LOCUS       sequence-232102-       13283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express the T679A mutant form of the SOMA FRET sensor with a
            nuclear exclusion signal in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, 
            Zhang S, Krysan PJ
  TITLE     A Forster resonance energy transfer sensor for live-cell imaging of 
            mitogen-activated protein kinase activity in Arabidopsis.
  JOURNAL   Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164.
  PUBMED    30444549
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Nov 16. doi: 10.1111/tpj.14164."
COMMENT     Sequence Label: pSOMA-T679A-NES
FEATURES             Location/Qualifiers
     source          1..13283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             368..1162
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1246..1270
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1370..1392
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1384..1401
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1385..1401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1916..2261
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2204..2224
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     5'UTR           2276..2333
                     /label=AtADH 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the 
                     Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et 
                     al., 2008"
     CDS             join(2349..2351,2352..2354,2355..3062)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             3498..3845
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             3594..3941
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             3690..4037
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             3786..4133
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             3882..4229
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(4485..4487,4488..4490,4491..5003)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             5208..5234
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      5316..5564
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(5586..5602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5586..5602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5599..5621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5610..5626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5634..5640,5641..5658,5659..5664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5679..5700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5835..6014
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5867..6050
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6071..6865
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6125..6144)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6735..6754
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7139..7391
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     7461..7482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7729..7753
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    7760..12395
                     /label=pRi replicator region
                     /note="replicator region from Agrobacterium plasmid pRiA 
                     4b"
     rep_origin      12639..13227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13128..13147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc
       61 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
      121 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcacccata
      181 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
      241 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
      301 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
      361 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
      421 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
      481 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
      541 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
      601 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
      661 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
      721 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
      781 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
      841 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
      901 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
      961 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     1021 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     1081 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     1141 tttactggat gaattgtttt agtcacatac aaatggacga acggataaac cttttcacgc
