LOCUS       sequence-232109-       13959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express the SOMA FRET sensor with a nuclear localization 
            signal in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, 
            Zhang S, Krysan PJ
  TITLE     A Forster resonance energy transfer sensor for live-cell imaging of 
            mitogen-activated protein kinase activity in Arabidopsis.
  JOURNAL   Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164.
  PUBMED    30444549
REFERENCE   2  (bases 1 to 13959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Nov 16. doi: 10.1111/tpj.14164."
COMMENT     Sequence Label: pSOMA-NLS
FEATURES             Location/Qualifiers
     source          1..13959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(673..675,676..678,679..1386)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             1822..2169
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             1918..2265
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGXGSAGGSAGGSASAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2014..2361
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSASAGGSAGGSAGGSAGGSAGGXGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2110..2457
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGXGSAGGSAGGSASAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2206..2553
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGXGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(2809..2811,2812..2814,2815..3327)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             3529..3549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3574..3826
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3862..3879)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3862..3878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3871..3893)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4081..4105
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5406..6035
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6464..7537
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7603..7797
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8033..8055)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8141..8281
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8196..8215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8296..8313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8467..9055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8547..8566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10341..11135)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11560..11584
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11662..11836
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11877..12902)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12969..13645)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(13012..13032)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    13836..13857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13872..13877,13878..13895,13896..13902)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13910..13926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13915..13937
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13934..13950
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13934..13950
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cggtacccgg ggatccttgt cgacgagtca gtaataaacg gcgtcaaagt
       61 ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca
      121 acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg
      181 tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct
      241 cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt
      301 cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag
      361 gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa
      421 tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg
      481 tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc atccgatttg
      541 ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc
      601 tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta
      661 acagaattcg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      721 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      781 gatgccacct acggcaagct gaccctgaag cttctatgca ccaccggcaa gctgcccgtg
      841 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      901 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      961 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1021 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1081 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1141 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1201 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1261 cccgacaacc actacctgag ctaccagtcc gccctgttca aagaccccaa cgagaagcgc
     1321 gatcacatgg tcctgctgga gttcctgacc gccgccggga tcactgaggg catgaacgag
     1381 ctgtacctcg agaagttttc tcaagaacag atcggcgaaa acattgtgtg cagggtcatt
     1441 tgtaccacgg gtcaaattcc catccgagat ttgtcagctg atatttcaca agtgcttaag
     1501 gaaaaacgat ccataaagaa agtttggaca tttggtagaa acccagcctg tgactatcat
     1561 ttaggaaaca tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt
     1621 aaccttttat tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag
     1681 aagaacagca atcagttact gtcccaaggt gatgaaataa ccgttggtgt aggcgtggaa
     1741 tcagatattt tatctctggt cattttcata aacgacaaat ttaagcagtg cctggagcag
     1801 aacaaagttg atcgcggtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     1861 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt
     1921 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc
     1981 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt
     2041 gctggtggta gtgctggtgg tagtgctggt ggttccggca gtgctggtgg tagtgctggt
     2101 ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     2161 gctggtggtt scggcagtgc tggtggtagt gctggtggta gtgcyagtgc tggtggtagt
     2221 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggttscgg cagtgctggt
     2281 ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     2341 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt
     2401 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc
     2461 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt
     2521 gctggtggta gtgctggtgg tagtgctggt ggttccggac ctggttctaa gaaaatgttt
     2581 tggcggtctg cctcttggtc tgcttcacgg actgcatcac aagttcctga gggtgatgag
     2641 caaagcctga acattccgtg tgctattagt tctgggccga gtcgaagatg tccagctgct
     2701 cctttgacac ctcgtgatca tcataacagc aaggctagag cttgtttgcc accattgcag
     2761 cctcttgcca tttctaggag gagcttagac gagtggcctg gcggccgcat ggtgagcaag
     2821 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2881 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2941 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3001 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     3061 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3121 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3181 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3241 aactacatca gcgggaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     3301 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     3361 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3421 cagtccgcct taagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3481 ttgaccgccg ccgggatcac tctcggcatg gacgagctgt ctagacttcc taagaagaag
     3541 agaaaggttg gaggatagtc gacgaatttc cccgatcgtt caaacatttg gcaataaagt
     3601 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     3661 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     3721 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3781 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggag atctgtcgac
     3841 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3901 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
     4021 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     4081 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     4141 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     4201 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
     4261 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
     4321 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
     4381 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
     4441 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
     4501 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
     4561 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
     4621 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
     4681 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
     4741 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
     4801 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
     4861 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
     4921 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
     4981 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
     5041 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
