LOCUS       sequence-232086-        3614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse GDNF In situ hybridization probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Fleming MS, Vysochan A, Paixao S, Niu J, Klein R, Savitt JM, Luo W
  TITLE     Cis and trans RET signaling control the survival and central 
            projection growth of rapidly adapting mechanoreceptors.
  JOURNAL   Elife. 2015 Apr 2;4:e06828. doi: 10.7554/eLife.06828.
  PUBMED    25838128
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-04-2"; volume: "4:e06828. doi"; pages: " 
            10.7554/eLife.06828"
COMMENT     Sequence Label: pGEMT-GDNF
FEATURES             Location/Qualifiers
     source          1..3614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     582..604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     596..613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     597..613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     620..639
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        620..638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1375..1393)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1376..1393)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1411..1427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1411..1427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1424..1446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1435..1451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1459..1465,1466..1483,1484..1489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1504..1525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1642..1659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1813..2401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1893..1912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2572..3363,3364..3432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3195..3214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3433..3537)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca
      661 tgctcccggc cgccatggcc gcgggattct gcctggtgtt gctccacacc gcgtctgcct
      721 tcccgctgcc cgccggtaag aggcttctcg aagcgcccgc tgaagaccac tccctcggcc
      781 accgccgcgt gcccttcgcg ctgaccagtg actccaatat gcctgaagat tatcctgacc
      841 agtttgatga cgtcatggat tttattcaag ccaccattaa aagactgaaa aggtcaccag
      901 ataaacaagc ggcagcgctt cctcgaagag agaggaatcg gcaggctgca gctgccagcc
      961 cagagaattc cagagggaaa ggtcgcagag gccagagggg caaaaatcgg gggtgcgttt
     1021 taactgccat acacttaaat gtcactgact tgggtttggg ctatgaaacc aaggaggaac
     1081 tgatctttcg atattgcagc ggttcctgtg aatcggccga gacaatgtat gacaaaatac
     1141 taaaaaacct gtctcggagt agaaggctaa caagtgacaa agtaggccag gcatgttgca
     1201 ggccggtcgc cttcgacgac gacctgtcgt ttttagatga caacctggtt taccatattc
     1261 taagaaagca ttccgctaaa cggtgtggat gtatctgacc aatcactagt gcggccgcct
     1321 gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata
     1381 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1441 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1501 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1561 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1621 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1681 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1741 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1801 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1861 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1921 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1981 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2041 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2101 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2161 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2221 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2281 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2341 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2401 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2461 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2521 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2581 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2641 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2701 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2761 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2821 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2881 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2941 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3001 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3061 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3121 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3181 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3241 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3301 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3361 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3421 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3481 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3541 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3601 aataccgcat cagg
//
