LOCUS       sequence-232058-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral overexpression of full length PPME1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  TITLE     Westermarck PPME1 expression plasmids
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCSCIGW-PME1
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1150..1173
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1210..1233
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(1250..1824,1825..1827,1828..1836)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1417..1434)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1644..1663
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1837..1839,1840..1842,1843..2556)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1837..1839,1840..1842,1843..2553)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1882..1903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2143..2162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2490..2511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2566..3154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2619..2639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3037..3048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3669..3849
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3875..3892)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3881..4105
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4151..4579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4238..4257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4448..4469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4588..4608)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4593..4922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4773..4908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4835..4854
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4970..5017
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5036..5410
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5540..5661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5577..5596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5631..5650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5710..5726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5710..5726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5723..5745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5734..5750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5758..5764,5765..5782,5783..5788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5803..5824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5941..5958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6112..6700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6192..6211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6871..7662,7663..7731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7494..7513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7732..7836)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7911..7930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8102..8481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8483..8681
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8699..8879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8926..9051
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9544..9777
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9962..10006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10260..10377
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10458..10761
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10762..10965
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atgcacctcg gccgccttcc ctctcgccca cctctacccg gcagcggggg cagtcagagc
       61 ggagccaaga tgcgaatggg ccctggaaga aagcgggact tttcccctgt tccttggagt
      121 cagtattttg agtccatgga agatgtagaa gtagagaatg aaactggcaa ggatactttt
      181 cgagtctaca agagtggttc agagggtcca gtcctgctcc ttctgcatgg aggaggtcat
      241 tctgcccttt cttgggctgt gttcacggca gcgattatta gtagagttca gtgtaggatt
      301 gtagctttgg atctgcgaag tcatggtgaa acaaaggtca agaatcctga agatctgtct
      361 gcagaaacaa tggcaaaaga cgttggcaat gtggttgaag ccatgtatgg ggaccttcct
      421 cctccaatta tgctgattgg acatagcatg ggtggtgcta ttgcagtcca cacagcatca
      481 tccaacctgg taccaagcct cttgggtctg tgcatgattg atgttgtaga aggtacagct
      541 atggatgcac ttaatagcat gcagaatttc ttacggggtc gtcctaaaac cttcaagtct
      601 ctggagaatg ctattgaatg gagtgtgaag agtggccaga ttcgaaatct ggagtctgcc
      661 cgtgtctcaa tggttggcca agtcaaacag tgtgaaggaa ttacaagtcc agaaggctca
      721 aaatctatag tggaaggaat catagaggaa gaagaagaag atgaggaagg aagtgagtct
      781 ataagcaaga ggaaaaagga agatgacatg gagaccaaga aagaccatcc atacacctgg
      841 agaattgaac tggcaaaaac agaaaaatac tgggacggct ggttccgagg cttatccaat
      901 ctctttctta gttgtcccat tcctaaattg ctgctcttgg ctggtgttga tagattggat
      961 aaagatctga ccattggcca gatgcaaggg aagttccaga tgcaggtcct accccagtgt
     1021 ggccatgcag tccatgagga tgcccctgac aaggtagctg aagctgttgc cactttcctg
     1081 atccggcaca ggtttgcaga acccatcggt ggattccagt gtgtgtttcc tggctgtgga
     1141 tccgctagct ggagccaccc gcagttcgag aaaggtggag gttccggagg tggatcggga
     1201 ggtggatcgt ggagccaccc gcagttcgaa aaaggcgcct aaaccggtgc ccctctccct
     1261 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     1321 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     1381 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     1441 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     1501 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     1561 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     1621 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     1681 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta
     1741 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     1801 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg
     1861 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1921 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1981 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2041 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2101 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2161 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2221 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2281 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2341 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2401 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2461 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2521 gggatcactc tcggcatgga cgagctgtac aagtaactcg aggataatca acctctggat
     2581 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2641 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2701 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2761 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2821 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2881 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2941 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3001 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3061 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3121 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg tcgagaccta gaaaaacatg
     3181 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     3241 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     3301 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3361 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     3421 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     3481 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     3541 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     3601 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     3661 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3721 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3781 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3841 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     3901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4081 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4141 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4561 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4861 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4921 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     4981 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     5041 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     5101 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     5161 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     5221 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     5281 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     5341 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     5401 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5461 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5521 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5641 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5701 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5761 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5881 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5941 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6301 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6541 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6781 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6841 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6901 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6961 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7021 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7081 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7141 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7201 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7261 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7321 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7381 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7441 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7501 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7561 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7621 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7681 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7741 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7801 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7861 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     7921 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     7981 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     8041 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     8101 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     8161 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8221 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     8281 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     8341 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     8401 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8461 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     8521 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8581 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8641 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     8701 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8761 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8821 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8881 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8941 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9001 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9061 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9121 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9181 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9241 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9301 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9361 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9421 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9481 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9541 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9601 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9661 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9721 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     9781 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     9841 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     9901 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9961 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10021 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10081 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10141 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10201 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcttaact
    10261 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10321 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttgga
    10381 tcccccgggc tgcaggaatt cgattaatag taatcaatta cggggtcatt agttcatagc
    10441 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    10501 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    10561 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10621 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10681 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10741 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
    10801 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
    10861 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
    10921 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
    10981 cagatccgct agcgcttgtc ggccctcgaa aagagc
//