     1201 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
     1261 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcctggcg
     1321 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     1381 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtccccag attagccttt
     1441 tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtctca
     1501 tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta
     1561 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
     1621 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc
     1681 aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt
     1741 caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac
     1801 agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
     1861 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
     1921 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
     1981 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
     2041 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     2101 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     2161 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     2221 cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact ctagttacat
     2281 cacaatcaca caaaactaac aaaagatcaa aagcaagttc ttcactgttg atacatatgg
     2341 aattcggcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2401 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2461 ccacctacgg caagctgacc ctgaagcttc tatgcaccac cggcaagctg cccgtgccct
     2521 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
     2581 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2641 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2701 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2761 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     2821 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     2881 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2941 acaaccacta cctgagctac cagtccgccc tgttcaaaga ccccaacgag aagcgcgatc
     3001 acatggtcct gctggagttc ctgaccgccg ccgggatcac tgagggcatg aacgagctgt
     3061 acctcgagaa gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg gtcatttgta
     3121 ccacgggtca aattcccatc cgagatttgt cagctgatat ttcacaagtg cttaaggaaa
     3181 aacgatccat aaagaaagtt tggacatttg gtagaaaccc agcctgtgac tatcatttag
     3241 gaaacatttc aagactgtca aataagcatt tccaaatact actaggagaa gacggtaacc
     3301 ttttattgaa tgacatttcc actaatggga cctggttaaa tgggcaaaaa gtcgagaaga
     3361 acagcaatca gttactgtcc caaggtgatg aaataaccgt tggtgtaggc gtggaatcag
     3421 atattttatc tctggtcatt ttcataaacg acaaatttaa gcagtgcctg gagcagaaca
     3481 aagttgatcg cggtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg
     3541 gtggtagtgc tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg
     3601 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca
     3661 gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg
     3721 gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc tggtggtagt gctggtggta
     3781 gtaccagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg
     3841 gtggttccgg cagtgctggt ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg
     3901 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg ttccggcagt gctggtggta
     3961 gtgctggtgg tagtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg
     4021 gtggtagtgc tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg
     4081 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca
     4141 gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg
     4201 gtggtagtgc tggtggtagt gctggtggtt ccggacctgg ttctaagaaa atgttttggc
     4261 ggtctgcctc ttggtctgct tcacggactg catcacaagt tcctgagggt gatgagcaaa
     4321 gcctgaacat tccgtgtgct attagttctg ggccgagtcg aagatgtcca gctgctcctt
     4381 tggcacctcg tgatcatcat aacagcaagg ctagagcttg tttgccacca ttgcagcctc
     4441 ttgccatttc taggaggagc ttagacgagt ggcctggcgg ccgcatggtg agcaagggcg
     4501 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     4561 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     4621 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt
     4681 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     4741 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4801 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4861 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4921 acatcagcgg gaacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     4981 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     5041 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     5101 ccgccttaag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcttga
