LOCUS       sequence-232066-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral overexpression of full length PPME1 with mutation in the 
            PP2Ac binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  TITLE     Westermarck PPME1 expression plasmids
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCSCIGW-PME1 R369D
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1709..1732
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1769..1792
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(1809..2383,2384..2386,2387..2395)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1976..1993)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2203..2222
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2396..2398,2399..2401,2402..3115)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2396..2398,2399..2401,2402..3112)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2441..2462)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2702..2721)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3049..3070
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3125..3713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3178..3198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3596..3607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4228..4408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4434..4451)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4440..4664
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4710..5138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4797..4816)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5007..5028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5147..5167)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5152..5481
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5332..5467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5394..5413
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5529..5576
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5595..5969
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6099..6220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6136..6155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6190..6209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6269..6285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6269..6285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6282..6304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6293..6309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6317..6323,6324..6341,6342..6347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6362..6383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6500..6517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6671..7259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6751..6770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7430..8221,8222..8290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8053..8072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8291..8395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8470..8489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8661..9040
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9042..9240
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9258..9438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9485..9610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10103..10336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10521..10565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10819..10936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgctt
      541 gtcggccctc gaaaagagca tgcacctcgg ccgccttccc tctcgcccac ctctacccgg
      601 cagcgggggc agtcagagcg gagccaagat gcgaatgggc cctggaagaa agcgggactt
      661 ttcccctgtt ccttggagtc agtattttga gtccatggaa gatgtagaag tagagaatga
      721 aactggcaag gatacttttc gagtctacaa gagtggttca gagggtccag tcctgctcct
      781 tctgcatgga ggaggtcatt ctgccctttc ttgggctgtg ttcacggcag cgattattag
      841 tagagttcag tgtaggattg tagctttgga tctgcgaagt catggtgaaa caaaggtcaa
      901 gaatcctgaa gatctgtctg cagaaacaat ggcaaaagac gttggcaatg tggttgaagc
      961 catgtatggg gaccttcctc ctccaattat gctgattgga catagcatgg gtggtgctat
     1021 tgcagtccac acagcatcat ccaacctggt accaagcctc ttgggtctgt gcatgattga
     1081 tgttgtagaa ggtacagcta tggatgcact taatagcatg cagaatttct tacggggtcg
     1141 tcctaaaacc ttcaagtctc tggagaatgc tattgaatgg agtgtgaaga gtggccagat
     1201 tcgaaatctg gagtctgccc gtgtctcaat ggttggccaa gtcaaacagt gtgaaggaat
     1261 tacaagtcca gaaggctcaa aatctatagt ggaaggaatc atagaggaag aagaagaaga
     1321 tgaggaagga agtgagtcta taagcaagag gaaaaaggaa gatgacatgg agaccaagaa
     1381 agaccatcca tacacctgga gaattgaact ggcaaaaaca gaaaaatact gggacggctg
     1441 gttccgaggc ttatccaatc tctttcttag ttgtcccatt cctaaattgc tgctcttggc
     1501 tggtgttgat agattggata aagatctgac cattggccag atgcaaggga agttccagat
     1561 gcaggtccta ccccagtgtg gccatgcagt ccatgaggat gcccctgaca aggtagctga
     1621 agctgttgcc actttcctga tcgaccacag gtttgcagaa cccatcggtg gattccagtg
     1681 tgtgtttcct ggctgtggat ccgctagctg gagccacccg cagttcgaga aaggtggagg
     1741 ttccggaggt ggatcgggag gtggatcgtg gagccacccg cagttcgaaa aaggcgccta
     1801 aaccggtgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     1861 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     1921 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     1981 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2041 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2101 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2161 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2221 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2281 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     2341 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     2401 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2461 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2521 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2581 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2641 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2701 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2761 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2821 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2881 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2941 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3001 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3061 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaactcga
     3121 ggataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     3541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3661 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgggt
     3721 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     3781 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     3841 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     3901 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     3961 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4021 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     4081 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     4141 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     4201 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     4261 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4321 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4381 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     4441 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4501 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4561 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4621 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4681 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4741 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4801 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4861 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4921 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4981 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5041 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5101 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5161 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5221 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5281 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5341 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5401 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5461 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5521 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     5581 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     5641 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     5701 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     5761 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     5821 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     5881 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     5941 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     6001 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6061 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6121 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6181 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6241 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6481 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7081 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8401 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     8461 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8521 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8581 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     8641 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     8701 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     8761 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     8821 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     8881 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     8941 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     9001 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     9061 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9121 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     9181 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9241 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9301 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9361 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9421 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9481 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9541 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9601 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     9661 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     9721 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     9781 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     9841 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     9901 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     9961 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    10021 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10081 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10141 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10201 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10261 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10321 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    10381 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    10441 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    10501 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    10561 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    10621 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    10681 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    10741 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    10801 tagtgaacgg atcttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10861 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10921 aaaaattcaa aattttggat cccccgggct gcaggaattc gattaatagt aatcaattac
    10981 ggggtcatta gttcatagcc catatatgga gttccg
//
