LOCUS       sequence-232089-        9759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conversion of C to T in wheat plants or monocotyledons protoplasts 
            without uracil glycosylase inhibitor (UGI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
  TITLE     Efficient C-to-T base editing in plants using a fusion of nCas9 and 
            human APOBEC3A.
  JOURNAL   Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
  PUBMED    30272679
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261."
COMMENT     Sequence Label: A3A-PBE-Delta-UGI
FEATURES             Location/Qualifiers
     source          1..9759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2669..2692,2693..2695,2696..6769)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6770..6817
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    6875..7051
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7605..7621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7605..7621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7618..7640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7629..7645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7653..7659,7660..7677,7678..7683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7698..7719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7836..7853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8007..8595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8087..8106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8766..9557,9558..9626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9389..9408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9627..9731)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg gaggccagcc cggctagcgg
     2041 cccaaggcat ctcatggacc cgcacatctt caccagcaac ttcaacaacg gcatcggcag
     2101 gcacaagacc tacttgtgct acgaggtgga gaggctcgac aacggaacct ccgtgaagat
     2161 ggaccaacac agggggttcc tccacaacca agccaagaac ctcctctgcg gcttctacgg
     2221 caggcacgcc gagttgaggt tcctcgactt ggtgccatcc ctccaactcg atccagccca
     2281 aatctaccgc gtgacctggt tcatctcctg gtccccatgc ttctcctggg gttgcgccgg
     2341 cgaggttcgg gctttcctcc aagaaaacac ccacgtccgc ctccgcattt tcgccgccag
     2401 gatctatgat tacgaccctc tctacaagga ggccctccag atgctgcggg acgccggtgc
     2461 tcaggtgagt atcatgacct acgacgagtt caagcactgc tgggacacct tcgttgacca
     2521 ccagggctgc ccattccaac catgggacgg tctggatgaa cacagccaag ccttgtccgg
     2581 caggctccgg gccatcctcc aaaaccaggg gaactccggg agcgagacgc caggcacctc
     2641 cgagtcggcc accccagaat ctcttaagga caagaagtac tcgatcggcc tcgccatcgg
     2701 gacgaactca gttggctggg ccgtgatcac cgacgagtac aaggtgccct ctaagaagtt
     2761 caaggtcctg gggaacaccg accgccattc catcaagaag aacctcatcg gcgctctcct
     2821 gttcgacagc ggggagaccg ctgaggctac gaggctcaag agaaccgcta ggcgccggta
     2881 cacgagaagg aagaacagga tctgctacct ccaagagatt ttctccaacg agatggccaa
     2941 ggttgacgat tcattcttcc accgcctgga ggagtctttc ctcgtggagg aggataagaa
     3001 gcacgagcgg catcccatct tcggcaacat cgtggacgag gttgcctacc acgagaagta
     3061 ccctacgatc taccatctgc ggaagaagct cgtggactcc accgataagg cggacctcag
     3121 actgatctac ctcgctctgg cccacatgat caagttccgc ggccatttcc tgatcgaggg
     3181 ggatctcaac ccagacaaca gcgatgttga caagctgttc atccaactcg tgcagaccta
     3241 caaccaactc ttcgaggaga acccgatcaa cgcctctggc gtggacgcga aggctatcct
     3301 gtccgcgagg ctctcgaagt ccaggaggct ggagaacctg atcgctcagc tcccaggcga
     3361 gaagaagaac ggcctgttcg ggaacctcat cgctctcagc ctggggctca ccccgaactt
     3421 caagtcgaac ttcgatctcg ctgaggacgc caagctgcaa ctctccaagg acacctacga
     3481 cgatgacctc gataacctcc tggcccagat cggcgatcaa tacgcggacc tgttcctcgc
     3541 tgccaagaac ctgtcggacg ccatcctcct gtcagatatc ctccgcgtga acaccgagat
     3601 cacgaaggct ccactctctg cctccatgat caagcgctac gacgagcacc atcaggatct
     3661 gaccctcctg aaggcgctgg tccgccaaca gctcccggag aagtacaagg agattttctt
     3721 cgatcagtcg aagaacggct acgctgggta catcgacggc ggggcctcac aagaggagtt
     3781 ctacaagttc atcaagccaa tcctggagaa gatggacggc acggaggagc tcctggtgaa
     3841 gctcaacagg gaggacctcc tgcggaagca gagaaccttc gataacggca gcatccccca
     3901 ccaaatccat ctcggggagc tgcacgccat cctgagaagg caagaggact tctacccttt
     3961 cctcaaggat aaccgggaga agatcgagaa gatcctgacc ttcagaatcc catactacgt
     4021 cggccctctc gcgcggggga actcaagatt cgcttggatg acccgcaagt ctgaggagac
     4081 catcacgccg tggaacttcg aggaggtggt ggacaagggc gctagcgctc agtcgttcat
     4141 cgagaggatg accaacttcg acaagaacct gcccaacgag aaggtgctcc ctaagcactc
