LOCUS       sequence-232085-       10888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP42
FEATURES             Location/Qualifiers
     source          1..10888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             351..867
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    931..1272
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1339..1755
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1685..1707
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1723..1739
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1770..2149
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2150..2353
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2304..2324
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2355..2373
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2376..2394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2404..2428
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2501..2510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2510..2533
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2546..2572
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2588..2612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6561..6585)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6604..7103
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6639..6662)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7036..7055
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7124..7723
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7124..7143)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7620..7640
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    8309..8325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8333..8339,8340..8357,8358..8363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8378..8399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8516..8533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8687..9275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8767..8786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9446..10237,10238..10306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10069..10088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10307..10411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10479..10497
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10535..10557)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10657..10676
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(10870..10888,1..4)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
       61 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
      121 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
      181 atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata
      241 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
      301 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
      361 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
      421 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
      481 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
      541 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
      601 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
      661 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
      721 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct
      781 tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt
      841 gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc
      901 cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc
      961 tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct gtactagtta
     1021 gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc
     1081 cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga
     1141 agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac
     1201 ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg
     1261 tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg
     1321 tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac
     1381 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
     1441 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
     1501 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
     1561 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
     1621 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
     1681 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
     1741 ccttctctag gcgccggaat tagatccttg acattgatta ttgactagtt attaatagta
     1801 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     1861 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     1921 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     1981 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2041 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2101 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2161 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2221 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2281 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2341 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2401 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2461 ccatagaaga caccgggacc gatccagcct ccggggatct gccaccatgg actacaagga
     2521 tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg ctgctcgagg
     2581 ttatcaaaca agtttgtaca aaaaagttgg catgaccata gttgacaaag cttctgaatc
     2641 ttcagaccca tcagcctatc agaatcagcc tggcagctcc gaggcagtct cacctggaga
     2701 catggatgca ggttctgcca gctggggtgc tgtgtcttca ttgaatgatg tgtcaaatca
     2761 cacactttct ttaggaccag tacctggtgc tgtagtttat tcgagttcat ctgtacctga
     2821 taaatcaaaa ccatcaccac aaaaggatca agccctaggt gatggcatcg ctcctccaca
     2881 gaaagttctt ttcccatctg agaagatttg tcttaagtgg caacaaactc atagagttgg
     2941 agctgggctc cagaatttgg gcaatacctg ttttgccaat gcagcactgc agtgtttaac
     3001 ctacacacca cctcttgcca attacatgct atcacatgaa cactccaaaa catgtcatgc
     3061 agaaggcttt tgtatgatgt gtacaatgca agcacatatt acccaggcac tcagtaatcc
     3121 tggggacgtt attaaaccaa tgtttgtcat caatgagatg cggcgtatag ctaggcactt
     3181 ccgttttgga aaccaagaag atgcccatga attccttcaa tacactgttg atgctatgca
     3241 gaaagcatgc ttgaatggca gcaataaatt agacagacac acccaggcca ccactcttgt
     3301 ttgtcagata tttggaggat acctaagatc tagagtcaaa tgtttaaatt gcaagggcgt
     3361 ttcagatact tttgatccat atcttgatat aacattggag ataaaggctg ctcagagtgt
     3421 caacaaggca ttggagcagt ttgtgaagcc ggaacagctt gatggagaaa actcgtacaa