     5161 ccgccgccgg gatcactctc ggcatggacg agctgtctag actgcagctg cctcctctgg
     5221 aacgcctgac tctggattaa gtcgacaatt ctgaatcaac aactctcctg gcgcaccatc
     5281 gtcggctaca gcctcgggaa ttgctaccga gctcttatga agatgaagat gaaatatttg
     5341 gtgtgtcaaa taaaaagcta gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg
     5401 tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatctcat tataatgaat
     5461 aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttcgc atataactac
     5521 tgtatgtgct atggtatgga ctatggaata tgattaaaga taaggaattg caattcgtaa
     5581 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5641 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5701 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca ggatcatgag
     5761 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac
     5821 gtttggaact gacagaaccg caacgttgaa ggagccactc agccgcgggt ttctggagtt
     5881 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     5941 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     6001 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatctgg
     6061 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6241 gaatgaacta caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6601 tatgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6841 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6901 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6961 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7021 gagttcttcg cccacgggat ctctgcggaa caggcggtcg aaggtgccga tatcattacg
     7081 acagcaacgg ccgacaagca caacgccacg atcctgagcg acaatatgat cgggccccga
     7141 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     7201 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     7261 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     7321 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     7381 gttactagat cgggaatttg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7501 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag
     7561 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     7621 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta
     7681 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     7741 atatatcctg ccacaagcta gctttctgag ccgccgattt tcctcctcga gttggatgaa
     7801 ctcgccgagt tcatcgtcaa ctgaaacaga cacggccgga ttctgtgaga caggttgaac
     7861 cgcagctctc ttccattgat aataggtctg aacggaaata cccacgatct taacggcgtc
     7921 cttcaaggtt gcgccgccag cgacctgagc ttcgatttga ccgatcttct ccagtttttc
     7981 tcggttgctg aggccgcggg ttttcggctt cacggatttg aacgatcccg tgcgggctgt
     8041 ttcggctggt gctttctttg ctcttctacc tctaggagca gccggctcaa cttcggcagc
     8101 agcagtaccg tccggcggat tctggatctc ttcgtcagcc attaatcgtc ctctgtgtgg
     8161 gttattgctt tgtctgccag ctcgatccaa gagtcaacgt ttgtgcctag ggcagtaaat
     8221 aggcagtgct ccgcgactac atgcctcggc cggcaaaata ccgccgcatg tagagcaggc
     8281 tctccttcac gatcaacgat cggcatgggg ccttcgtgct tgttgagtaa tgttatcgct
     8341 cccatcagag cacgcttggt actccgggaa tcggatggtc tgtcgatcat ccaaaaaacg
     8401 ctcatgtttt caacctatta ggtctgtggt cagctgacca cagaccatcc tgctccatac
     8461 tcgctaattc tagccaaacc gcaacgtccc ctgcccgcta gccttcaaga gcgccattat
     8521 catcgggcca agtgaaaact tcccgagttc gctccgccgt gtcagatctc ggagatagcc
     8581 cccaggcgaa ttgatgaagt tcgctcgctc caaaatgcac gccatcgctg ctgccgcatt
     8641 ctccggtccc attgcctcac acgcgtcttg gtaagccgac gggctgaccc ccagcataga
     8701 ccgaaccacc accgcagccg acatgaggtc acgccagcta gcaaccgcac cgctcggccc
     8761 ataattgcca atggtcgggc acgctttcag gatcatcccg agggggaacg cttttatcgg
     8821 ctcgctcctt gcccggtcta tttcactcgg cttagcgccc tgctcctttt cagagcgagg
     8881 ttcaagttca ttaacggatt cgggttttga gttctgtatg tgctgctcgc tctgggcagc
     8941 attggtgcta ttattttctg aattgtctct aatttccaac cggttgatta tctcttcctg
     9001 gagcatccac atctcttcga gaattgactc tacatcagca agcgtcgggg cgcgtgggat
     9061 tctacccaca agttccacat agacttcctc gacagcttgc cagtcgccct ccgctccctc
     9121 ttccatagct gccgtaatta gcttccgaac gtcccgtcgg caaatcgtca gactttcttt
     9181 ggccatcctg aatgctgctc gatcggccat cacctgctgt gccatcatcg ctagctcttc
     9241 ggaccgcgcg agaagcggag acaaatcgaa gccaaacgcg cgctcgatct gaccagcgcc
     9301 atccttacga gcgtaacgct ttccgttggc gctatccttc cggacgatca agcctgactc
     9361 cacgagcatg gcgatgtgcc tacgcaaagt cgcgccagcc atcccatgcg cccgaagggc
     9421 aagctgagca ttcgacggga agacgatcag ctgtgcctcc tgacgcaact ccgtttccgg
     9481 gtgaaagctc aatagcgcat caaggacggc aagactgttg gactggattc caagtagttc