     4201 gctcctgtac gagtacttca ccgtctacaa cgagctcacg aaggtgaagt acgtcaccga
     4261 gggcatgcgc aagccagcgt tcctgtccgg ggagcagaag aaggctatcg tggacctcct
     4321 gttcaagacc aaccggaagg tcacggttaa gcaactcaag gaggactact tcaagaagat
     4381 cgagtgcttc gattcggtcg agatcagcgg cgttgaggac cgcttcaacg ccagcctcgg
     4441 gacctaccac gatctcctga agatcatcaa ggataaggac ttcctggaca acgaggagaa
     4501 cgaggatatc ctggaggaca tcgtgctgac cctcacgctg ttcgaggaca gggagatgat
     4561 cgaggagcgc ctgaagacgt acgcccatct cttcgatgac aaggtcatga agcaactcaa
     4621 gcgccggaga tacaccggct gggggaggct gtcccgcaag ctcatcaacg gcatccggga
     4681 caagcagtcc gggaagacca tcctcgactt cctcaagagc gatggcttcg ccaacaggaa
     4741 cttcatgcaa ctgatccacg atgacagcct caccttcaag gaggatatcc aaaaggctca
     4801 agtgagcggc cagggggact cgctgcacga gcatatcgcg aacctcgctg gctcccccgc
     4861 gatcaagaag ggcatcctcc agaccgtgaa ggttgtggac gagctcgtga aggtcatggg
     4921 ccggcacaag cctgagaaca tcgtcatcga gatggccaga gagaaccaaa ccacgcagaa
     4981 ggggcaaaag aactctaggg agcgcatgaa gcgcatcgag gagggcatca aggagctggg
     5041 gtcccaaatc ctcaaggagc acccagtgga gaacacccaa ctgcagaacg agaagctcta
     5101 cctgtactac ctccagaacg gcagggatat gtacgtggac caagagctgg atatcaaccg
     5161 cctcagcgat tacgacgtcg atcatatcgt tccccagtct ttcctgaagg atgactccat
     5221 cgacaacaag gtcctcacca ggtcggacaa gaaccgcggc aagtcagata acgttccatc
     5281 tgaggaggtc gttaagaaga tgaagaacta ctggaggcag ctcctgaacg ccaagctgat
     5341 cacgcaaagg aagttcgaca acctcaccaa ggctgagaga ggcgggctct cagagctgga
     5401 caaggccggc ttcatcaagc ggcagctggt cgagaccaga caaatcacga agcacgttgc
     5461 gcaaatcctc gactctcgga tgaacacgaa gtacgatgag aacgacaagc tgatcaggga
     5521 ggttaaggtg atcaccctga agtctaagct cgtctccgac ttcaggaagg atttccagtt
     5581 ctacaaggtt cgcgagatca acaactacca ccatgcccat gacgcttacc tcaacgctgt
     5641 ggtcggcacc gctctgatca agaagtaccc aaagctggag tccgagttcg tgtacgggga
     5701 ctacaaggtt tacgatgtgc gcaagatgat cgccaagtcg gagcaagaga tcggcaaggc
     5761 taccgccaag tacttcttct actcaaacat catgaacttc ttcaagaccg agatcacgct
     5821 ggccaacggc gagatccgga agagaccgct catcgagacc aacggcgaga cgggggagat
     5881 cgtgtgggac aagggcaggg atttcgcgac cgtccgcaag gttctctcca tgccccaggt
     5941 gaacatcgtc aagaagaccg aggtccaaac gggcgggttc tcaaaggagt ctatcctgcc
     6001 taagcggaac agcgacaagc tcatcgccag aaagaaggac tgggacccaa agaagtacgg
     6061 cgggttcgac agccctaccg tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg
     6121 caagtccaag aagctcaaga gcgtgaagga gctcctgggg atcaccatca tggagaggtc
     6181 cagcttcgag aagaacccaa tcgacttcct ggaggccaag ggctacaagg aggtgaagaa
     6241 ggacctgatc atcaagctcc cgaagtactc tctcttcgag ctggagaacg gcaggaagag
     6301 aatgctggct tccgctggcg agctccagaa ggggaacgag ctcgcgctgc caagcaagta
     6361 cgtgaacttc ctctacctgg cttcccacta cgagaagctc aagggcagcc cggaggacaa
     6421 cgagcaaaag cagctgttcg tcgagcagca caagcattac ctcgacgaga tcatcgagca
     6481 aatctccgag ttcagcaagc gcgtgatcct cgccgacgcg aacctggata aggtcctctc
     6541 cgcctacaac aagcaccggg acaagcccat cagagagcaa gcggagaaca tcatccatct
     6601 cttcaccctg acgaacctcg gcgctcctgc tgctttcaag tacttcgaca ccacgatcga
     6661 tcggaagaga tacacctcca cgaaggaggt cctggacgcg accctcatcc accagtcgat
     6721 caccggcctg tacgagacga ggatcgacct ctcacaactc ggcggggata agagacccgc
     6781 agcaaccaag aaggcagggc aagcaaagaa gaagaagtag gaattcggta cgctgaaatc
     6841 accagtctct ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag
     6901 tttcccgata agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
     6961 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     7021 aaaaccaaaa tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg
     7081 aatataaacc agacacgaga cgactaaacc tggagcccag acgccgttcg aagctagaag
     7141 taccgcttag gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac
     7201 tcccccgtag gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga
     7261 aggataaggt tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta
     7321 ctatacttat actatacgct aagggaatgc ttgtatttat accctatacc ccctaataac
     7381 cccttatcaa tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc
     7441 atgaataggt ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtt
     7501 cttaactcta aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg
     7561 gggatcgcat gcgatatctc gagatctagc ttggcgtaat catggtcata gctgtttcct
     7621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