     3481 gtgcagcaag tgtaaaaaga tggttccagc ttcaaagagg ttcactatcc atagatcctc
     3541 taatgttctt acactttctc tgaaacgttt tgcaaatttt accggtggaa aaattgctaa
     3601 ggatgtgaaa taccctgagt atcttgatat tcggccatat atgtctcaac ccaacggaga
     3661 gccaattgtc tacgtcttgt atgcagtgct ggtccacact ggttttaatt gccatgctgg
     3721 ccattacttc tgctacataa aagctagcaa tggcctctgg tatcaaatga atgactccat
     3781 tgtatctacc agtgatatta gatcggtact cagccaacaa gcctatgtgc tcttttatat
     3841 caggtcccat gatgtgaaaa atggaggtga acttactcat cccacccata gccccggcca
     3901 gtcctctccc cgccccgtca tcagtcagcg ggttgtcacc aacaaacagg ctgcgccagg
     3961 ctttatcgga ccacagcttc cctctcacat gataaagaat ccacctcact taaatgggac
     4021 tggaccattg aaagacacgc caagcagttc catgtcgagt cctaacggga attccagtgt
     4081 caacagggct agtcctgtta atgcttcagc ttctgtccaa aactggtcag ttaataggtc
     4141 ctcagtgatc ccagaacatc ctaagaaaca aaaaattaca atcagtattc acaacaagtt
     4201 gcctgttcgc cagtgtcagt ctcaacctaa ccttcatagt aattctttgg agaaccctac
     4261 caagcccgtt ccctcttcta ccattaccaa ttctgcagta cagtctacct cgaacgcatc
     4321 tacgatgtca gtttctagta aagtaacaaa accgatcccc cgcagtgaat cctgctccca
     4381 gcccgtgatg aatggcaaat ccaagctgaa ctccagcgtg ctggtgccct atggcgccga
     4441 gtcctctgag gactctgacg aggagtcaaa ggggctgggc aaggagaatg ggattggtac
     4501 gattgtgagc tcccactctc ccggccaaga tgccgaagat gaggaggcca ctccgcacga
     4561 gcttcaagaa cccatgaccc taaacggtgc taatagtgca gacagcgaca gtgacccgaa
     4621 agaaaacggc ctagcgcctg atggtgccag ctgccaaggc cagcctgccc tgcactcaga
     4681 aaatcccttt gctaaggcaa acggtcttcc tggaaagttg atgcctgctc ctttgctgtc
     4741 tctcccagaa gacaaaatct tagagacctt caggcttagc aacaaactga aaggctcgac
     4801 ggatgaaatg agtgcacctg gagcagagag gggccctccc gaggaccgcg acgccgagcc
     4861 tcagcctggc agccccgccg ccgaatccct ggaggagcca gatgcggccg ccggcctcag
     4921 cagcaccaag aaggctccgc cgccccgcga tcccggcacc cccgctacca aagaaggcgc
     4981 ctgggaggcc atggccgtcg cccccgagga gcctccgccc agcgccggcg aggacatcgt
     5041 gggggacaca gcaccccctg acctgtgtga tcccgggagc ttaacaggcg atgcgagccc
     5101 gttgtcccag gacgcaaagg ggatgatcgc ggagggcccg cgggactcgg cgttggcgga
     5161 agccccggaa gggttgagtc cggctccgcc tgcgcggtcg gaggagccct gcgagcagcc
     5221 actccttgtt caccccagcg gggaccacgc ccgggacgct caggacccat cccagagctt
     5281 gggcgcaccc gaggccgcag agcggccgcc agctcctgtg ctggacatgg ccccggccgg
     5341 tcacccggaa ggggacgctg agcctagccc cggcgagagg gtcgaggacg ccgcggcgcc
     5401 gaaagcccca ggcccttccc cagcgaagga gaaaatcggc agcctcagaa aggtggaccg
     5461 aggccactac cgcagccgga gagagcgctc gtccagcggg gagcccgcca gagagagcag
     5521 gagcaagact gagggccacc gtcaccggcg gcgccgcacc tgcccccggg agcgcgaccg
     5581 ccaggaccgc cacgccccgg agcaccaccc cggccacggc gacaggctca gccctggcga
     5641 gcgccgctct ctgggcaggt gcagtcacca ccactcccga caccggagcg gggtggagct
     5701 ggactgggtc agacaccact acaccgaggg cgagcgtggc tggggccggg agaagttcta
     5761 ccccgacagg ccgcgctggg acaggtgccg gtactaccat gacaggtacg ccctgtacgc
     5821 tgcccgggac tggaagccct tccacggcgg ccgcgagcac gagcgggccg ggctgcacga
     5881 gcggccgcac aaggaccaca accggggccg taggggctgc gagccggccc gggagaggga
     5941 gcggcaccgc cccagcagcc cccgcgcagg cgcgccccac gccctcgccc cgcaccccga
     6001 ccgcttctcc cacgacagaa ctgcacttgt agccggagac aactgtaacc tctctgatcg
     6061 gtttcacgaa cacgaaaatg gaaagtcccg gaaacggaga cacgacagtg tggagaacag
     6121 tgacagtcat gttgaaaaga aagcccggag gagcgaacag aaggatcctc tagaagagcc
     6181 taaagcaaag aagcacaaaa aatcaaagaa gaaaaagaaa tccaaagaca aacaccgaga
     6241 ccgcgactcc aggcatcagc aggactcaga cctctcagca gcgtgctctg acgctgacct
     6301 ccacagacac aaaaaaaaga agaagaaaaa gaagagacat tcaagaaaat cagaggactt
     6361 tgttaaagat tcagaactgc acttacccag ggtcaccagc ttggagactg tcgcccagtt
     6421 ccggggagcc cagggtggct ttcctctctc tggtggcccg cctctggaag gcgtcggacc
     6481 tttccgtgag aaaacgaaac acttacggat ggaaagcagg gatgacaggt gtcgtctctt
     6541 tgagtatggc cagggtgatt gaccaacttt cttgtacaaa gtggttcgat aacgaattct
     6601 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     6661 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     6721 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     6781 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     6841 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     6901 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     6961 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     7021 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     7081 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     7141 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7201 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7261 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7321 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7381 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7441 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7501 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7561 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7621 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7681 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     7741 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt
     7801 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagagaa
     7861 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     7921 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     7981 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc
     8041 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     8101 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc
     8161 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa
     8221 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc
     8281 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac
     8341 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8401 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8461 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10441 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10501 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    10561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10621 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10681 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10741 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10801 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10861 cagggttttc ccagtcacga cgttgtaa
//