     9541 catggccgcg gacgcgtccc taaagacctt ccacttgtcc gctgtcttgc cttgtttgat
     9601 atcggccagc gccgtctggc gccgcacaag cgcaagcgtc attggccgcc gcccgaatgg
     9661 cgtcgttaca cttcctgtct gcatcatctt tcacctttca gcaggcaaag gaaatcagct
     9721 caccaaaacg gcgctaaaaa ctcttgacga ggattcgagg aaatgcgatt ctgttcgcgc
     9781 tagagagaca gaagggcttc cgcgacggcg acgttgaggg ggctcttttc ttttgcggtt
     9841 tactctcccc gtttccgttg gttctcagcg tggtacgctt gatacagcgc tggcacatga
     9901 tcgagcacga aggtcgcaaa atcgggcgtc gccttcctgt caatcgtgat ttccagtttg
     9961 gccttgctct gcgtcacctg tgcaattctg gtgccgtctg gggtggccat gacctcggga
    10021 agtccacgcg caacccgact gggcttcaga ctagcgatca ccgccttgaa tcgttctgcc
    10081 gatggcagcg cttgaacttc ctccgacata gcatatttag ccacgtcggc cggtgaagaa
    10141 actttctcaa tcagctcggc aagttgttgc caactcggcc gtccaacacc aggagcggca
    10201 ccaatagcat cggtcagttc agaggggagg gcgtcaacga gcagaagcat cttggacaaa
    10261 ttgctcttgt cgatcgacat cgcggcgatg acaatctctc gagaaaactg cctgttcagg
    10321 cgatgtgcga agcgcgcctt ttcgatgaag gtaagatctt cgcgcacatt gttttcctga
    10381 ccctgtgcta cgaccacttg ctcgtccgtc agttcgcgaa cgaccgccct gaccggaagt
    10441 ccgagttctg aaacggcgcg tagccggcgg tggccgaagg caacctgata tcggcccggc
    10501 tggctcggat gcggtcgcac aaggattggg acttgctgtc cttgttcccg gatcgaagta
    10561 aggagcccgt caatgtcccc tcgcatacga tcctgcacga aagacggttc tattgacgag
    10621 gcatccaact ctatcactgc ctgaccttca gcgagacgcc gctcgatctc ttcggcacgg
    10681 ctaagacgat cgttttgctc tcgcagtgcg ttaccaatgt tcgctgtgag cttcgttgcc
    10741 ggatcgcgct ccttccttgt tacgccgagg agcggcatgg agcggttctt tgccgtccta
    10801 ttgtcggcgg gcgacgtctc aggggcgtca gttgagacgc caaggatgtg cttccggctc
    10861 atgtgggcct accccatgct tttttgatca gtgtttcgat ctcgtcgttg acggcgttca
    10921 tcgcctccaa ggctcgatca taggtcgagc gcgtgaacag gccacgctcc acttcgaata
    10981 gagtctggtt tgtcaggcca gcgtccgaaa ccgcggtggt tttaagcatc ggaaaattga
    11041 ggacattttc gccaaaaatc gaccgcagat aacctaccat ttggttctgt ggtccgtcgc
    11101 tcggttcgaa acgggttatc agatagcgca tccaattaaa cttgaacttg gcgccagcat
    11161 tctcgatttc acgcaaaagg ttcgatgtca ttgccagaaa ctggttcatc gacatcacat
    11221 ccagcatctg cggatggacc gtgacaagaa tggacgtcgc cgcagtcaat gcggatagcg
    11281 tgagataccc aagctgggga gggcagtcga tgaccacgac gtcatagtta tccgcgatat
    11341 cttcaattac ttggctgatg cgaccataaa agagcgtgtc gccctctttg cggttcatca
    11401 gcgcgcgtgg cgtatcgtgt tcaaactcca tcagctcaag gttaccagga atcaggtgga
    11461 ggtcgggaat gtaagtccct cggacgactc gttcgattgc cacctgctca tcatcatacc
    11521 ttatagcgcc gtagagcgtt tcgttcgggc caacgtccgt ctccggttgg ctcccaaaga
    11581 gtgcagaaag gctcgcttga ggatcgagat caatggccaa gactcgatat ccgcgcatag
    11641 cgaggtactg cgccagatgc gcggcggtgg tggtcttacc cgacccacct ttgaaattca
    11701 tcacagagat aacctgaagc tgctcgccgc ctcgacgatg tggcaggtag cgccggttcc
    11761 cgcggccgac ctgatccata tacttccgaa tcacatggat atcttcaatt gagaacattc
    11821 gcctgccacc tgggctcatg ctaacattca actctggcat ctcagacgcg gtctgccgta
    11881 aatatgactc gccaacgccg agcagcttgg acgcctccga tggcccgaat gttcgaatac
    11941 ccttctcgga atgcggcggg aaaaccttaa gatgatgtgc ttgaagttgg ctcgagaggg
    12001 catcggcatg acgctccatc aaggccgtca accctacaac tacaggcgct gcttttagga
    12061 cagacttcgc catctcaaac ccattccttg ccagtggcga tatttttcgc gaaactggaa
    12121 aagttccgcc gctggcaatt agcgccgatt ctgctgtttg ggcaagagct tttaggttaa
    12181 cagaaggtta acgccctcag gtcgaaaaac tccacccaac tgttatttgt atttatttcc
    12241 aatgccttag agagattgcc atttgaatat gttcatgtat tgttttagtg ataatcctac
    12301 aatcgtaacc caaaaagagg tcgccctctg cgcgccgtcg tccaatatag gcgaagtcac
    12361 ccttgcgact caggcggatt ctaccttgta ggatcgagtc aggcaactat ggatgaacga
    12421 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    12481 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    12541 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    12601 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    12661 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    12721 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    12781 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    12841 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    12901 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    12961 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    13021 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    13081 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    13141 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    13201 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    13261 gccttttgct ggccttttgc tca
//
