LOCUS       sequence-232067-        5647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr
  TITLE     Proteins of transcription factor ISGF-3: one gene encodes the 91-and
            84-kDa ISGF-3 proteins that are activated by interferon alpha.
  JOURNAL   Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7836-9.
  PUBMED    1502203
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1992-08-15"; volume: "89(16)"; pages: 
            "7836-9"
COMMENT     Sequence Label: Stat2 pBluescript SK (-)
FEATURES             Location/Qualifiers
     source          1..5647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..95
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     87..104
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     88..104
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     154..170
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     155..171
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2893..2909)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2893..2909)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2946..2964)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2985..3001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2985..3001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2998..3020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3009..3025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3033..3039,3040..3057,3058..3063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3078..3099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3216..3233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3387..3975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3467..3486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4146..4937,4938..5006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4769..4788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5007..5111)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5138..5593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5225..5244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5435..5456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
       61 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      121 cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg
      181 atatcgaatt ccgactgcaa ccctaatcag agcccaaatg gcgcagtggg aaatgctgca
      241 gaatcttgac agcccctttc aggatcagct gcaccagctt tactcgcaca gcctcctgcc
      301 tgtggacatt cgacagtact tggctgtctg gattgaagac cagaactggc aggaagctgc
      361 acttgggagt gatgattcca aggctaccat gctattcttc cacttcttgg atcagctgaa
      421 ctatgagtgt ggccgttgca gccaggaccc agagtccttg ttgctgcagc acaatttgcg
      481 gaaattctgc cgggacattc agcccttttc ccaggatcct acccagttgg ctgagatgat
      541 ctttaacctc cttctggaag aaaaaagaat tttgatccag gctcagaggg cccaattgga
      601 acaaggagag ccagttctcg aaacacctgt ggagagccag caacatgaga ttgaatcccg
      661 gatcctggat ttaagggcta tgatggagaa gctggtaaaa tccatcagcc aactgaaaga
      721 ccagcaggat gtcttctgct tccgatataa gatccaggcc aaagggaaga caccctctct
      781 ggacccccat cagaccaaag agcagaagat tctgcaggaa actctcaatg aactggacaa
      841 aaggagaaag gaggtgctgg atgcctccaa agcactgcta ggccgattaa ctaccctaat
      901 cgagctactg ctgccaaagt tggaggagtg gaaggcccag cagcaaaaag cctgcatcag
      961 agctcccatt gaccacgggt tggaacagct ggagacatgg ttcacagctg gagcaaagct
     1021 gttgtttcac ctgaggcagc tgctgaagga gctgaaggga ctgagttgcc tggttagcta
     1081 tcaggatgac cctctgacca aaggggtgga cctacgcaac gcccaggtca cagagttgct
     1141 acagcgtctg ctccacagag cctttgtggt agaaacccag ccctgcatgc cccaaactcc
     1201 ccatcgaccc ctcatcctca agactggcag caagttcacc gtccgaacaa ggctgctggt
     1261 gagactccag gaaggcaatg agtcactgac tgtggaagtc tccattgaca ggaatcctcc
     1321 tcaattacaa ggcttccgga agttcaacat tctgacttca aaccagaaaa ctttgacccc
     1381 cgagaagggg cagagtcagg gtttgatttg ggactttggt tacctgactc tggtggagca
     1441 acgttcaggt ggttcaggaa agggcagcaa taaggggcca ctaggtgtga cagaggaact
     1501 gcacatcatc agcttcacgg tcaaatatac ctaccagggt ctgaagcagg agctgaaaac
     1561 ggacaccctc cctgtggtga ttatttccaa catgaaccag ctctcaattg cctgggcttc
     1621 agttctctgg ttcaatttgc tcagcccaaa ccttcagaac cagcagttct tctccaaccc
     1681 ccccaaggct ccctggagct tgctgggccc tgctctcagt tggcagttct cctcctatgt
     1741 tggccgaggc ctcaactcag accagctgag catgctgaga aacaagctgt tcgggcagaa
     1801 ctgtaggact gaggatccat tattgtcctg ggctgacttc actaagcgag agagccctcc
     1861 tggcaagtta ccattctgga catggctgga caaaattctg gagttagtac atgaccacct
     1921 gaaggatctc tggaatgatg gacgcatcat gggctttgtg agtcggagcc aggagcgccg
     1981 gctgctgaag aagaccatgt ctggcacctt tctactgcgc ttcagtgaat cgtcagaagg
     2041 gggcattacc tgctcctggg tggagcacca ggatgatgac aaggtgctca tctactctgt
     2101 gcaaccgtac acgaaggagg tgctgcagtc actcccgctg actgaaatca tccgccatta
     2161 ccagttgctc actgaggaga atatacctga aaacccactg cgcttcctct atccccgaat
     2221 cccccgggat gaagcttttg ggtgctacta ccaggagaaa gttaatctcc aggaacggag
     2281 gaaatacctg aaacacaggc tcattgtggt ctctaataga caggtggatg aactgcaaca
     2341 accgctggag cttaagccag agccagagct ggagtcatta gagctggaac tagggctggt
     2401 gccagagcca gagctcagcc tggacttaga gccactgctg aaggcagggc tggatctggg
     2461 gccagagcta gagtctgtgc tggagtccac tctggagcct gtgatagagc ccacactatg
     2521 catggtatca caaacagtgc cagagccaga ccaaggacct gtatcacagc cagtgccaga
     2581 gccagatttg ccctgtgatc tgagacattt gaacactgag ccaatggaaa tcttcagaaa
     2641 ctgtgtaaag attgaagaaa tcatgccgaa tggtgaccca ctgttggctg gccagaacac
     2701 cgtggatgag gtttacgtct cccgccccag ccacttctac actgatggac ccttgatgcc
     2761 ttctgacttc taggaaccac atttcctctg ttcttttcat atctcttgcc cttcctactc
     2821 ctcatagcat gatattgttc tccaaggatg ggaatcaggc atgtgtccct tcggaattcc
     2881 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     2941 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     3001 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3061 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     5161 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5221 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5281 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5341 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5401 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5461 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5521 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5581 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     5641 cgggcct
//
