LOCUS       sequence-232098-        5310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpoD5∆::natR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Fleming KE, O'Shea EK
  TITLE     An RpaA-Dependent Sigma Factor Cascade Sets the Timing of Circadian 
            Transcriptional Rhythms in Synechococcus elongatus.
  JOURNAL   Cell Rep. 2018 Dec 11;25(11):2937-2945.e3. doi: 
            10.1016/j.celrep.2018.11.049.
  PUBMED    30540929
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-11- 11"; volume: "25"; issue: "11"; pages: "2937-2945.e3"
COMMENT     Sequence Label: KFP74
FEATURES             Location/Qualifiers
     source          1..5310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     62..84
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     76..93
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     77..93
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2787..2803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2787..2803)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2800..2822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2811..2827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2835..2841,2842..2859,2860..2865))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2880..2901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3018..3035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3189..3777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3269..3288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3948..4739,4740..4808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4571..4590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4809..4913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4981..4999
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5037..5059)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5159..5178
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
       61 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac
      121 tctagaggat ccccgggtac taactcagcg atcggaaact gacgctcttc taggatctgc
      181 agtagctcag tgccaactgc accggtcgct ccaagaattg caacgcgata gccctgtgac
      241 aatgacaacc tcctaacccc aaacagcatc gatagcgaaa gcagacaact ctgcgcccat
      301 tgtctggcgg cgcatcggca cacagagttt tggtaatgac agcttctgta gactcgcgct
      361 tctgtccatc atgccaagac tctaaacctg ctcgagaagc accagcgaaa accagttttg
      421 ctcacagatt ttacgagctg ctgcgtcata cagggaaatg aagagcatga agcgctaaag
      481 agttccgacc cattcctggg aattgcgggg aacctccatc atactcggcg ctgtcgagaa
      541 acaagaatac agccaagcgg ggctggacgc cgcattgaga aagatttttg cagctctaac
      601 agccccaagc cagcaggcaa gcaccctttc cagcgccccc agagcccaga ggctcactca
      661 tagaatcacg attctcctag ggcaaagccg caatggccct ctaggctgga ttccgagtag
      721 gggagtcatt tcagacgagc gatcgccaac aatgcaaact cagcgttgat gccacatgtt
      781 ttctcagaat tcttgatggt tctcaatggt gagctagagg gatacaaaag ttcaagaaac
      841 caagcactga actgggcaaa tatcaaagca acatctgggc tttaccgagg acttacattt
      901 tggccaaaat ctaaagagag ctggggcact ggcaagaatt ttatttttta tagagagact
      961 attttccaga taagcactgg ccttgaagtc gacggcaaat tctcatgttt gacagcttat
     1021 catcgaatta taggaataga gcaaacaagc aaaggaaatt ttgtcaaaat aattttattg
     1081 acaacgtctt attaacgttg atataattta aattttattt gacaaaaatg ggctcgtgtt
     1141 gtacaataaa tgtagtgagg tggatgcaat ggcgatgacg ttgtccgata ttaaaagatc
     1201 gcttgatggg aatttaggta aaaggctgac gttaaaagca aacggtggcc ggatccatat
     1261 gaccaccctg gatgataccg cctaccgcta ccgcaccagc gttcccggtg atgccgaagc
     1321 catcgaagcc ctggatggca gctttaccac cgataccgtg tttcgcgtga ccgccacggg
     1381 tgatggcttt accctgcgcg aagtgcccgt cgatccccct ctgaccaaag tgtttcccga
     1441 tgatgaaagt gatgatgaat cggatgctgg cgaagatggc gatcccgata gccgcacctt
     1501 tgtggcctac ggtgatgatg gcgatctggc tggctttgtg gtggtgagct acagcggctg
     1561 gaatcgccgc ttgaccgtgg aagatattga agtggctccc gaacaccgcg gtcacggcgt
     1621 tggtcgcgct ctgatgggcc tggccaccga atttgctcgc gaacgcggtg ctggccacct
     1681 gtggctggaa gtgaccaacg tgaacgctcc cgctatccac gcctatcgcc gcatgggctt
     1741 caccctgtgt ggcctggata ccgctctgta cgatggcacc gccagtgatg gcgaacaggc
     1801 cctgtacatg agcatgccct gcccctaagg ccggccagcc cgcctaatga gcgggctttt
     1861 ttttctattt ggagttgttg cactagtcgc ttccctgtcc agtcctcctg tctgtcttaa
     1921 gcagcttagg cgcgatcgcc taggctgttt ttttgaccgt ccattgcgtg atcggtgcca
     1981 gcgatttcca gccgagaaag cggccgatcg gttccgctcg ctgcaccgcc acacggatta
     2041 aatctccccc ccagcgctgc tgtccagcca aaacgacttg ctcgctttcg agggtaacgg
     2101 cgttaacaac tagccgccca cccatcggca gcgcttgcca gcattgatcg agcacacctg
     2161 gagcagtgac accgccaccg atgaagatgg catccggttg gggcaagcca ctcagcgtag
     2221 ccggtgctga accggcaacc acttgcaggt tcggaacccc gagggcgatc gcattctgtg
     2281 taatgatttg cagccgctgc ggatgctgct caatggcgat cgctcgacag cggcgatcgc
     2341 tacgcatcca ttcaatgccg atcgacccac agccggcacc cacatcccag agcagttggc
     2401 cgggcagggg agctagcgca gctagcgtga tcgctcgcac ttcccgtttg gtcagttgcc
     2461 catcatggtg gtaggccgca tcaggcagtc cagtcaggcg gggcggcaca atcaagtcgc
     2521 gatcgcgggc atggcattca atcgccacgg tgttgagaga ggcgatcgtg ggctgagacc
     2581 agtgctcggc tatttcacta atttttcgct ccttagggcc gcccagatgt tctaaaaccg
     2641 tgagccgact tgacccccag ccctgctgac agagttgctc tgcgatcgtg gctggagtcg
     2701 agcgatcggc actgagggcc aaaatcttgg cgccaggata aagtagcgcg ttaagtaacg
     2761 cgtaccgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     2821 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2881 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2941 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3001 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4921 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4981 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5041 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5101 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5161 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5221 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     5281 gggcgatcgg tgcgggcctc ttcgctatta
//