     5101 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
     5161 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg
     5221 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
     5281 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     5341 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
     5401 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     5461 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     5521 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     5581 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     5641 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     5701 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     5761 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
     5821 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
     5881 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
     5941 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
     6001 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
     6061 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
     6121 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
     6181 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
     6241 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
     6301 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
     6361 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc
     6421 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc
     6481 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
     6541 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
     6601 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
     6661 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
     6721 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
     6781 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
     6841 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
     6901 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
     6961 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
     7021 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
     7081 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
     7141 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
     7201 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
     7261 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
     7321 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
     7381 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
     7441 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
     7501 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
     7561 aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat
     7621 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
     7681 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
     7741 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
     7801 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
     7861 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
     7921 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
     7981 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     8041 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     8101 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     8161 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     8221 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     8281 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8881 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9121 tttggtcatg cattctaggg aaggtgcgaa caagtccctg atatgagatc atgtttgtca
     9181 tctggagcca tagaacaggg ttcatcatga gtcatcaact taccttcgcc gacagtgaat
     9241 tcagcagtaa gcgccgtcag accagaaaag agattttctt gtcccgcatg gagcagattc
     9301 tgccatggca aaacatggtg gaagtcatcg agccgtttta ccccaaggct ggtaatggcc
     9361 ggcgacctta tccgctggaa accatgctac gcattcactg catgcagcat tggtacaacc
     9421 tgagcgatgg cgcgatggaa gatgctctgt acgaaatcgc ctccatgcgt ctgtttgccc
     9481 ggttatccct ggatagcgcc ttgccggacc gcaccaccat catgaatttc cgccacctgc
     9541 tggagcagca tcaactggcc cgccaattgt tcaagaccat caatcgctgg ctggccgaag
     9601 caggcgtcat gatgactcaa ggcaccttgg tcgatgccac catcattgag gcacccagct
     9661 cgaccaagaa caaagagcag caacgcgatc cggagatgca tcagaccaag aaaggcaatc
     9721 agtggcactt tggcatgaag gcccacattg gtgtcgatgc caagagtggc ctgacccaca
     9781 gcctggtcac caccgcggcc aacgagcatg acctcaatca gctgggtaat ctgctgcatg
     9841 gagaggagca atttgtctca gccgatgccg gctaccaagg ggcgccacag cgcgaggagc
     9901 tggccgaggt ggatgtggac tggctgatcg ccgagcgccc cggcaaggta agaaccttga
     9961 aacagcatcc acgcaagaac aaaacggcca tcaacatcga atacatgaaa gccagcatcc
    10021 gggccagggt ggagcaccca tttcgcatca tcaagcgaca gttcggcttc gtgaaagcca
    10081 gatacaaggg gttgctgaaa aacgataacc aactggcgat gttattcacg ctggccaacc
    10141 tgtttcgggc ggaccaaatg atacgtcagt gggagagatc tcactaaaaa ctggggataa
    10201 cgccttaaat ggcgaagaaa cggtctaaat aggctgattc aaggcattta cgggagaaaa
    10261 aatcggctca aacatgaaga aatgaaatga ctgagtcagc cgagaagaat ttccccgctt
    10321 attcgcacct tccctaggta ctaaaacaat tcatccagta aaatataata ttttattttc
    10381 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
    10441 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
    10501 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
    10561 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
    10621 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
    10681 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
    10741 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
    10801 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
    10861 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
    10921 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
    10981 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
    11041 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
    11101 tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac
    11161 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
    11221 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
    11281 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
    11341 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
    11401 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
    11461 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
    11521 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
    11581 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat
    11641 taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
    11701 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
    11761 ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca
    11821 cattattatg gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat
    11881 ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
    11941 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
    12001 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
    12061 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt
    12121 cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
    12181 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
    12241 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
    12301 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
    12361 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
    12421 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
    12481 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
    12541 gcatccatag cctccgcgac cggttgtaga acagcgggca gttcggtttc aggcaggtct
    12601 tgcaacgtga caccctgtga acggcgggag atgcaatagg tcaggctctc gctaaactcc
    12661 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
    12721 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
    12781 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
    12841 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
    12901 atatctcatt gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac
    12961 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
    13021 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
    13081 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    13141 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    13201 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    13261 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    13321 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    13381 tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    13441 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    13501 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    13561 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    13621 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    13681 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata
    13741 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    13801 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    13861 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    13921 taacaatttc acacaggaaa cagctatgac atgattacg
//
