LOCUS       sequence-232118-       10689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetOFF (promoter-less) cassette with Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1252_TetOFF(Hyg)
FEATURES             Location/Qualifiers
     source          1..10689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             633..653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      893..1145
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1795..2820)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    3485..3503
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    3691..3709
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(3870..3886)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3870..3886)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3914..3931)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3914..3930)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3923..3945)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4133..4157
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5458..6087
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6516..7589
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7655..7849
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8085..8107)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8193..8333
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8248..8267
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8348..8365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8519..9107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8599..8618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9194..9988)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10413..10437
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10515..10689
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt catggcccgc ctggataagt caaaggtcat caactctgcc ttggagctgc
       61 tgaacgaggt cggcatcgag ggtctcacaa cgaggaagct ggcccagaag ctgggtgtcg
      121 agcagccaac cctctactgg cacgtcaaga acaagcgcgc cctcctggac gccctcgcca
      181 tcgagatgct ggaccgccac cacacccact tctgccccct ggagggcgag agctggcagg
      241 acttcctgcg caacaacgcc aagagcttca ggtgcgccct cctgagccac agggacggcg
      301 ccaaggtcca cctcggcacc cgccccaccg agaagcagta cgagaccctg gagaaccagc
      361 tcgccttcct gtgccagcag ggcttcagcc tggagaacgc cctctacgcc ctgagcgccg
      421 tcggtcactt caccctgggt tgcgtcctgg aggaccagga gcaccaggtc gccaaggaag
      481 agcgcgagac cccgaccacc gacagcatgc ccccgctcct gcgccaggcc atcgagctgt
      541 tcgaccacca gggtgccgag cccgccttcc tgttcggcct tgagcttatc atctgcggcc
      601 tggagaagca gctgaagtgc gagagcggct cgcccaagaa gaagcgcaag gtcgagggca
      661 gcgtcagcat gcagccaagc ctcaggagcg agtacgagta cccggtcttc agccacgtcc
      721 aggccggcat gttcagcccc gagctgcgca ccttcaccaa gggtgacgcc gagcgctggg
      781 tcagcggctc gccggccgac gccctggatg atttcgacct ggatatgctc cctgccgacg
      841 ccctggacga cttcgacctg gatatgctgt gaggcgcggc cggccgcccg gcgatcgttc
      901 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
      961 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     1021 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     1081 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     1141 agatccgatg ataagctgtc aaacatgaga attgcggccg cgtcgacaag aaggattacc
     1201 tctaaacaag tgtacctgtg cattctgggt aaacgactca taggagagtt gtaaaaaagt
     1261 ttcggccggc gtattgggtg ttacggagca ttcactaggc aaccatgcat ggttactatt
     1321 gtatacccat cttagtagga atgattttcg aggtttatac ctacgatgaa tgtgtgtcct
     1381 gtaggcttga gagttcaagg aagaaacagt gcaattatct ttgcgaaccc aggggctggt
     1441 gacggaattt tcatagtcaa gctatcagag taaagaagag gagcatgtca aagtacaatt
     1501 agagacaaat atatagtcgc gtggagccaa gagcggattc ctcagtctcg taggtctctt
     1561 gacgaccgtt gatctgcttg atctcgtctc ccgaaaatga aaatagactc tgctaagcta
     1621 ttcttctgct tcgccggagc ctgaagggcg tactagggtt gcgaggtcca atgcattaat
     1681 gcattgcaga tgagctgtat ctggaagagg taaacccgaa acgcatttta ttcttgttga
     1741 catggagcta ttaaatcact agaattactc gactggttcc ggtcggcatc tactctattt
     1801 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     1861 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     1921 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     1981 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
     2041 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     2101 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     2161 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     2221 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     2281 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     2341 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     2401 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     2461 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg
     2521 caacgtgaca ccctgtgaac ggcgggagat gcaataggtc aggctctcgc taaactcccc
     2581 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     2641 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     2701 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     2761 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     2821 ttggatgctt gggtagaata ggtaagtcag attgaatctg aaataaaggg aggaagggcg
     2881 aacttaagaa ggtatgaccg ggtcgtccac ttaccttgct tgacaaacgc accaagttat
     2941 cgtgcaccaa gcagcagatg ataataatgt cctcgttcct gtctgctaat aagagtcaca
     3001 cttcgagcgc cgccgctact gctacaagtg gggctgatct gaccagttgc ctaaatgaac
     3061 catcttgtca aacgacacaa attttgtgct caccgcctgg acgactaaac caaaataggc
     3121 attcattgtt gacctccact agctccagcc aagcccaaaa agtgctcctt caatatcctc
     3181 gactctagat aaatcattcc actcaacatt caggctcctc tgcgcacgta aagtgccaaa
     3241 ggcaataccc tgctcggtgg aatgccgccg ggcttgtcga ttttacgcac ataggcgcat
     3301 tcttgacttg aagcggagga gttcttcgtt gcgggttaca gtgttttaat aaaagaatgg
     3361 tcaaatcaaa ctgctagata tacctgtcag acactctagt tgttgacccc tatactctta
     3421 atacatcaga cagtacatgc atgttgcatg atgatgtcgg tacaactccc actagacata
     3481 tcctactcta tcattgatag agtgaccctt ccatagacat atcctactct atcattgata
     3541 gattgaccct tccactaggt tcacttttct ctatcattga tagggagtgg taaacaattg
     3601 atggacaaaa tctatcaatg atagagagtg catgttacct agtattgttc ttctctatca
     3661 ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt ctagtattag
     3721 caaggccttt cctcagatcc cgatcaatcc gacagccaaa cctcatccac tctcactttc
     3781 acactctaac ttatacgatc acttctcgcc cgttcttttg aacatcaaat caactacctt
     3841 acttctatca attaatatgg gtacccgggg atccactagt tctagagtcg acctgcaggc
     3901 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3961 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4021 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
     4081 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     4141 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     4201 gggcgtgaaa aggtttatcc gttggtccat ttgtatgtcc atgccaacca cagggttccc
     4261 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     4321 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     4381 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     4441 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     4501 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     4561 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     4621 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     4681 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     4741 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     4801 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     4861 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     4921 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     4981 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     5041 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     5101 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     5161 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     5221 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
     5281 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     5341 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     5401 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     5461 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     5521 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     5581 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     5641 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     5701 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     5761 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     5821 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     5881 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     5941 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     6001 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     6061 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     6121 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     6181 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     6241 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     6301 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     6361 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     6421 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc
     6481 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
     6541 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     6601 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     6661 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     6721 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     6781 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     6841 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     6901 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     6961 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     7021 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     7081 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     7141 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     7201 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     7261 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     7321 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     7381 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     7441 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     7501 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     7561 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     7621 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     7681 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     7741 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     7801 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     7861 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     7921 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     7981 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     8041 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     8221 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8281 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     8341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8941 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9181 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     9241 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     9301 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     9361 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     9421 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     9481 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     9541 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     9601 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     9661 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     9721 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     9781 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     9841 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     9901 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     9961 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tatagtattt
    10021 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    10081 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    10141 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
    10201 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
    10261 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
    10321 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
    10381 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
    10441 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
    10501 gaattatgtc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
    10561 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
    10621 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
    10681 atggagaaa
//
