttct ttttctcnag
     5161 ataatacaga caggttkgtt tgtwtywtya agcctgataa tacagacagg ttggtttgtt
     5221 tctttttctc agataataca gacaggttgg tttgtttctt taagcctgat aatacagaca
     5281 ggttggtttg tttctttgtc gacctagtct agacccagct ttcttgtaca aagtggttga
     5341 tggggatccg aattcgagct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
     5401 cagcccgggc agggatccac tagttctagt cggccggctt gtcgacgacg gcggtctccg
     5461 tcgtcaggat catccgggcc tagataactt cgtatagcat acattatacg aagttatgcg
     5521 gcttcgagac cgtgacctac atcgtcgacg cggccagatg gtaagccctc ccgtatcgta
     5581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6001 ccgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7921 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     7981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8101 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8161 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8221 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8281 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8341 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8401 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttk ssyarysmcr
     8521 rsgttktaaa acgacggcca gtgaattcga gctcggtacg agtttacgtc cagccaagct
     8581 agcttggctg caggtcgtcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt
     8641 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     8701 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     8761 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     8821 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     8881 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     8941 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     9001 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     9061 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     9121 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     9181 ctaaaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     9241 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     9301 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     9361 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     9421 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     9481 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     9541 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     9601 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9661 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9721 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     9781 tgcctgaggg gatcaattct ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     9841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     9901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     9961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    10021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca taacttcgta
    10081 tagcatacat tatacgaagt tatgtaccgg gccccatcaa
//
